Bulgarian Propolis Induces Apoptosis in Lymphoblast Cell Line L5178Y (original) (raw)
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Research Article Anticancer Activity of Indian Stingless Bee Propolis: An In Vitro Study
Anticancer activity of Indian Stingless bee propolis: An in vitro study, 2013
Indian stingless bee propolis has a complex chemical nature and is reported to possess various medicinal properties. In the present study, anticancer activity of the ethanolic extract of propolis (EEP) was explored by testing the cytotoxic and apoptotic effect in four different cancer cell lines, namely, MCF-7 (human breast cancer), HT-29 (human colon adenocarcinoma), Caco-2 (human epithelial colorectal adenocarcinoma), and B16F1 (murine melanoma), at different concentrations. Cytotoxicity was evaluated by MTT assay and Trypan blue dye exclusion assay. EEP at a concentration of 250 𝜇g/mL exhibited ≥50% mortality in all cell lines tested (i.e., IC50 value). EEP revealed a concentration and time dependent cytotoxic effect. Apoptosis was estimated by differential staining (ethidium bromide/acridine orange) and TUNEL (deoxynucleotidyl transferase-dUTP nick end labeling) assay. Light microscopy and atomic force microscopy demonstrated morphological features of apoptosis in all the cell lines after treatment with 250 𝜇g/mL EEP for 24 h. Thus, early onset of apoptosis is the reason for anticancer activity of Indian stingless bee propolis. Further, the antioxidant potential of Indian stingless bee propolis was demonstrated to substantiate its anticancer activity.
Anticancer Activity of Indian Stingless Bee Propolis: An In Vitro Study
Evidence-Based Complementary and Alternative Medicine, 2013
Indian stingless bee propolis has a complex chemical nature and is reported to possess various medicinal properties. In the present study, anticancer activity of the ethanolic extract of propolis (EEP) was explored by testing the cytotoxic and apoptotic effect in four different cancer cell lines, namely, MCF-7 (human breast cancer), HT-29 (human colon adenocarcinoma), Caco-2 (human epithelial colorectal adenocarcinoma), and B16F1 (murine melanoma), at different concentrations. Cytotoxicity was evaluated by MTT assay and Trypan blue dye exclusion assay. EEP at a concentration of 250 g/mL exhibited ≥50% mortality in all cell lines tested (i.e., IC 50 value). EEP revealed a concentration and time dependent cytotoxic effect. Apoptosis was estimated by differential staining (ethidium bromide/acridine orange) and TUNEL (deoxynucleotidyl transferase-dUTP nick end labeling) assay. Light microscopy and atomic force microscopy demonstrated morphological features of apoptosis in all the cell lines after treatment with 250 g/mL EEP for 24 h. Thus, early onset of apoptosis is the reason for anticancer activity of Indian stingless bee propolis. Further, the antioxidant potential of Indian stingless bee propolis was demonstrated to substantiate its anticancer activity.
In-Vitro Cytotoxicity of Indian Bee Propolis on Cancer Cell Lines
International journal of pharma and bio sciences, 2014
Propolis is a natural resinous hive product collected by honeybees from exudates of plant sources. It has been used in folk medicine due to its various chemical compounds with biological properties since ancient times. The aim of this study was to compare the chemical constituents and to evaluate the in-vitro cytotoxicity and hemolytic activity of Indian propolis against human breast cancer (MCF 7), colon cancer (HCT 116), and prostate cancer (PC 3) cell lines by MTT assay. The propolis extracts were incubated with cancer cell lines for 24 hours, cytotoxicity was measured colorimetrically and the IC50 value was calculated. Propolis samples were analyzed by GC-MS and 44 compounds were identified. The results indicated that inspite of the differences in the chemical composition of propolis collected from different geographic locations all the samples exhibited significant cytotoxic activity and did not affect normal cells. It is concluded that the Indian propolis is a potential natura...
Antitumor Activity of Propolis on Differantiated Cancer Cell Lines
Medicine Science | International Medical Journal, 2012
Propolis is a natural bee product with several pharmacological activities. Nowadays, it is also investigated for its antitumor properties. There are controversies on the antitumor activity of propolis, not all tumor cells seem to respond to propolis treatment. The aim of our study is to evaluate the activity of propolis on differentiated thyroid cancer cell lines. Tyripan blue test and MTT assay were performed to evaluate the cell viability of B-CPAP cells after propolis treatment and compared with propolis free cells and normal thyroid cells. Evaluation with light microscopy revealed after treatment with propolis, cell count decreased and B-CPAP cells displayed morphologic changes with a smaller appearance. Tyripan blue exclusion test showed a decrease in cell viability in different concentrations of propolis at 48 and 72 h. Cell viability was measured by MTT after the treatment of B-CPAP cells with propolis at 72 h. The decrease in cell viability was statistically significant only at 0.0001 diluted propolis (p=0.048). The decrease in cell viability in normal thyroid cell line was not statistically significant. In conclusion, propolis has weak antitumor activity on differentiated thyroid cell line which may contribute to different degrees of sensitivity of propolis among cancer cells.
Emirates Journal of Food and Agriculture, 2014
Propolis is a resinous substance collected by stingless bee or honey bee from various plant sources. The substance is known to contain beneficial properties for human. The geographical origin of propolis determines its biological properties. In this study, propolis were collected from five regions of Indonesia with the objective of determining the yield, their total flavonoid content, their capacity to induce apoptosis, and their toxicity to the Michigan Cancer Foundation-7 (MCF-7) cell line. The inhibition of antioxidant 1,1-diphenyl-2-picrylhydrazyl (DPPH), the induction of apoptosis to Saccharomyces cerevisiae and the anticytotoxic ability were determined. Propolis from Pekanbaru region had higher yield than other regions with value of 19.97%; propolis from Kendal had higher quantity with value of 46.60%, total flavonoid content; propolis from Pandeglang was higher in DPPH oxidation capacity with value of 68.94 μg.ml-1 ; propolis from Kendal, expressed petite cell induction in S. cerevisiae cells with value of 81.44%, and the anticytotoxic to MCF-7 breast cancer cell line were best observed in propolis from Makassar region with a value of 47.71% life cells. All of the propolis extracted from the stingless bee hive Trigona spp from five regions in Indonesia contained flavonoids.
Evidence-Based Complementary and Alternative Medicine, 2012
Propolis is a resinous product collected by honey bees. It was also reported that propolis has a wide variety of biological actions, including antimicrobial activity and antioxidant, anti-inflammatory, and suppressive effects of dioxin toxicity activities. The aim of this study was to compare the in vitro cytotoxic activities of green propolis (G12) and red propolis (G13) in human leukemia cells. These cells were incubated with different concentrations of propolis and 48 hours after the IC 50 was calculated for each cell. The results showed that the red propolis has cytotoxic effect in vitro higher than green propolis. Red propolis was showed to be cytostatic in K562 cells and caused the same amount of apoptosis as its control Gleevec. In conclusion, these results showed that red propolis is more cytotoxic than the green propolis in a variety of human cell lines of leukemia. Red propolis may contain drugs capable of inhibiting cancer cell growth. Therefore, further isolation of respective chemical ingredients from the red propolis (G13) for identification of the activities is necessary.
A Pilot Study on the Immunomodulatory Effect of Bulgarian Propolis
Biotechnology & Biotechnological Equipment, 2010
Propolis is a product from the honey bee A. mellifera with various pharmacological properties. Its immunomodulatory activity is in the focus of the current research. Peripheral blood mononuclear cells (PBMC) from heparinized venous blood of healthy donors (n=6) were cultured for 24 h in the presence of propolis from the Eastern Rodopi Mountain (ethanol extractions with concentration 0, 01; 1; 2,5; 5 and 10 mg/L) or CAPE in concentration 2, 4, 8 and 16 mg/L. PBMC cultured in serum free RPMI only were used as controls. The percentage
Cytotoxic Effect of Cuban Propolis Extracts Against Tumor Cells Lines
Propolis is a resin formed by a complex mixture of chemical substances that bees collect from plants. Since ancient times, propolis has been used in folk medicine due to its biological properties that include antimicrobial, antioxidant, anti-inflammatory, and antitumoral activities. The aim of this study was to compare the in-vitro cytotoxic activities of 16 Cuban propolis extracts of different chemical types: brown (1, 4, 5, 16, and 17 BCP), red (9, 29, 35, 37, 45 and 72 RCP) and yellow (18, 39, 41, 50 and 60 YCP) against murine breast carcinoma F3II, human breast adenocarcinoma MDAMB- 231, human lung adenocarcinoma A549, and human fibroblast MRC-5. These cells were incubated with different concentrations of propolis and 72 hours after, the IC50 was calculated for each cell. The results showed the antiproliferative effect of Cuban propolis against all cell lines tested. RCP was the most interesting group because they did not affect normal cells. RCP-45 extract showed the highest se...
Biological Effects of Propolis on Cancer
Turkish Journal of Agriculture - Food Science and Technology, 2020
Propolis is a special resin and wax material collected from the leaves and shells of trees, buds and shoots of plants by honey bees (Apis mellifera L.). In recent years, many researchers have studied the chemical composition, biological activity and pharmacological properties of propolis. The colour, odour and pharmacological properties of the propolis composition also vary as the composition changes depending on the plant, region, season and bee colony. Flavonoids, aromatic acids, phenolic acids and esters are the main compounds responsible for the biological activity of propolis. A number of studies have been conducted on the use of propolis or its active ingredients in the treatment of cancer. It has been observed that the use of propolis did not cause side effects according to in-vitro and in-vivo studies. Propolis should be extracted with different compounds for use in biological assays. The most commonly used compounds for extraction are ethanol, methanol, oil and water. A num...