GENETIC DIVERSITY ANALYSIS AMONG GARLIC (Allium sativum L.) GENOTYPES THROUGH MOLECULAR MARKERS (original) (raw)
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Assessment of Genetic Diversity in Garlic (Allium Sativum L.) Genotypes Based on Issr Markers
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Genetic diversity among one hundred and thirty-one accessions of garlic was analyzed using Inter-simple sequence repeat (ISSR) markers. A total of twelve polymorphic bands were detected with four primers. Genetic similarity result showed genetic variation among the garlic accessions ranged from (0.00) to (1.00) with a mean of 0.501. All 131 accessions were clustered into two major groups. The smallest genetic similarity value was observed between PG-20 and all genotypes except K1 (0.500) and W6-12840 (0.125) genotypes, which appear as the most dissimilar accessions and distantly related. The maximum genetic similarity value of 1.00 was obtained between PG-32 with F4, F5, BG-108, F2, CGF-2 genotypes. The genotypes PG-20, K1, WG-35698, Single Kale, GG-4, RG-77, CFG-3, G-335 and AC-200 are identified as more diverse among the genotypes under study could be used for the genetic improvement of garlic cultivars.
Physiology and Molecular Biology of Plants, 2018
Genetic diversity was assessed among 53 Indian garlic accessions using SSR markers. Initially, 24 SSR primer pairs were used for screening three selected garlic accessions. Out of 24 SSR primer pairs, 10 primer pairs which consistently showed good amplification and polymorphism were selected for DNA profiling. SSR primer pairs showed PIC values ranging from 0.30 to 0.99. Based on AMOVA we found that the greater part of the genetic diversity was expected due to intra population with 84% variation and only 16% of variation was due to among populations suggesting presence of genetic structure. The results of cluster analysis and principal component analysis largely correspond to each other. Population structure analysis revealed genetic differentiation of accessions. The results of present study revealed existence of significant variability in Indian garlic germplasm.
Chilean journal of agricultural research, 2008
Garlic (Allium sativum L.) is a species of vegetative propagation, showing high morphological diversity. Besides, its clones have specific adaptations to different agroclimatic regions. The objective of this study was to determine the genetic diversity of 65 garlic clones collected in Chile and introduced from different countries, by using RAPD (Random Amplified Polymorphic DNA). Fourty random primers of 10 mers generated a total of 398 bands with an 87% of polymorphism. Each primer amplified between two and 20 bands. The size of the fragments obtained fluctuated between 3200 and 369 bp. The results showed that the clones analyzed had a genetic similarity rate of 94%. In addition, 70% of them were clustered in one major group. However, in spite of that situation several clones have different agronomic characteristics.
Classification of genetic variation in garlic (Allium sativum L.) using SSR markers
Genetic isolation of Allium sativum, 2019
Garlic bulb is an important seasoning ingredient in many of the world's cuisines. However, clonal lineages within this species show a remarkably high degree of phenotypic diversity in bulb size and color. Present study classified the genetic variations of garlic. Seven selected simple sequence repeats (SSRs) revealed a total of 37 alleles across 120 garlic accessions, with an average of seven alleles per locus. The values for observed heterozygosity ranged from 0 to 0.99 (mean = 0.71). The average genetic diversity and polymorphic information content values were 0.586 and 0.518, respectively. Based on the 37 alleles obtained from the seven SSRs, a phylogram was constructed to understand the relationships among the 120 accessions. The garlic accessions were clustered into four main groups (G1-G4) in the phylogram. Group 1 consisted of accessions of 'Aomori', Group 2 consisted of 64 accessions, Group 3 consisted of 25 accessions, and Group 4 consisted of 20 accessions. Our results indicate that genetic diversity is correlated with geographical region. There may have been local selection pressure and differences in adaptability of the garlic to different geographical conditions. All of the tested loci deviated significantly (P < 0.01) from Hardy-Weinberg equilibrium. Thus, a number of disturbances occurred in the garlic population tested, including natural selection. Our findings will help explain the genetic relationships and population structure of garlic accessions.
Assessment of genetic variability and correlation studies in garlic ( Allium sativum L . )
2018
With the aim to study genetic variability, heritability, genetic advance and correlation in eightyindigenousgarlic (Allium sativum L.) genotypes collected from different places of Uttar Pradesh. Experimental trial was conducted at Narendra Dev University of Agriculture & Technology, Kumaganj, Faizabad (U.P.) during rabi2015-2016. The experiment was conducted in augmented block design. Observations were recorded for twelve characteristics. The higher magnitude of coefficient of variation observed for width of leaf (cm), weight of clove (g), number of cloves per bulb, number of leaves per plant, plant height, length of leaf (cm) and diameter of bulb, at phenotypic levels. High heritability coupled with high genetic advance in percent of mean was recorded for number of cloves per bulb, length of leaf, Total soluble solid (T.S.S.), width of clove and bulb yield per plant. The length of leaf had highly significant and positive correlation with plant height, number of leaves per plant and...
ASSESMENT OF GENETIC DIVERSITY IN GARLIC CLONES USING SSR AND ISSR MARKERS
Sixteen Simple Sequence Repeats (SSR) and three Inter Simple Sequence Repeats (ISSR) primers were used to es-timate the genetic diversity and its distri-bution in twenty garlic clones. A high level of polymorphism amongst studied clones was found with both SSR and ISSR markers. The total number of bands that were detected by all used primers was 75 including 6 monomorphic, 5 unique and 64 polymorphic. The percentage of poly-morphism identified by SSR primers were varied between 33.3 and 100. However, all of the studied ISSR primers were pol-ymorphic conferring a 100% of polymor-phism. Results showed that each of the Asa14, Asa17, Asa18 and Asa59 primers generated one monomorphic band of 77, 120, 102 and 113 bp, respectively, in all of the studded garlic clones. Two monomor-phic bands of 104 and 177 bp were gener-ated by using Asa24 primer. Asa17 and Asa59 SSR primers produced only one unique band of 154 (Egaseed 2) and 646 bp (EGA 1), respectively. Two unique bands of 225 and 250 bp were detected for Egaseed 2 (ft) by using HB 13 ISSR pri-mer. The highest similarity value (0.969) was found between AZO 2 and AZO 3, while the lowest value (0.482) was found between AZO 4 and EGA 5 clones. Dendrogram of genetic distances amongst all tested clones showed two distinct ma-jor clusters with overlapping. In general, the present results reveal the importance of using molecular markers to assess ge-netic diversity among such closely related genotypes which were difficult to distin-guish with other markers.
Genetic Variability Assessment in garlic (Allium sativum L.) Genotypes
Journal of Pharmacognosy and Phytochemistry, 2017
The present investigation was carried out to estimate genetic variability among eighty genotypes/hybrid of Garlic for twelve characters comprised of bulb yield and its contributing characters. These genotypes were planted in augmented block design during Rabi-2015-16 at Main Experimental Station of
ANNALS OF PLANT AND SOIL RESEARCH
To assess the dispersion of genetic variation among Indian garlic accessions, twenty-five genotypically diverse accessions were studied through analysis of genetic variability, mean performance, heritability and genetic gain for 17 yield contributing agro-morphological traits.The study involving 25 Indian garlic accessions was undertaken at Vegetable Farm, CSK HPKV, Palampur during 2017-2018. Based on mean performance, Kanaid Local Selection and Chambi Local Selection were the top-ranking genotypes for bulb yield per plant which significantly out yielded all the genotypes with a significant increase of 28.7 % and 12.7 %, respectively over standard check, (GHC-1). In general, the magnitude of PCV was higher than their respective GCV for all the traits studied which reflected the considerable influence of environment on the manifestation of these traits.High estimates of phenotypic and genotypic coefficients of variation were obtained for bulbils per plant, clove weight and clove equa...
Studies on genetic divergence in garlic (Allium sativum L.) germplasm
2018
The present investigation entitled “Studies on genetic divergence in garlic germplasm. in garlic (Allium sativum L.)”. The experiment was executed at Main Experiment, Station of Department of Vegetable Science, NDUAT Kumarganj Faizabad UP during Rabi season 2015-2016, with following objectives (1) to work out direct and indirect effects of different traits on yield and (2) to work out genetic divergence in garlic germplasm. The experimental material for the present study consisted of eighty genotypes, with plot size of 2.0 × 0.60 m with distance of 30 cm row to row and 10 cm plant to plant. Observation were recorded on plant height (cm), number of leaves per plant, length of leaf (cm), width of leaf (cm), neck thickness of bulb (cm), diameter of bulb (cm), bulb yield per plant (g), number of cloves per bulb, length of clove (cm), weight of clove (g), diameter of clove (cm) and total soluble solids (%). The maximum positive direct effect on bulb yield per plant was exerted by number ...