To Study the Relationship between the Expression of MiR-21, MiR-155, Mir-182, and Mir-437 and Inflammatory Factors in the Cerebrospinal Fluid of Patients with Multiple Sclerosis (original) (raw)

Background: MicroRNAs (miRNAs) affect the differentiation and function of inflammatory cells. In addition, miRNAs can regulate the expression of pro-inflammatory genes in neuron cells. Therefore, the relationship between the expression of miRNAs and inflammatory markers in the cerebrospinal fluid of individuals with multiple sclerosis was investigated.Methods: RT-PCR determined the expression levels of MiR-21, MiR-155, Mir-182, and Mir-437 in CSF samples from patients with multiple sclerosis (MS) and the control group. The levels of the inflammatory cytokines IL -1β, IL -6, and TNF-α in CSF were measured by ELISA. The level of high-sensitivity C-reactive protein (hs-CRP) was measured by the quantitative turbidometric technique.Results: The expression of miRNAs and inflammatory factors were higher in the CSF of patients with MS than in the control group, and this difference was significant (P <0.05). The results of Receiver Operating Characteristics (ROC) analysis show that the ar...