Uncovering Northeast Portugal grapevine's varietal legacy (original) (raw)
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Grapevine Diversity and Genetic Relationships in Northeast Portugal Old Vineyards
Plants, 2021
More than 100 grapevine varieties are registered as suitable for wine production in “Douro” and “Trás-os-Montes” Protected Designations of Origin regions; however, only a few are actually used for winemaking. The identification of varieties cultivated in past times can be an important step to take advantage of all the potential of these regions grape biodiversity. The conservation of the vanishing genetic resources boosts greater product diversification, and it can be considered strategic in the valorisation of these wine regions. Hence, one goal of the present study was to prospect and characterise, through molecular markers, 310 plants of 11 old vineyards that constitute a broad representation of the grape genetic patrimony of “Douro” and “Trás-os-Montes” wine regions; 280 samples, grouped into 52 distinct known varieties, were identified through comparison of their genetic profiles generated via 6 nuclear SSR and 43 informative SNP loci amplification; the remaining 30 samples, ac...
Ciência e Técnica Vitivinícola, 2015
Nineteen SSR were used to genotype twenty eight accessions from Portugal, France, Greece, and Spain, preserved at the Portuguese National Ampelographic Collection (international code-PRT 051). Some of these accessions have never been genotyped and are minor, underused cultivars. Eight cases of synonymies and one case of homonymy were detected. The identification of synonyms in underused cultivars increases de possibility of obtaining adequate plant material for certification purposes and commercialization. The new unique genotypes identified in this work will be proposed for inclusion in the list of authorized cultivars for wine production in Portugal. No certified plant materials exist for these cultivars. RESUMO Dezanove SSR foram utilizados para genotipagem de vinte e oito acessos de Portugal, França, Grécia e Espanha, preservados na Coleção Ampelográfica Nacional (código internacional-PRT 051). Algumas destas cultivares nunca foram genotipadas e são cultivares minoritárias, subutilizadas. Oito casos de sinonímia e um caso de homonímia foram identificados. A identificação de sinonímias em cultivares subutilizadas aumenta a possibilidade de encontrar material vegetal adequado para fins de certificação e comercialização. Os novos genótipos únicos identificados neste trabalho serão propostos para inclusão na lista de cultivares autorizadas para produção de vinho em Portugal. Estes novos genótipos não possuem material vegetal certificado para propagação.
2015
A broad prospection of grapevine plants was carried out in northwest Spain and north of Portugal during the period from 2002 to 2009. It included zones located in Galicia as well as in the provinces of León, Zamora and Salamanca in Spain and the region delimited by the right side of the Douro river and the spanish border in Portugal. A total of 669 accessions were sampled, studied and identified, both by using the six microsatellite loci included in the OIV descriptors list, plus ampelographic characters. As a result of the study a total of 53 different grapevine varieties were identified. Most of them correspond to minor varieties that are present in the zone. In many cases the same variety was detected in both countries, although denominated with different names that are synonymous. The molecular markers that were analyzed are those included in the OIV 801 to 806 descriptors, and correspond to the following loci: VVS2, VVMD5, VVMD7, VVMD27, ssrVrZAG62 and ssrVrZAG79. Allele sizes ...
Identification of minority grapevine cultivars from Vinhos Verdes Portuguese DOC Region
2015
The Portuguese Vinhos Verdes DOC Region retains a considerable biodiversity in grapevine varieties. Unfortunately, regional grapevine patrimony is in danger of disappearing. The present study was carried out in order to genotype, using 12 microsatellite loci, a group of 39 accessions of Vinhos Verdes DOC region, including underuse and minor varieties included in the wine list of varieties and also several unknown accessions with regional designations. The accessions analyzed were identified and grouped into 34 different genotypes, nine of them referred as new genotypes. Some new synonyms were detected, namely between Spanish and Portuguese cultivars. Misidentifications and wrong designations were also detected.
Synonymy in Grape (Vitis Vinifera L.) Cultivars of Northern Portugal and Northwestern Spain
Acta Horticulturae, 2011
Between 2002 to 2009, samples of grape plants were obtained from northwest Spain and northern Portugal. More than 650 accessions were sampled, studied and identified, using the six microsatellite loci included in the last edition of the International Organisation of Vine and Wine (OIV) descriptors list, plus ampelographic characters evaluated in old leaves, bunches and berries. A total of 26 grapevine cultivars, 12 white and 14 black were identified. Several synonyms and homonyms were detected. Some of the synonyms mentioned in the literature were discussed. The primary Spanish and Portuguese names are given to avoid misidentification and errors in the determination of specific genotypes of grape cultivars. White Cultivars 'Albariño', an important cultivar in Galicia, is the 'Alvarinho' from Portugal. This synonymy was previously cited (OIV, 1996). No other synonyms are known. 'Cagarrizo', found in several locations is the Portuguese cultivar 'Folgasão'. 'Caiño Blanco' or 'Caiño Branco' from Galicia is the 'Cainho de Moreira' from north Portugal, where it is also known as 'Alvarinhao', a name that leads to possible misidentifications with 'Alvarinho'. 'Godello' receives this name in Galicia; called 'Verdejo Blanco' or only 'Verdejo' in Arribes del Duero. In Portugal it is named 'Gouveio' or 'Verdelho Branco', a synonymy previously cited (
Editora Artemis eBooks, 2023
O conteúdo deste livro está licenciado sob uma Licença de Atribuição Creative Commons Atribuição-Não-Comercial NãoDerivativos 4.0 Internacional (CC BY-NC-ND 4.0). Direitos para esta edição cedidos à Editora Artemis pelos autores. Permitido o download da obra e o compartilhamento, desde que sejam atribuídos créditos aos autores, e sem a possibilidade de alterá-la de nenhuma forma ou utilizá-la para fins comerciais. A responsabilidade pelo conteúdo dos artigos e seus dados, em sua forma, correção e confiabilidade é exclusiva dos autores. A Editora Artemis, em seu compromisso de manter e aperfeiçoar a qualidade e confiabilidade dos trabalhos que publica, conduz a avaliação cega pelos pares de todos manuscritos publicados, com base em critérios de neutralidade e imparcialidade acadêmica.
Recovering ancient grapevine cultivars in the Spanish provinces of Alicante and Valencia
Acta Horticulturae, 2019
The provinces of Alicante and Valencia, located in the Mediterranean coast of Spain, are important viticulture areas since historic times. In the context of the research project CGL2015-70843-R, we initiated different approaches in order to recover ancient varieties threatened of disappearance and we developed in vitro protocols for virus sanitation and in vitro conservation of the recovered germplasm. Among the historic varieties, we localized several accessions of varieties that were commonly grown in the pre-phylloxera era in the provinces of Alicante and Valencia (i.e. 'Valencí blanc', 'Valencí negre', 'Planta Mula', 'Botó de Gall' or 'Raïm del Clotet'). Microsatellite profiles were obtained to confirm or identify the surveyed germplasms and genetic variability was observed. In addition, a survey was carried out covering the main area of 'Monastrell' cultivation in the Vinos de Alicante Protected Designation of Origin. This ancient variety, also known as 'Mourvèdre', is cultivated mainly in the southeast of Spain and it is highly adapted to the dry and warm climate of this area. Genotyping by Sequencing (GBS) was used to estimate the genetic diversity of this variety and high variability was found. This analysis will provide a high number of high quality SNPs well distributed across the genome suitable for genotyping of clones, which will allow the design of strategies optimizing their conservation and use.
Identity of three grapevine varieties from a rediscovered viticulture region in northwest Spain
Journal International des Sciences de la Vigne et du Vin
Aims: The old literature contains references to the varieties once cultivated in the Betanzos region (northwestern Spain) and three of them (Blanco Legítimo, Agudelo and Serradelo) were rediscovered during a survey work that began in 1987 and now form part of a collection held at the Misión Biológica de Galicia (MBG-CSIC). The aims of the present work were 1) to describe these three varieties, 2) to determine whether grapevines recently planted are indeed true representatives of these varieties and if so 3) to examine their agronomic and oenological potential. Methods and Results: Ampelographic descriptions were made following the OIV method (OIV, 2009) and the reconstruction of ‘mean leaves’ (Martínez and Grenan, 1999). Ten microsatellite loci were also characterised. Having confirmed the supposed identity of the vines in the vineyards, their agronomic and oenological potentials in the region were investigated (fertility, weight of fruit, composition of must and wine). The ampelogr...
Plants, 2020
A prospecting work at the Axarquia region (Malaga, Spain) was carried out in order to identify local red grapevine cultivars preserved in ancient vineyards. A total of 11 accessions were collected in seven different plots from four municipalities and analyzed using 25 microsatellite loci for cultivar identification. The accessions analyzed were identified as eight different genotypes, seven of them corresponding to known cultivars as ‘Cabernet Sauvignon’, ‘Jaen Tinto’, ‘Molinera’, ‘Monastrell’, ‘Muscat of Alexandria’, ‘Parrel’, and ‘Romé’. In addition, one of them is referred to as the new genotype for ‘Cabriel’ cultivar. Additionally, an ampelographic characterization was carried out with 30 International Organisation of Vine and Wine (OIV) descriptors for two consecutive years for the eight accessions identified as local cultivars. This allowed the identification of a somatic variant of the ‘Muscat of Alexandria’ cultivar that affects the color of the berry and another of ‘Romé’ r...
Genetic Resources and Crop Evolution, 2009
Portugal has a long tradition in viticulture and a great number of grapevine cultivars. To analyze the genetic relations among wild vines from Portuguese populations and old Portuguese grapevine cultivars we use morphological traits and chloroplastidial microsatellites from 53 accessions of four distinct populations of Vitis vinifera L. ssp. sylvestris (Gmelin) and 57 accessions of Vitis vinifera L. ssp. vinifera from the Portuguese National Ampelographic Collection. Principal coordinate analyses with the scores obtain from the descriptors of both the accessions of sylvestris and vinifera vines revealed two groups. One group is formed by the wild vine population of Alcácer do Sal and three vinifera accessions Rufete, Seara Nova and Trincadeira das Pratas and a second group includes all the other wild vines and grapevine cultivars. A total of four different chlorotypes (A, B, C and D) are present in the vinifera accessions and two in the sylvestris accessions (A, B). Chlorotype A is the most frequent in all the plants analyzed and correspond to 75.4% of the grapevine cultivars and 66% of the wild vines. The mixed distribution of chlorotypes in the Portuguese cultivars and the predominance of chlorotype A both in its wild populations and cultivars reinforced the hypothesis that West Europe was a domestication center for Vitis vinifera L. ssp. vinifera.