Biosynthesis and effects of copper nanoparticles on plants (original) (raw)
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In vitro effects of copper nanoparticles on plant pathogens, beneficial microbes and crop plants
Spanish Journal of Agricultural Research
Copper-based chemicals are effectively used as antimicrobials in agriculture. However, with respect to its nanoparticulate form there has been limited number of studies. In this investigation, in vitro tests on effect of copper nanoparticles (CuNPs) against plant pathogenic fungi, oomycete, bacteria, beneficial microbes Trichoderma harzianum and Rhizobium spp., and wheat seeds were conducted. Integration of CuNPs with non-nano copper like copper oxychloride (CoC) at 50 mg/L concentration each recorded 76% growth inhibition of the oomycete Phytophthora cinnamomi in vitro compared to the control. CuNPs also showed synergistic inhibitory effect with CoC on mycelial growth and sporulation of A. alternata. Pseudomonas syringae was inhibited at 200 mg/L of CuNPs. CuNPs were not significantly biocidal against Rhizobium spp. and Trichoderma harzianum compared to CoC. Evaluation of the effect of CuNP on wheat revealed that rate of germination of wheat seeds was higher in presence of CuNPs an...
Bactericides Based on Copper Nanoparticles Restrain Growth of Important Plant Pathogens
Pathogens
Copper nanoparticles (CuNPs) can offer an alternative to conventional copper bactericides and possibly slow down the development of bacterial resistance. This will consequently lower the accumulation rate of copper to soil and water and lower the environmental and health burden imposed by copper application. Physical and chemical methods have been reported to synthesize CuNPs but their use as bactericides in plants has been understudied. In this study, two different CuNPs products have been developed, CuNP1 and CuNP2 in two respective concentrations (1500 ppm or 300 ppm). Both products were characterized using Dynamic Light Scattering, Transmission Electron Microscopy, Attenuated Total Reflection measurements, X-ray Photoelectron Spectroscopy, X-ray Diffraction and Scattering, and Laser Doppler Electrophoresis. They were evaluated for their antibacterial efficacy in vitro against the gram-negative species Agrobacterium tumefaciens, Dickeya dadantii, Erwinia amylovora, Pectobacterium...
A novel method of management of maize pathogens in vitro and in vivo using newly synthesized copper nanoparticles (CuNPs) has been documented in this study. CuNPs have been synthesized using CuSO4 as a precursor, NaBH4 and ascorbic acid as a reducing agent, and polyethylene glycol 8000 (PEG-8000) as a stabilizing agent. Characterization of CuNPs using a Transmission Electron Microscope (TEM) confirmed the nanoparticles' size range of 35–70 nm. Fourier transform infrared spectroscopy (FTIR) revealed the association of alcohol groups and allyl halides group with CuNPs. The synthesized CuNPs exhibited significant inhibition at 20 ppm of three pathogenic fungi namely Macrophomina phaseolina, Bipolaris maydis, and Fusarium verticillioides, and at 50 ppm against Rhizoctonia solani. Bactericidal property of CuNPs was evidenced against Erwinia carotovora and Ralstonia solanacearum at 30 ppm. Evaluation of CuNPs in vivo against two diseases viz., maydis leaf blight (MLB) and banded leaf ...
In vitro antifungal efficacy of copper nanoparticles against selected crop pathogenic fungi
Materials Letters, 2014
Copper nanoparticles play an important role in the field of optics and electronics, and also as a novel antimicrobial. In the present study, we report antifungal activity of copper nanoparticles against selected crop pathogenic fungi. Copper nanoparticles were synthesized by chemical reduction of Cu 2 þ in the presence of Cetyl Trimethyl Ammonium Bromide and isopropyl alcohol. Characterizations of copper nanoparticles were carried out by UV-visible spectroscopy, nanoparticles tracking analysis (NTA), Fourier transform infrared spectroscopy (FTIR) and transmission electron microscopy (TEM) which revealed that synthesized nanoparticles were coated by Cetyl Trimethylammonium Bromide (CTAB) having particle size of 3-10 nm. Copper nanoparticles demonstrated significant antifungal activity against plant pathogenic fungi: Phoma destructiva (DBT-66), Curvularia lunata (MTCC no. 2030), Alternaria alternata (MTCC No. 6572) and Fusarium oxysporum (MTCC No. 1755). Since for the synthesis of copper nanoparticles the present chemical method by using C-TAB-IPA is found to be simple, economic and fast, the synthesized copper nanoparticles can be used as a novel antifungal agent in agriculture to control the plant pathogenic fungi as well as potent disinfectant in poultry and animal husbandry.
Phytosynthesis of Copper Nanoparticles Using Extracts of Spices and Their Antibacterial Properties
Processes, 2021
To prevent microbial growth, chemical solvents are typically utilized. However, chemical solvents are hazardous to human health with low antimicrobial effects. Metallic-element (such as copper, silver, and gold) nanoparticles have many applications in biotechnology and biomedicine. Copper nanoparticles (CuNPs) are efficient owing to their antimicrobial, anti-inflammatory, and anti-proliferative properties. The objective of this study was to perform biogenic synthesis of copper nanoparticles using three different spices (star anise, seed of Illicium verum; nutmeg, seed of Myristica fragrans; and mace, membrane covering the seed of Myristica fragrans) and determine their antibacterial properties. CuNPs of spices were prepared by dissolving copper sulfate in the respective plant extract. They were then characterized by UV-Vis spectroscopy, FTIR, GC-MS, EDAX, and SEM analysis. Results of UV-Vis spectroscopy showed the maximum absorbance peak at 350 nm. SEM analysis showed that the sizes...
Frontiers in Chemistry, 2023
Copper oxide nanoparticles (CuO-NPs) have piqued the interest of agricultural researchers due to their potential application as fungicides, insecticides, and fertilizers. The Serratia sp. ZTB29 strain, which has the NCBI accession number MK773873, was a novel isolate used in this investigation that produced CuO-NPs. This strain can survive concentrations of copper as high as 22.5 mM and can also remove copper by synthesizing pure CuO-NPs. UV-VIS spectroscopy, DLS, Zeta potential, FTIR, TEM, and XRD techniques were used to investigate the pure form of CuO-NPs. The synthesized CuO-NPs were crystalline in nature (average size of 22 nm) with a monoclinic phase according to the XRD pattern. CuO-NPs were found to be polydisperse, spherical, and agglomeration-free. According to TEM and DLS inspection, they ranged in size from 20 to 40 nm, with a typical particle size of 28 nm. CuO-NPs were extremely stable, as demonstrated by their zeta potential of −15.4 mV. The ester (C=O), carboxyl (C=O), amine (NH), thiol (S-H), hydroxyl (OH), alkyne (C-H), and aromatic amine (C-N) groups from bacterial secretion were primarily responsible for reduction and stabilization of CuO-NPs revealed in an FTIR analysis. CuO-NPs at concentrations of 50 μg mL −1 and 200 μg mL −1 displayed antibacterial and antifungal activity against the plant pathogenic bacteria Xanthomonas sp. and pathogenic fungus Alternaria sp., respectively. The results of this investigation support the claims that CuO-NPs can be used as an efficient antimicrobial agent and nano-fertilizer, since, compared to the control and higher concentrations of CuO-NPs (100 mg L −1) considerably improved the growth characteristics of maize plants.
Since its advent, nanotechnology has become an indispensable area of research and innovation, introducing revolutionary changes in current research areas like engineering, medical sciences, drug discovery and formulations, optoelectronics and biosensors. Metallic nanoparticle synthesis has become a requisite of modern experimentation however, much of the research involves either silver or gold nanoparticles. Considering the fact, it is imperative to explore the potentials of other metallic nanoparticles as well. Current review focuses on exploration of potentials of Copper nanoparticles in various domains of research. The review highlights common synthesis methods of Copper nanoparticle synthesis and the superiority of green route over other approaches. Characterization techniques and multifarious biological potentials of Copper nanoparticles have also been reviewed. Researchers have recognized green synthesis route as the best alternative to traditional methods utilized for synthesizing copper nanoparticles. Green synthesized Copper nanoparticles have also been found to have superior antioxidant, antimicrobial, antifungal, cytotoxic and photocatalytic potentials. As of now, the exact mechanism behind synthesis and biological activities of Copper nanoparticles is not known. Identification of the exact mechanism can revolutionaries the discipline of nanotechnology.
Copper and copper nanoparticles: role in management of insect-pests and pathogenic microbes
Nanotechnology Reviews
Crop losses mainly occur due to biotic factors, which include soil-borne phytopathogens, insect pests, parasites, and predators. The major loss of food in the food industry is due to its spoilage by various microorganisms. With advancement in nanotechnology, the use of nanoparticles in food and agriculture crop yield can be improved. In this context, copper nanoparticles (CuNPs) have attracted a great deal of attention from all over the world due to their broad-spectrum antimicrobial activity. Copper is one of the key micronutrients, which plays an important role in growth and development of plants. CuNP-based fertilizer and herbicide can be used in agriculture. The small size of CuNPs facilitates their easy absorption by the plants. CuNPs can be promisingly used in the food packaging to avoid the growth of food spoilage microorganisms. The use of CuNP-based agar packaging materials has substantial potential to increase the shelf-life of food. The present review focuses on the appli...
The plants Asparagus adscendens, Bacopa monnieri, Ocimum bacilicum, and Withania somnifera were used and compared for their extracellular synthesis of metallic copper nanoparticles (CuNPs). Stable Cu nanoparticles were formed by treating aqueous solution of CuSO4•5H2O with the plant leaf extracts as reducing agent. By treatment of 1mM aqueous solutions of CuSO4•5H2O with leaf extract stable CuNPs were formed; the change in color of solution confirm the formation of stable nanoparticles. UV-Visible study revealed qualitative formation of CuNPs and characteristic absorption peak in Asparagus adscendens, Bacopa monnieri and Ocimum bacilicum leaf extract at the range of 500-700nm but in Withania somnifera leaf extract absorption peak of CuNPs is shifted at the range of 500-800nm. These biosynthesized CuNPs were characterized with the help of Fourier transform infrared spectroscopy (FTIR), and Transmission Electron Microscopy (TEM). The involvement of primary and secondary metabolites or possible reducing agent confirmed by FTIR analysis. TEM confirms the formation and the crystalline nature of Copper nanomaterial. This method can be used as effective and environmental friendly technique for the synthesis of Copper nanoparticles using leaf extract of different plants. The antibacterial potentials of the CuNPs were studied and these are shown good antimicrobial activity against Gram positive and Gram negative bacteria.