Effect of Metosartan on Male Reproductive Potential in Normal and Hypertension Induced Rats (original) (raw)
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Effects of Metosartan on Testis Tissue Chromatin and Enzyme Kinetics of Testicular RNaseA
2019
Metosartan is the drug normally used as antihypertensive agent that act on heart and reduces hypertension in humans. It's components telmisartan is a angiotensin type I receptor blocker known to induce apoptosis in urinary bladder and metoprolyl is the beta receptor blocker. RNase A is the most experimental protein in the 20th century. Disulphide bonds are necessary for enzymatic action of many proteins and it is also required for this protein. RNaseA kinetic studies is performed with the drug metosartan using RNA as the substrate Metosartan is an combination of metoprolol and telmisartan and found to contain inhibitory property on RNaseA. Protein degradation and thiol titration assay has found to be that the drug has reducing property on RNaseA. The study concludes that metosartin inhibits the activity of RNAse A allosterically by reducing the disulphide bonds present on it. The reduction of disulphides may dissociate the enzyme to its monomer which is not highly active hence reducing the catalytic activity of RNase A. HPLC studies confirm the RNaseA presence in testes and drug also induce toroid packing of DNA in immature sperms.
Review on Effects of Metosartan on Testes Tissue
Metosartan induces so many deleterious effects in male Wistar rats treated with drug and RNase. It causes pre maturation of sperms and reduces the sperm count as well as reduces viable sperm count. It induces the x-chromosome desynapsis leading to testicular carcinoma. The effects are so profound in case of drug than compared to the enzyme RNase. Metosartan induces apoptosis in testicular tissue mainly through intrinsic pathway which is mainly due to genotoxic agents. The drug mainly inhibits RNase A in rat testes and has positive effect on Mitosis.
Toxicants and human sperm chromatin integrity
Molecular Human Reproduction, 2010
The integrity of the paternal genome is essential as the spermatozoon can bring genetic damage into the oocyte at fertilization and contribute to the development of abnormal pregnancy outcome. During the past two decades, many assays have been developed to measure sperm DNA strand breaks, chromatin structure and compaction and assess the proteins associated with the DNA, as well as epigenetic modifications. Using these assays, it has been shown that exposure to physical agents or chemicals, including therapeutic drugs and environmental toxicants, can affect the integrity of sperm chromatin, inducing structural, genetic and/or epigenetic abnormalities. The mechanisms by which such damage is triggered are still largely unresolved and the susceptibility of each individual will depend on their genetic background, lifestyle and exposure to various insults. Depending on the nature of the chemicals, they may directly target the DNA, induce an oxidative stress, or modify the epigenetic elements. The significance of measuring the sperm chromatin integrity comes from the fact that this end-point correlates well with the low IVF and ICSI outcomes, and idiopathic infertility. Nevertheless, it is hard to establish a direct link between the paternal sperm chromatin integrity and the health of the future generations. Thus, it seems essential to undertake studies that will resolve the impact of chemical and environmental factors on chromatin structure and epigenetic components of human spermatozoa and to elucidate what sperm nuclear end-points are predictors of the quality of progeny outcome.
Chromosomes form the genetic material of germ cells which constitutes the major part of both female and male reproductive system.. Damage to it was caused by the several agents like the drug metosartan which causes severe damage to chromatin leading to male infertility as sperm chromatin is also part of it, so it is necessary to design the methods that prevent the damage immediately. Chromatin imaging by agarose embedding method of DNA localisation from testis formulated in this article through simple fluorescent microscope and imaging techniques Was proved that the drug was toxic to the male reproductive system.
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Male infertility has been increasing over the last decades being nowadays a pressing health problem. Diabetes mellitus (DM) can contribute directly or indirectly to male infertility due to an abnormal spermatogenesis, which results in a decreased sperm quality. Type 2 diabetes mellitus (T2DM) is responsible for the vast majority of DM cases, being frequently treated with oral antidiabetic drugs. Metformin is the most cost-effective therapy for the treatment of T2DM. This biguanide is an oral insulin-sensitizing agent capable of increasing insulin sensitivity and decreasing plasma fasting insulin levels. The main metabolic action of this drug occurs in the liver. However, it has been shown that metformin acts on a variety of organs including the male reproductive system. With the rising numbers of diabetic individuals among younger populations, there is an increase in the consumption of metformin in individuals of this age group. As a result, it is important to discuss the role of me...