Kinetics of humoral immune response in pigs vaccinated against foot and mouth disease (original) (raw)
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Testing the Antibody Response of Pigs to Foot-and- Mouth Disease Vaccines
2013
informational purposes. While APL has no reason to believe that the information contained in this publication is inaccurate, APL is unable to guarantee the accuracy of the information and, subject to any terms implied by law which cannot be excluded, accepts no responsibility for loss suffered as a result of any party’s reliance on the accuracy or currency of the content of this publication. The information contained in this publication should not be relied upon for any purpose, including as a substitute for professional advice. Nothing within the publication constitutes an express or implied warranty, or representation, with respect to the accuracy or currency of the publication, any future matter or as to the value of or demand for any good. Testing the Antibody Response of Pigs to Foot-andMouth Disease Vaccines
Journal of Biological Standardization, 1984
In trials performed in Brazil, England and Germany, 765 pigs were challenged at one, three to four or 17 .weeks after vaccination and their sera were titrated by macrocolour, microcolour or microcytopathic effects neutralization tests. Using Ol virus strains and challenge intervals of three to four weeks, significant correlations (P-~ 0-01) were demonstrated between protection and serum neutralizing titres although the titres corresponding to given levels of protection varied with the titration method employed. The protective capacity of antiserum was confirmed by passive antibody transfer experiments. Retrospective analysis of 49 challenge tests showed that when the macrocolour test was employed, a group mean (n = 8) serum titre of 1-74 log10 SNS0 corresponded with five out of eight (or more) protected from challenge in 92% of cases. The possibility of using serum titres as an alternative to challenge for oil emulsion vaccine potency tests is discussed.
Kinetics of immune response to foot-and-mouth disease virus (type Asia 1) in experimental cattle
Veterinary Research Communications, 2009
Humoral and mucosal (secretory antibody)immune response to FMDV type Asia 1 in cattle was analyzed after vaccination and infection using virus neutralizing test (VNT). Vaccination (1/16th the usual dose) failed to protect cattle from generalized clinical disease following experimental FMDV Asia 1 infection. Our results showed that infection induced higher and prolonged serum antibody titres indicating antigen mass is important for optimal immune response. Experimental FMDV infection induced significant secretory antibody (mucosal) response in cattle. Though, there was no difference in the serum antibody response between the cattle that developed generalized infection (unprotected) and those with only localized infection (protected), secretory antibody response differed, wherein the unprotected cattle had higher secretory response than protected cattle. Thus, FMDV Asia 1 infection stimulates a similar serum antibody response and a unique secretory antibody response among the infected cattle. Keywords Cattle . FMDV . Humoral immune response . Mucosal immune response . Neutralizing antibody titres . Secretory antibody response . Serum antibody response Abbreviations BHK 21 baby hamster kidney cell line clone 12 DPI days post infection FMD foot-and-mouth disease FMDV FMD virus ID 50 infective dose 50 ONF oro-nasal fluid Vet Res Commun (
Age-dependent immune response in pigs against foot-and-mouth disease virus in vitro
Journal of Animal Science and Technology
Competing interests No potential conflict of interest relevant to this article was reported. Funding sources State funding sources (grants, funding sources, equipment, and supplies). Include name and number of grant if available. This work was carried out with the support of the "Cooperative Research Program for Agriculture Science and Technology Development (Project title: Development of immune enhancement technology by investigating resistance factors for ASF and foot-and-mouth disease virus infection, Project No. PJ01491201)", Rural Development Administration, Republic of Korea Availability of data and material Upon reasonable request, the datasets of this study can be
IgA Antibody Response of Swine to Foot-and-Mouth Disease Virus Infection and Vaccination
Clinical and Vaccine Immunology, 2010
Foot-and-mouth disease virus (FMDV) continues to be a significant economic problem worldwide. Control of the disease involves the use of killed-virus vaccines, a control measure developed decades ago. After natural infection, the primary site of replication of FMDV is the pharyngeal area, suggesting that a mucosal immune response is the most effective. Humoral immunity to killed-virus vaccination induces antibodies that can prevent the clinical disease but not local infection. Determining whether infection or vaccination stimulates IgA-mediated local immunity depends on the method of analysis. Different assays have been described to analyze the quality of antibody responses of cattle and swine to FMDV, including indirect double-antibody sandwich enzyme-linked immunosorbent assay (IDAS-ELISA) and antibody capture assay-ELISA (ACA-ELISA). We tested these assays on swine and show that vaccinated animals had FMDV-specific IgM and IgG but no IgA in either serum or saliva. After the infec...
Vaccine, 2004
The objective of this study was to investigate whether and at what time interval could vaccination reduce transmission of foot-and-mouth disease virus (FMDV) among pigs. Reduction of virus transmission by vaccination was determined experimentally. Transmission of FMDV was studied in three groups of ten pigs: one non-vaccinated group and two groups that were vaccinated 7 days (−7 dpi) and 14 days before inoculation (−14 dpi), respectively. Five randomly selected pigs from each group were inoculated with FMDV type O Taiwan, while the other five pigs left in the groups were exposed to the inoculated pigs by direct contact. Clinical signs were recorded, virus isolation and RT-PCR were carried out on oropharyngeal fluid (OPF), and the neutralizing antibody titres and the antibody response against non-structural (NS) proteins of FMDV were determined. No virus transmission was observed in the −14 dpi group, whereas virus transmission was observed in all contact pigs affecting both the non-vaccinated and the −7 dpi group. The reproduction ratio R in the −14 dpi vaccinated group was significantly lower than that of the non-vaccinated group. This study confirms the potential of vaccination as an important tool to reduce transmission of FMDV.
Journal of Veterinary Medical Science, 2011
The presence of serum antibodies for nonstructural proteins of the foot-and-mouth disease virus (FMDV) can differentiate FMDV-infected animals from vaccinated animals. In this study, a sandwich ELISA was developed for rapid detection of the foot-andmouth disease (FMD) antibodies; it was based on an Escherichia coli-expressed, highly conserved region of the 3ABC nonstructural protein of the FMDV O/TW/99 strain and a monoclonal antibody derived from the expressed protein. The diagnostic sensitivity of the assay was 98.4%, and the diagnostic specificity was 100% for naïve and vaccinated pigs; the detection ability of the assay was comparable those of the PrioCHECK and UBI kits. There was 97.5, 93.4 and 66.6% agreement between the results obtained from our ELISA and those obtained from the PrioCHECK, UBI and CHEKIT kits, respectively. The kappa statistics were 0.95, 0.87 and 0.37, respectively. Moreover, antibodies for nonstructural proteins of the serotypes A, C, Asia 1, SAT 1, SAT 2 and SAT 3 were also detected in bovine sera. Furthermore, the absence of cross-reactions generated by different antibody titers against the swine vesicular disease virus and vesicular stomatitis virus (VSV) was also highlighted in this assay's specificity.
Humoral immune response induced by various foot and mouth disease vaccines in buffalo calves
Pakistan Veterinary Journal, 2015
Received: Revised: Accepted: March 30, 2014 February 10, 2015 February 21, 2015 Humoral immune response to 5 FMD vaccines (3 imported and 2 locally manufactured) available in Pakistan was studied in 90 buffalo calves over seven months period. All vaccines were trivalent and contained serotypes A, O and Asia1. Sera were analyzed for the presence of antibodies against non-structural proteins (NSP) and structural proteins (SP) using commercially available 3ABC-trapping indirect ELISA and liquid phase blocking ELISA, respectively. Maternal NSP antibodies waned by 5 month of age in most of the buffalo calves. Vaccine induced NSP antibodies were seen in only two animals, both vaccinated with locally manufactured unpurified vaccines. All three imported vaccines induced significantly higher titers against SP than local vaccines. These titers seemed to stay at protective level for almost six months in two imported vaccines which had aluminum hydroxide and saponin as adjuvant. Peak titers of ...
Journal of Veterinary Medicine Series B-infectious Diseases and Veterinary Public Health, 1988
The duration of immunity was evaluated for a period of seven months in pigs vaccinated with a monovalent foot and mouth disease vaccine inactivated with formaline and bromoethylenimine, combined with an oil adjuvant. A satisfactory degree of immunity was recorded at one month post-vaccination and the antibody levels were substained for the period under study. The vaccine also induced a good protection to the challenge even after seven months following a single administration of the vaccine.
2007
Introduction: Serological diagnosis of foot-and-mouth disease (FMD) was investigated in Hong Kong where pig herds are known to be infected periodically with serotype O and preventive vaccination is widely used. The aim of the study was to identify herds containing either vaccinated or vaccinated and infected pigs and to collect blood samples to evaluate the specificity and sensitivity of tests for detection of antibodies to nonstructural viral proteins (NSP) that are designed to identify infected animals within a vaccinated population.