Effects of Extracts from Medicinal Plants on Biofilm Formation byEscherichia ColiUrinary Tract Isolates (original) (raw)

2011, Biotechnology & Biotechnological Equipment

Materials and Methods Strains, media and cultivation In this study were included three urinary clinically isolated strains with pre-estimated good biofilm growth capacity (11) from the laboratory collection of the Departnemt of Biochemistry and Microbiology, Plovdiv University. PU-1 was isolated from a woman with pyelonephritis, PU-13-from a man with cystitis, and PU-19-from a man with ABU. Estimation of the antibacterial activity of the plant extracts was tested also on reference strains: E. coli ATCC 25922, Pseudomonas aeruginosa ATCC 27853, Staphylococcus aureus ATCC 25923 and Streptococcus saprophyticus nctc 7292. The strains were stored frozen in Trypticase soy broth (TSB) (Difco) supplemented with 30% glycerol. Before use, samples were inoculated into TSB and incubated overnight at

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