Comparison of the Percentages of Peripheral Blood CD4+ CD25+ T Lymphocytes in Recurrent Abortion and Normal Pregnancy (original) (raw)
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Reproductive immunology: laboratory tests for analysis of recurrent spontaneous abortion
Clinical Immunology Newsletter, 1995
Beer AE, Semprini AE, Zhu X, Quebbemann JF: Pregnancy outcome in human couples with recurrent spontaneous abortions: HLA antigen profiles HLA antigen sharing female MLR blocking factors and paternal leukocyte immunization. Exp Clin Immunogenetics 2:137-142. 1985. Branch DW. Rote NS, Dostal DA, Scott RA: Association of lupus anticoagulant with antibody against phosphatidylserine. Clin Immunol Immu-nopathol42:63-75, 1987. Coulam CB: Alternative treatment to lymphocyte immunization for treatment of recurrent spontaneous abortion-Immunotherapy with intravenous immunoglobulin for treatment of recurrent pregnancy loss: American Experience. Am J Repmd Immunol 32:286-289, 1994. Coulam CB. Clark DA, Collins J, Scott JR, Schlesselman JS: Worldwide collaborative observational study and meta-analysis on allogenic leukocyte immunotherapy for recurrent spontaneous abortion. Am J Reprod Immunol32:55-72, 1994. Cross JC, Werh Z, Fisher SJ: Implantation and the placenta: Key pieces of the development puzzle. Science 266:1508-1518, 1994. Ellis SA, Palmer MS, McMicbae.1 AJ: Human tmphoblasts and the choriocarcinoma cell line BeWo express a truncated HLA class I molecule. J Immunol 144:731, 1990. Gill TJ III: Immunogenetics of spontaneous abortions in humans Transplantation 35:1-4, 1983. Gilman-Sachs A, Lubinski J, Beer AE, BrendS, Beaman KD: Patterns of anti-phospholipid antibody specificities. J Clin Lab Immunol35:83-88, 1991. Gilman-Sachs A, Luo S-Y, BeerPAE, Beaman KD: Analysis of anti-lymphocyte antibodies by flow cytometry or micmlymphocyto~oxicity in women with recurrenl spontaneous abortions immunized with paternal leukocytes. J Clin Lab lmmunol 30:53-59, 1989. Hunt JS, Orr HT: HLA and maternal-fetal recognition FASEB 6~2344-2348, 1992. Jokhi PP. King A, Sharkey M, Smith SK, Loke Y: Screening for cytokine messenger ribonucleic acids in purified human decidual lymphocyte populations by the reverse-transcriptase polymerase chain reaction. J Immunol 153:4427-4435, 1994.
Human Reproduction, 2001
A number of cases of unexplained (idiopathic) recurrent spontaneous abortions may be attributable to immunological mechanisms. Several lines of evidence indicate that some immunocompetent effector cell populations play an important role in the pathogenesis of unexplained miscarriages. However a suitable method is lacking for defining an existing immunological background of recurrent spontaneous abortions. We tried to find a useful cellular immunological method, that is suitable for predicting the eventual immunological cause in the case of unexplained recurrent spontaneous abortions. We have examined the anti-paternal cytotoxic T-lymphocyte precursor frequencies by cell-mediated lympholysis and limiting dilution analysis in the peripheral blood of women with recurrent spontaneous abortions in order to reveal the functional role of this cell population in spontaneous abortions. An extremely high partner allo-antigen-specific cytotoxic T-lymphocyte precursor frequency was determined in the case of all those habitual aborters, where no other than an immunological cause could be responsible for the abortions. This phenomenon supports the important role of the T-lymphocytes in this disorder. We suggest that the immunological background of recurrent spontaneous miscarriages might be determined on the basis of a very high cytotoxic T-lymphocyte precursor frequency. This diagnostic test might be useful in selecting patients for immunotherapy.
Immunological evaluation of patients with recurrent abortion
Journal of Reproductive Immunology, 2002
In a prospective study, we performed immunological tests in patients with recurrent abortion. Nine couples with two or more fetal losses of no apparent cause were selected as the patient group, and nine volunteer couples with at least two children and without a history of abortion were used as controls. The frequency of major histocompatibility complex (human leukocyte antigens, HLA) antigen sharing was determined by serological methods, antipaternal antibodies by microlymphocytotoxicity, lymphocyte phenotypes (CD4, CD8, CD19, CD16, CD56 and HLA-DR positive cells) by flow cytometry and natural killer (NK) cytotoxicity by 51 Cr release. NK activity was correlated to the degree of HLA-C sharing and to the percentage of CD16 + and CD56 + cells and to progesterone levels measured by radioimmunoassay. No difference in class I or class II HLA antigen sharing was detected between couples with and without recurrent abortion. Antipaternal antibodies were not found in the serum of any woman of the study. A higher absolute number of CD8+ cells (P=0.01) and a trend to increased CD19 + cells (P= 0.05) were observed among patients. NK activity did not differ between the two groups when expressed as specific cytotoxicity and it was reduced among patients with recurrent abortion when expressed as lytic units/10 7 cells (P =0.04).
PLOS ONE, 2015
The development of pregnancy is possible due to initiation of immune response in the body of the mother resulting in immune tolerance. Miscarriage may be caused by the impaired maternal immune response to paternal alloantigens located on the surface of trophoblast and fetal cells. The aim of the study was to compare the population of circulating dendritic cells (DCs) and CD4+CD25+Foxp3+ regulatory T cells (TREGs) in the first trimester of a normal pregnancy and in women with recurrent miscarriage and an attempt to determine the relationship between these cells and the role they may play in human reproductive failures. The study was conducted in a group of 33 first trimester pregnant women with recurrent miscarriage and in a group of 20 healthy pregnant women in the first trimester of normal pregnancy. Among mononuclear cells isolated from peripheral blood, the populations of DCs and TREGs were assessed by flow cytometry. The percentage of myeloid DCs and lymphoid DCs showed no significant difference between study and control group. Older maternal age and obesity significantly reduced the pool of circulating myeloid and lymphoid DCs (R=-0.39, p=0.02). In miscarriages the percentage of circulating TREGs was significantly lower compared to normal pregnancies (p=0.003). Among the analysed factors the percentage of TREGs was the most sensitive and the most specific parameter which correlated with the pregnancy loss. The reduction in the population of circulating TREGs suggests immunoregulatory mechanisms disorder in a pregnancy complicated by miscarriage.
PURPOSE: To evaluate the levels of peripheral blood CD34+ cells in women who subsequently had a spontaneous miscarriage (SM). MATERIALS AND METHODS: We enrolled 11 women who had SM, matching them for age, BMI and gestational age with 33 healthy pregnancies (controls). From a blood sample at 9th-11th weeks of pregnancy, we evaluated PAPP-A, free β-hCG, T (suppressor and helper), NK, B, CD34+ cells. RESULTS: In peripheral blood of women who had SM, PAPP-A and CD34+ cells were significantly lower (p < 0.001) compared to control group. CONCLUSIONS: CD34+ cell low level in peripheral blood is associated with increased risk of SM.
2021
BackgroundAppearance of improper immune responses against the fetus and/or inadequate immunoregulatory mechanisms during pregnancy may lead to recurrent spontaneous abortion (RSA). TH17 cells play a significant role in inducing inflammation, autoimmune disease, and acute transplant rejection, while regulatory T (Treg) cells moderate the function of immune system in order to retain homeostasis.MethodsThis case-control study was designed to evaluate TH17 as well as Treg cells in 25 women with RSA and 25 age-matched healthy non-pregnant women. Flow cytometric assay was performed using monoclonal antibodies to detect CD4+CD25+ Treg cells (CD25dim and CD25bright). FoxP3 and RORγt expressions were compared using real-time PCR, and pro-inflammatory and anti-inflammatory cytokines were measured by ELISA kits. Independent-samples T test was employed for statistical analysis. ResultsThe ratio of CD4+CD25bright T cells was remarkably lower in women with RSA (P<0.05), and CD4+CD25dim T cells...