LC-MS/MS detection of pro-MPO in affinity-purified plasma separated by SDS/PAGE (original) (raw)

2018

Abstract

<p>Plasma was purified by affinity chromatography with an MPO-specific antibody and subjected to separation by SDS/PAGE. Bands with the molecular weight of pro-MPO (90 kDa) were subjected to in-gel tryptic digestion and analyzed by LC-MS/MS using SRM for a pro-MPO-specific peptide (1000.9->1033.5). <b>(A)</b> Extracted ion chromatogram for the SRM transition for the pro-MPO specific peptide. <b>(B)</b> CID-MS/MS spectrum confirming the sequence for the pro-MPO-specific peptide SSGcAYQDVGVTcPEQDK. Representative chromatograms and spectra are shown.</p

Irada Khalilova hasn't uploaded this paper.

Let Irada know you want this paper to be uploaded.

Ask for this paper to be uploaded.