Streptomyces peucetius M1 and Streptomyces lavendulae M3 Soil Isolates as a Promising Source for Antimicrobials Discovery (original) (raw)
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The Antibacterial Activities of Secondary Metabolites Derived from Streptomyces sp
Progress in Microbes and Molecular Biology, 2022
The spreading of infectious diseases caused by the emergence of Multidrug-Resistant (MDR) pathogens is a global threat that has led to numerous deaths annually. In view of this, there is an overwhelming need to discover new bioactive compounds with effective antimicrobial properties. Concurrently, the genus Streptomyces has a growing reputation as a potential biological source of various antibiotics and other bioactive metabolites. Streptomyces sp. has been isolated from different sources, including terrestrial and marine habitats with a myriad of promising compounds that could be used to treat MDR pathogens. Therefore, this study presents a systematic review of the antibacterial activities of Streptomyces-derived secondary metabolites. The Preferred Reporting Items for Systematic Reviews and Meta-Analyses (PRISMA) guidelines and checklist were employed in this study to collect relevant articles from two research databases, namely PubMed and Science Direct. The selection process includes identification, screening, eligibility, and inclusion of articles. Several keywords and criteria were established for the screening and selection process. Based on the results, a total of 26 articles were selected from 70 potential articles. The articles were published between 2015 and 2020 with most studies being published in 2020, indicating an increased interest in Streptomyces and its derived compounds. Approximately 51 different Streptomyces-derived compounds have been identified, ranging from crude extracts, pure PMMB 2022, 5, 1; a0000281 2 of 25 compounds, and partially purified compounds. Various parameters were also used to assess their antibacterial activities, particularly the Minimum Inhibitory Concentration (MIC) (69%) and the zone of inhibition (11%). Moreover, the antibacterial activities of these compounds were effective on numerous gram-positive and gram-negative bacteria. Furthermore, 46% and 54% of the selected studies were focused on inhibiting MDR and non-MDR pathogens, respectively. In conclusion, both crude and purified compounds from Streptomyces sp. exhibited strong antibacterial effects. It is expected that extensive future research would develop a standard method to compare the antibacterial strength of each extracted compound from Streptomyces sp. and determine the most effective bioactive compounds to treat diseases caused by MDR pathogens.
Frontiers in Cellular and Infection Microbiology
BackgroundAntimicrobial resistance is a serious threat to public health globally. It is a slower-moving pandemic than COVID-19, so we are fast running out of treatment options.PurposeThus, this study was designed to search for an alternative biomaterial with broad-spectrum activity for the treatment of multidrug-resistant (MDR) bacterial and fungal pathogen-related infections.MethodsWe isolated Streptomyces species from soil samples and identified the most active strains with antimicrobial activity. The culture filtrates of active species were purified, and the bioactive metabolite extracts were identified by thin-layer chromatography (TLC), preparative high-performance liquid chromatography (HPLC), nuclear magnetic resonance (NMR) spectroscopy, and gas chromatography-mass spectrometry (GC-MS). The minimum inhibitory concentrations (MICs) of the bioactive metabolites against MDR bacteria and fungi were determined using the broth microdilution method.ResultsPreliminary screening reve...
Mycobiology, 2009
A new strain of Streptomyces identified as Streptomyces rajshahiensis (RUPA08-PR) was isolated from the soil of Rajshahi, Bangladesh. Ethyl acetate was the most convenient solvent for the extraction of antimicrobial metabolites from the culture filtrate of this strain. From the ethyl acetate extract compound 1 was isolated, purified and characterized by spectral data. The compound showed antibacterial activity against a number of both Gram positive and Gram negative bacteria but did not have antifungal activity. The minimum inhibitory concentration (MIC) of the compound 1 and ethyl acetate extract was determined against four Gram positive and four Gram negative bacteria and the values were found between 32 and 128 mg/ml. Both the isolated compound 1 and the ethyl acetate extract exhibited cytotoxic effect in brine shrimp lethality bio-assay with LC 50 values of 1.32mg/ml and 0.89mg/ml respectively.
Anti-cancer and antimicrobial potential of five soil Streptomycetes : a metabolomics-based study
Royal Society Open Science, 2022
Lack of new anti-cancer and anti-infective agents directed the pharmaceutical research to natural products' discovery especially from actinomycetes as one of the major sources of bioactive compounds. Metabolomics- and dereplication-guided approach has been used successfully in chemical profiling of bioactive actinomycetes. We aimed to study the metabolomic profile of five bioactive actinomycetes to investigate the interesting metabolites responsible for their antimicrobial and anti-cancer activities. Three actinomycetes, namely, Streptomyces sp. SH8, SH10 and SH13, were found to exhibit broad spectrum of antimicrobial activities, whereas isolate SH4 showed the broadest antimicrobial activity against all tested strains. In addition, isolates SH8, SH10 and SH12 displayed potent cytotoxicity against the breast cancer cell line Michigan Cancer Foundation-7 (MCF-7), whereas isolates SH4 and SH12 exhibited potent anti-cancer activity against the hepatoma cell line hepatoma G2 (HepG2) ...
Streptomyces: Still the Biggest Producer of New Natural Secondary Metabolites, A Current Perspective
Microbiology Research
There is a real consensus that new antibiotics are urgently needed and are the best chance for combating antibiotic resistance. The phylum Actinobacteria is one of the main producers of new antibiotics, with a recent paradigm shift whereby rare actinomycetes have been increasingly targeted as a source of new secondary metabolites for the discovery of new antibiotics. However, this review shows that the genus Streptomyces is still the largest current producer of new and innovative secondary metabolites. Between January 2015 and December 2020, a significantly high number of novel Streptomyces spp. have been isolated from different environments, including extreme environments, symbionts, terrestrial soils, sediments and also from marine environments, mainly from marine invertebrates and marine sediments. This review highlights 135 new species of Streptomyces during this 6-year period with 108 new species of Streptomyces from the terrestrial environment and 27 new species from marine so...
Journal of Scientific Research, 2009
A new actinomycete strain was isolated from Western part of Bangladesh and identified as a new Streptomyces species on the basis of its morphological, biochemical, cultural characteristics and 16S rRNA data. The present paper describes the isolation and characterization of compound 1 from this new Streptomyces species with the help of various chemical and spectroscopic methods. Antimicrobial activity of compound 1 was tested by disc diffusion assay method and compared with that of standard antibiotics (Kanamycin for antibacterial activity and Nystatin for antifungal activity). The compound has been found to exhibit moderate to strong antimicrobial activity against the test organisms. Cytotoxicity of the compound 1 and the pet. ether extract of Czapek Dox (alkaline) broth of Streptomyces species was evaluated in brine shrimp bioassay with LC 50 values of 23.85 µg/ml (ppm) and 19.95 µg/ml (ppm), respectively.
Streptomyces Secondary Metabolites
Basic Biology and Applications of Actinobacteria, 2018
Actinobacteria are found spread widely in nature and particular attention is given to their role in the production of various bioactive secondary metabolites. Tests on soil samples show that there can be a diversity of actinomycetes depending on the climate, the area it is growing in, how dry the soil is, and the quality of the soil. However, it was agreed after tests in Yunnan, China, that the genus Streptomyces sp. is most important in ecological function, representing up to 90% of all soil actinomycetes, and therefore helping to show the important characteristics needed of the soil actinomycete population. Streptomycete compounds are used for other biological activities, not just for antibiotics. It has been found that metabolites can be broadly divided into four classes: (1) regulatory activities in compounds, these include consideration of growth factors, morphogenic agents and siderophores, and plants promoting rhizobia; (2) antagonistic agents, these include antiprotozoans, antibacterials, antifungals, as well as antivirals; (3) agrobiologicals, these include insecticides, pesticides, and herbicides; and (4) pharmacological agents, these include neurological agents, immunomodulators, antitumorals, and enzyme inhibitors. It is found that Streptomyces hygroscopicus is one of the very best examples because it secretes in excess of 180 secondary metabolites to locate simultaneous bioactivities for a given compound. Increasingly, both its agricultural and pharmacological screenings are being used in conjunction with antimicrobial tests and have revealed several unusual aerobiological and therapeutic agents, which were hitherto unknown for biological use as antibiotics. Since streptomycetes are now being used increasingly to screen for antimicrobial activity, reports show the existence of secondary metabolites with other activities that may have been missed. Currently, nearly 17% of biologically active secondary metabolites (nearly 7600 out of 43,000) are known from streptomycetes. It has been found that soil streptomycetes are the main source used by bioactive secondary metabolites. However, recently there have been many and varied types of structurally unique and biologically active secondary metabolites found and obtained from marine actinomycetes, including those from the genus Streptomyces. Also, compounds that are synthesized by streptomycetes exhibit extreme chemical diversity. Diverse form made from from simple amino acid
This study was designed to isolate and evaluate the antimicrobial potential of soil Actinomycetes. The secondary metabolites of actinomycetes are the prominent source of antibiotics. In the present study, 13 actinomycetes strains were isolated from the Karachi soil. Primary antagonistic screening was performed by cross streak and over lay method against Gram positive and Gram negative bacterial strains. Out of 13 isolates, 8 isolates inhibited the tested strains growth. Among them 31% isolates inhibited Gram positive bacteria, 23% isolates were active against Gram positive and negative strains, while Gram negative growth was inhibited by 8% isolates. These eight strains were than subjected to secondary screening by submerged fermentation. The antimicrobial activity of fermented broth was evaluated by agar well diffusion method. Based on the antagonistic spectrum, the strain GZ024 was selected for further study. The isolate produced Retinaculum sporangium with oval shaped spores (with marty surface), arranged in clusters, citrate, catalase and gelatinase positive, produced acidic butt and alkaline slope on TSI and only ferment the glucose hence named Streptomyces sp. GZ024. The growth conditions were optimized: the strain grow well at 25˚C, pH 9 and 3% NaCl concentration. The minimum inhibitory concentration of antimicrobial substance of Streptomyces sp. GZ024 was found to be 1:8. Ethyl acetate solvent was proved to best solvent for the antibiotic metabolite extraction. The extract was stable at 100˚C and pH 9. The organism starts producing antibiotics at log phase and showed maximum activity when organism entered in stationary phase.
Brazilian Journal of Microbiology, 2011
An indigenous Streptomyces isolate CTF9, exhibiting promising antifungal activity against Mucor miehei and Candida albicans in pre-screening studies, was investigated by cultivation in a 50-L fermenter and by subsequent isolation, purification, and structure elucidation of the active metabolites. Based on the morphological, biochemical, and physiological characterization, as well as the 16S rRNA gene sequence, the isolate CTF9 was identified as Streptomyces malachitofuscus. Using a series of chromatographic techniques, two pure compounds were isolated from the obtained extracts after the fermentation of the isolate CTF9. The isolated compounds were identified as phenylacetic acid and indolyl-3-lactic acid by mass spectrometry (MS) and NMR analysis. The culture optimization studies revealed that the isolate CTF9 can use a variety of low-cost carbon and nitrogen sources to generate the maximum quantity of industrially important metabolites at an elevated temperature of 35°C and at a pH 7.8. Sajid, I. et al. Antifungal metabolites from the Streptomyces water, deciduous forest, tropical forest, wasteland, and cave soils 29). Their natural habitat soil is nutritionally, biologically and physically complex and variable, demanding their fast adaptation. As a consequence, they are able to perform a broad range of metabolic processes and to produce an immense diversity of bioactive secondary metabolites.
International Journal of Agriculture and Biology
This research is concerned with the isolation, purification and partial characterization of two metabolites from culture filtrate of Streptomyces sp. KGG32, by solvent extraction, silica gel chromatography, UV and FT-IR spectroscopic analyses. This strain is a highly stable antimicrobial metabolite producer against different microorganisms such as S. aureus, B. cereus, B. subtilis, Kocuria rhizophila, E. coli, Enterococcus faecalis and C. albicans under in-vitro growth conditions. In order to obtain crude extract, ten liters of the fermentation broth was extracted with different solvents and concentrated until dryness. The crude extract was subjected to silica gel column chromatography and the fractions showing the highest antimicrobial activity were pooled and determined by TLC for purity. Extraction of fermentation broth, various separation and purification steps led to isolation of two pure active compounds having a retention factor of 0.22 (methanol: water, 3:1, v/v) and 0.64 (e...