Mitochondria-rich cells adjustments and ionic balance in the Neotropical fish Prochilodus lineatus exposed to titanium dioxide nanoparticles (original) (raw)

2018, Aquatic toxicology (Amsterdam, Netherlands)

Manufactured titanium dioxide nanoparticles (TiO-NP) have been intensely applied in numerous industrial products and may be a risk for aquatic systems as they are not completely removed from domestic and industrial wastes after water treatment. This study evaluated the osmo- and ionic balance, Na/K-ATPase, H-ATPase and carbonic anhydrase activities and the mitochondria-rich cells (MRC) in the gills and kidney of the Neotropical fish Prochilodus lineatus after 2 (acute) and 14 (subchronic) days of exposure to nominal 0, 1, 5, 10 and 50 mg L TiO-NP. The nominal concentrations corresponded to 0.0, 0.6, 1.6, 2.7 and 18.1 mg L suspended TiO-NP, respectively, in the water column one hour after NP introduction and were maintained for at least 24 h. Acute exposure to TiO-NP decreased plasma osmolality and Ca levels. Na/K-ATPase, H-ATPase and carbonic anhydrase activities were inhibited in the gills, but not in the kidney. Total MRC density did not change in gills and kidneys. At gill surfac...