Special research presentation: isolation and characterization of cardiac specific endothelium in murine development and human embryonic stem cell differentiation (original) (raw)

2014, Fertility and Sterility

identify failed probes, and the methylation level of each CpG locus (beta value) was calculated in GenomeStudio Methylation module. Possible plate/batch effect was assessed visually by Principal Component Analysis, and data was normalized using the Chip Analysis Methylation Pipeline (ChAMP) package (implemented in R). RESULTS: Genomic DNAs isolated from these CVS samples successfully underwent bisulfite conversion. All samples had >99.9% call rate, and two independent technical replicates were clustered tightly. Differentially methylated loci are currently being analyzed in Ingenuity Pathway Analysis (IPA) to identify pathways that may be modified by IVF or non-IVF fertility treatments. CONCLUSION: These results indicate that CVS samples from our Prenatal Biorepository that were preserved in RNAlater can be used for successful isolation of high quality genomic DNA, and that this DNA is suitable for use in epigenetic arrays. Further functional analysis is undergoing, and our findings will determine whether there are epigenetic differences between the groups that may affect placentation and fetal development.

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