Occurrence and associated lesions of Pasteurella multocida in porcine bronchopneumonia (original) (raw)
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Pesquisa Veterinária Brasileira, 2017
ABSTRACT: The bacterium Pasteurella multocida is a frequent cause of porcine respiratory disease complex in finishing pigs. Historically, the bacterium is recognized as an opportunistic agent, causing secondary bacterial pneumonia in pigs. Several Brazilian reports have suggested the ability of P. multocida to cause primary pulmonary infection that leads to the death of finishing pigs prior to slaughter. The aim of this study was to evaluate anatomopathological pulmonary findings associated with P. multocida infection that were obtained from animals with clinical respiratory disease and from animals at slaughter. Twenty-five lung samples from 14 herds of finishing pigs with acute clinical respiratory disease and 19 lungs collected at slaughter from a different set of 14 herds were studied. In all lung samples, bacterial isolation was performed, and only samples with pure P. multocida growth were included in the study. Gross and histopathological lesions were evaluated, as well as In...
Polyserositis Associated with Pasteurella Multocida Type A in a Crossbred Pig
International Journal of Livestock Research, 2017
Pasteurella multocida is the causative agent of wide range of diseases in animals and birds. A crossbred 5 month old female pig presented to the post mortem facility with a history of respiratory distress was investigated to determine the cause of death. Grossly there were fibrinous pleuro pneumonia, pericarditis, fibrinous peritonitis with adhesion of abdominal visera to abdominal wall. Histologically there was non suppurative bronchopneumonia. Fibrosis was noticed on surface of lung, heart and liver. Pericarditis, myocardial degeneration and endocarditis were noticed. Gram negative, cocco-bacillary organism was isolated in 5 % sheep blood agar from heart blood, heart and spleen. The blood smears and tissue impressions revealed bipolar staining coccobacillary organisms. These colonies were confirmed as Pasteurella multocida type A by type specific PCR assay. Antibiotic sensitivity of this isolate showed susceptibility to to ampicillin, chloramphenicol, ciprofloxacin, tetracycline and gentamycin and found resistant to cephalexin, oxytetracycline, furazolidone and nalidixic acid.
Pasteurella multocida type A as the primary agent of pneumonia and septicaemia in pigs
Pesquisa Veterinária Brasileira, 2015
In order to understand better the pathological aspects and spread of Pasteurella multocida type A as the primary cause of pneumonia in pigs, was made an experiment with intranasal inoculation of different concentrations of inocula [Group (G1): 10 8 Colony Forming Units (CFU)/ml; G2: 10 7 CFU/ml; G3: 10 6 CFU/ml and G4: 10 5 CFU/ml], using two pigs per group. The pigs were obtained from a high health status herd. Pigs were monitored clinically for 4 days and subsequently necropsied. All pigs had clinical signs and lesions associated with respiratory disease. Dyspnoea and hyperthermia were the main clinical signs observed. Suppurative cranioventral bronchopneumonia, in some cases associated with necrosuppurative pleuropneumonia, fibrinous pericarditis and pleuritic, were the most frequent types of lesion found. The disease evolved with septicaemia, characterized by septic infarctions in the liver and spleen, with the detection of P. multocida type A. In this study, P. multocida type A strain #11246 was the primary agent of fibrinous pleuritis and suppurative cranioventral bronchopneumonia, pericarditis and septicaemia in the pigs. All concentrations of inoculum used (10 5-10 8 CFU/ml) were able to produce clinical and pathological changes of pneumonia, pleuritis, pericarditis and septicemia in challenged animals.
Acta veterinaria Hungarica, 2015
This is the first report of Pasteurella multocida type B in Hungarian pigs. This disease was observed in backyard-raised pigs in three households within a small area. Neither the source of the infection nor the epidemiological connection between any of the premises could be determined. The most consistent lesion was dark red discolouration of the skin of the ventral neck and brisket, with accompanying oedema and haemorrhages. The morbidity was low and lethality relatively high, with three dead (50%) and two euthanised (33%) out of six affected animals. A total of three isolates of P. multocida (P55, P56 and P57) were cultured from these cases and examined in detail. These were identified as P. multocida ssp. multocida biovar 3. All were toxA negative and belonged to serotype B:2. Multilocus sequence typing was used to assign these to a new sequence type (ST61) that is closely related to other haemorrhagic septicaemia causing strains of P. multocida regardless of the host. M13 polyme...
Pathogenic variability among Pasteurella multocida type A isolates from Brazilian pig farms
BMC Veterinary Research, 2018
Background: Pasteurella multocida type A (PmA) is considered a secondary agent of pneumonia in pigs. The role of PmA as a primary pathogen was investigated by challenging pigs with eight field strains isolated from pneumonia and serositis in six Brazilian states. Eight groups of eight pigs each were intranasally inoculated with different strains of PmA (1.5 mL/nostril of 10e7 CFU/mL). The control group (n = 12) received sterile PBS. The pigs were euthanized by electrocution and necropsied by 5 dpi. Macroscopic lesions were recorded, and swabs and fragments of thoracic and abdominal organs were analyzed by bacteriological and pathological assays. The PmA strains were analyzed for four virulence genes (toxA: toxin; pfhA: adhesion; tbpA and hgbB: iron acquisition) by PCR and sequencing and submitted to multilocus sequence typing (MLST). Results: The eight PmA strains were classified as follows: five as highly pathogenic (HP) for causing necrotic bronchopneumonia and diffuse fibrinous pleuritis and pericarditis; one as low pathogenic for causing only focal bronchopneumonia; and two as nonpathogenic because they did not cause injury to any pig. PCR for the gene pfhA was positive for all five HP isolates. Sequencing demonstrated that the pfhA region of the HP strains comprised four genes: tpsB1, pfhA1, tpsB2 and pfhA2. The low and nonpathogenic strains did not contain the genes tpsB2 and pfhA2. A deletion of four bases was observed in the pfhA gene in the low pathogenic strain, and an insertion of 37 kb of phage DNA was observed in the nonpathogenic strains. MLST clustered the HP isolates in one group and the low and nonpathogenic isolates in another. Only the nonpathogenic isolates matched sequence type 10; the other isolates did not match any type available in the MLST database. Conclusions: The hypothesis that some PmA strains are primary pathogens and cause disease in pigs without any co-factor was confirmed. The pfhA region, comprising the genes tpsB1, tpsB2, pfhA1 and pfhA2, is related to the pathogenicity of PmA. The HP strains can cause necrotic bronchopneumonia, fibrinous pleuritis and pericarditis in pigs and can be identified by PCR amplification of the gene pfhA2.
International Journal of Sciences, 2013
Pasteurella multocida is considered a important pathogens of swine respiratory system, causing progressive atrophic rhinitis, septicemia and pneumonia in pigs. The aim of this study is to quantify Pasteurella multocida in lungs of slaughtered pigs with and without pneumonia by qPCR technique and evaluate the interactions between bacteria and viruses by analyzing the association and the correlation between them using qPCR, microbiological and histopathological examinations of the lungs. Of the 109 samples collected, 61 samples showed no macroscopic lesions and 48 samples with macroscopic lesions. A total of 45.8% (22/48) of lung lesion group and 4.9% (3/61) of the group without lesions were positive for P. multocida isolation, being the only significant association with the lesion and control groups (p <0.000). Based on the number of copies of the kmt1 gene of P. multocida present in the lesion group (8.25 copies) and in the control group (1.6 copies) there is a significant difference (p <0.05). The qPCR technique for detection of P. multocida was efficient for being more sensitive than the microbiological method, demonstrating that a larger number of kmt1 gene copies is in GL suggest that P. multocida is an important agent in the development of pneumonia.
The Prevalence of Pasteurella Multocida from Farm Pigs in Serbia
Macedonian Veterinary Review, 2016
The investigations covered a total of 234 lungs from necropsied pigs with different pneumonic lesions, from 6 farrow-to-finish pig farms during 2013 and 2014. The samples were inoculated on selective culture media and aerobically incubated at 37°C and in carbon dioxide condition. The isolated bacterial colonies were further characterised morphologically and biochemically. The identification was confirmed using the BBL Crystal, E/N, G/P ID Kit (Becton Dickinson). For determination of the type of
ISOLATION AND CHARACTERIZATION OF Pasteurella multocida FROM CAPRINE PNEUMONIC LUNGS
By conventional microbiological methods, investigation was carried out in Nsukka and Enugu areas of Enugu State, Nigeria to determ ne the percentage frequency of occurrence of Pasteurella multocida from caprine pneumonic lungs in Nsukka and Enugu areas of Enugu State, Nigeria. In this study that spanned 12 years, a total of 350 pneumonic lung samples were collected from West African Dwarf goats, Sokoto Red goats and Fulani goats slaughtered in Nsukka and Enugu Municipal abattoirs in Enugu State. By cultural, biochemical and physiological attributes, four (4) of the isolates were characterized as Pasteurella multocida Both gross and histopathological lesions of the pneumonic lung specimens from which this aerobic bacterium was isolated were correlated with the organism. Inspite of the low percentage requency (1 14%) of isolation of Pasteurella multocida in this study, attention is drawn to the pathogenic potential of this organism for goats and other livestock in this par o Nigeria. i f. t f t
Journal of Comparative Pathology, 2010
Pasteurella multocida A:3 is a common cause of suppurative bronchopneumonia in calves and results in significant production losses and mortality. Here we describe the lesions in three calves at each of four time points (1 day and 4, 7 and 10 days) after experimental intratracheal infection with approximately 1 Â 10 9 colony-forming units of P. multocida A:3 Moredun Research Institute (MRI isolate 671/90). Equivalent age-and time-matched sham-dosed negative control animals were also studied. Infected calves developed significantly elevated mean rectal temperatures (P < 0.001) and respiratory rates (P < 0.001) compared with negative control animals. Extensive consolidation of multiple lung lobes was present on each of the day/s post-infection (dpi). Histologically, large numbers of alveoli contained either or both polymorphonuclear neutrophils (PMNs) and oedema fluid (1 dpi). At 4 dpi a severe fibrinosuppurative bronchopneumonia had developed. At this time, PMNs and macrophages formed focal lesions containing central necrotic and mineralized debris, while the interlobular septa were severely distended by oedema. Early abscess formation was present in the lung parenchyma at 7 dpi and many of the interlobular septa were thrombosed. At 10 dpi abscesses within the lung parenchyma were mature and comprised of central necrosis with surrounding layers of PMN, macrophages and fibrous tissue. This study describes, for the first time, the commencement, nature and progression of lesions in bovine pneumonic pasteurellosis caused by P. multocida A:3 and provides the foundations for further investigation of the pathogenesis of this disease in cattle.