Cardiogenic differentiation and in vivo tracking of porcine endometrial stromal mesenchymal stem cells (original) (raw)

Abstract

Porcine mesenchymal stem cells (MSCs) are of great interest due to their potential to differentiate into multilineages as a good candidate for regenerative medicine (Rho et al., 2009). Porcine uteri were obtained from slaughter house (n=5) and endometrial stromal cells were isolated and plated 500 cells/cm3 in ADMEM containing 10% FBS to obtain purified single suspensions of stromal cells. Isolated stromal cells were analyzed for mesenchymal markers, pluripotent genes, cardiogenic differentiation, and finally homing of in vivo injected cells. The isolated cells were positive for mesenchymal stem cell markers CD29, CD44, CD90, CD105, CD140b, and CD144, negative for epithelial marker CD9, hematopoietic markers CD34 and CD45 analyzed by flow cytometry and Semi-Quantitative PCR. Pluripotent markers OCT4, SOX2, and NANOG were positively expressed by western blotting and semi-Quantitative PCR, proving they were stem cell in origin rather than fibroblast (Li H Y et al., 2010). Further, the...

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