Seroprevalence of camel brucellosis in Qatar (original) (raw)
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2021
A study was done to investigate the incidence of Brucella abortus in Camels in different districts of South Punjab. Brucellosis is a disease that affects a variety of animals, including humans, and it is more severe in humans than in animals. Because of its zoonotic nature, it poses a significant threat to human health. In this study, we tested n = 170 sera samples and n = 60 milk samples from camels in four districts of Pakistan's South Punjab province. Different diagnostic test was used to investigate the efficacy of detecting Brucella in the blood of infected animals after serum samples were proven to be positive for B. abortus by serology. Serological techniques such as the c-ELISA, Serum Agglutination Test, and Rose Bengal Plate Test were used to diagnose Brucella abortus. The screening tests employed were the Rose Bengal Plate Test, serum agglutination test, and competitive-ELISA (c-ELISA), and the overall prevalence of brucellosis was found to be 18.24%, 18.24%, and 12.94...
Seroprevalence and Molecular Identification of Brucella spp. in Camels in Egypt
Microorganisms
Brucellosis is one of the most important worldwide zoonoses of many countries including Egypt. Camel brucellosis has not gained much attention in Egypt yet. This study is focused on the three governorates with the highest camel populations and the largest camel markets in the country to determine the disease seroprevalence and identify the Brucella species in local camel holdings. In total, 381 serum samples were collected from male and female camels from Giza, Aswan, and Al-Bahr Al-Ahmar (the Red Sea) governorates. Samples were serologically examined using the Rose–Bengal plate test (RBPT), indirect ELISA (i-ELISA), competitive ELISA (c-ELISA) and complement fixation test (CFT). Brucella antibodies were detected in 59 (15.5%), 87 (22.8%), 77 (20.2%) and 118 (31.0%) of sera by RBPT, i-ELISA, c-ELISA and CFT, respectively. Using real-time PCR, Brucella DNA was amplified in 32 (8.4%) seropositive samples including Brucella abortus (25/32), Brucella suis (5/32) and Brucella melitensis ...
Sero-Prevalence of Anti-Brucella Antibodies in Camels in El-Gedarif State: A Short Communication
2017
DOI: 10.21276/sjavs.2017.4.11.4 Abstract: The aim of this study was to determine seroprevalence of anti-brucella antibodies in camels in three different localities in El-Gedarif state, Eastern Sudan. Two hundred serum samples were collected from adult male and female camels were examined using the rose Bengal plate test (RBPT) and serum agglutination test (SAT). Overall seroprevalences of 32% (n= 64, 95% CI ±6.46) and 23% (n= 46, 95% CI ±5.83) were reported by RBPT and SAT respectively. There were no significant statistical differences in the reported seroprevalences among localities. Furthermore, no brucella species was isolated from any of the cultured lymph node samples. This study showed that the seroprevalence of anti-brucella antibodies using RBPT and SAT is relatively high. Further studies investigating brucellosis across the whole state should be carried out to explore the epidemiology of this disease in camels.
Serological Screening of Brucellosis in One-Humped Camels, Iraq
IAR Consortium, 2022
Background: Camel brucellosis is one of the most neglected diseases due to thoughts that camel is either exposed rarely to Brucella spp. or it more resistance to infection. Therefore, the current study aimed for sero-investigation of brucellosis in camels using three diagnostic assays; RBT, STA and ELISA. Materials and methods: Totally, 200 camels of different ages and sexes existed in Wasit province (Iraq) were subjected to collection of jugular venous blood sampling under aseptic conditions from January to June (2022). Results: There were 2.5%, 6.5%, and 18.5% positive camels by RBT, STA and ELISA, respectively. Relationship between the findings of applied assays revealed that 2.5% samples w e r e positives by both ELISA and RB, 16% by ELISA; while, no positive samples by the RB only (0 %). Also, there were 5.5% positive samples by both ELISA and STA, 1% by STA only, and 13% positives by ELISA only. Distribution of positive results among sex and age factors was differed significantly. Concerning sex, positive results reported in males by S T A a n d ELISA were 2.44% and 12.2%, respectively; while in females, t her e wer e 3.15%, 7.55% and 13.22% positive camels by RB, STA, and ELISA, respectively. Regarding age, positive results of animals aged 1-4 years were 1.35%, 4.05%, and 10.81%; whereas in those aged 4 years, there were 3.18%, 7.94%, and 23.02% by RB, STA and ELISA, respectively. Conclusion: The prevalence of camel brucellosis was higher than expected, and ELISA assay was revealed a high reliability in detection of infection. Furthermore studies in other areas in Iraq are necessary to confirm the real prevalence of camel brucellosis. As well as, the role of camels in transmission of the disease to other domestic animals or even humans should be aimed in other carried studies.
Camels still play an important role in providing food security in several developing countries, especially in the arid zones. As brucellosis has zoonotic importance, thereby diseased camels can infect humans chiefly via milk. In spite of its vital importance, limited studies are available of brucellosis in camels in Pakistan. Keeping in view the fact, this study was designed and executed. For this purpose, serum samples (n=761) from various herds of camel with history of abortion from different locations of Punjab province, Pakistan were collected. Initial screening was done using Rose Bengal plate test (RBPT). To avoid improper judgment of the diagnosis of brucellosis caused by serological tests, positive samples were subjected to competitive enzyme-linked immunosorbent assay (cELISA). Detection of Brucella genome was carried out through conventional PCR in RBPT and cELISA positive test samples. Overall, 3.41% (n=26, 95% CI 2.24-4.97) of the camels were seropositive with RBPT. Prevalence of brucellosis significantly varied in the animals with different geographical source, sex, age, lactation number and health status. In molecular analysis, out of 18 samples confirmed through cELISA, only two samples were positive for Brucella genome through PCR. The sequences were aligned with reported sequences in NCBI GenBank and revealed 100% sequence homology with the bcsp-31 gene of Brucella reported from other parts of the world. The study highlights the epidemiologic, economic and public health impact of camel brucellosis as a basis for designing further diagnostic improvements and effective control strategies.
Risk factors associated with camel brucellosis in Jordan
Tropical Animal Health and Production, 2008
During the period between February, 2004 and December, 2006, a cross-sectional study was performed to investigate some epidemiological aspects related to camel brucellosis in Jordan. Four hundred twelve camel sera from 37 herds were randomly collected and analyzed using Rose Bengal plate test and complement fixation test. A structured pre-tested questionnaire was administered to collect information on camel herd health and management. A multivariable logistic regression model was constructed to investigate risk factors associated with seropositivity to Brucella antigens. Moreover, the incidence of Brucella-specific abortion was investigated in 7 camel herds located in different locations in Southern Jordan. The true prevalence of Brucella-seropositive in camels was 12.1%. Thirteen (35.1%) herds had at least one positive camel. The seroprevalence of brucellosis in camels was significantly higher in the southern part of Jordan than that in central or northern Jordan. The multivariable logistic regression model on both individual and herd levels revealed large herds and contact with small ruminants as risk factors for Brucella seropositivity. On the other hand, using disinfectants was identified as a protective factor (OR = 0.8; 95% CI: 0.1, 0.9) only on the camel herd level. The incidence of Brucella-caused abortion was 1.9%. Brucella melitensis biotype 3 was isolated from 4 aborted camel fetuses.
Egypt imports camels from east Africa to compensate for the gap in meat production. Some imported camels are kept for breeding as scattered populations countrywide without being ear-tagged. The seroprevalence of brucellosis among camels in the source countries is an indicator for potential transboundary brucellosis being introduced to Egypt through untested camels. Brucella antibody profile was studied in male one-humped camels from Somaliland and Eastern Ethiopia quarantined in Berbera for intended export to the Arabian Gulf States for meat consumption. A total of 3,200 camels were screened for brucellosis by the modified Rose-Bengal plate test, where 103 failed the test. The apparent prevalence was 2.9375% and the estimated true prevalence was 0.445%. These samples were further tested using the buffered acidified plate antigen (BAPA) test, brucellosis card test (BCT), microplate agglutination test with and without EDTA and mercaptoethanol, rivanol-precipitation plate agglutination test (Riv.T) and competitive ELISA (C-ELISA). Complement fixation was used as a gold standard in lieu of bacteriologic examination. Statistical analyses including analysis of variance followed by post hoc test, receiver operating characteristics curves, kappa agreement and diagnostic performance metrics, viz. sensitivity, specificity, false positive/ negative rates, positive/ negative predictive values, likelihood ratios of positive and negative results and diagnostic odds ratio were used. Results were fully discussed. It was concluded that the serologic profile had a dominating IgG subclass of antibodies due to 1 active infection. BAPA and modified BCT performed really well as binary screening markers. The performance indicators suggested Riv.T as an excellent candidate for simple and rapid disease confirmation. C-ELISA needs further adaptation to boost its performance in camels.
Serological and Molecular Screening of Camels for Brucellosis in Bikaner, Rajasthan, India
Current Journal of Applied Science and Technology
Brucellosis is an important zoonotic disease affecting domestic animals and humans worldwide. The present study was undertaken on camels in and around Bikaner city of Rajasthan state of India to assess the extent of prevalence of Brucellosis in camels in this region. Since Rose Bengal Plate test (RBPT) is a serological screening method for diagnosis of Brucellosis approved by the Office International des Epizooties (OIE), RBPT was employed for detecting antibodies against Brucella organisms in camels. Polymerase Chain Reaction is widely followed for molecular diagnosis of several infectious diseases. DNA from whole blood of camels was analyzed by PCR for detection of Brucella organisms in the blood of camels. Blood samples from 177 camels (108 males and 69 females) from Bikaner and nearby villages were analyzed for Brucellosis by RBPT. Fifteen camels [7 (46.66%) males and 8 (53.33%) females] were found positive. However, none of the DNA samples from whole blood (RBPT positive or neg...