Microscopical and Serological Studies with Ultrastructure Description of Sarcocystis Species in Sheep in Assiut (original) (raw)
Related papers
2014
Percoll gradient centrifugation can purify different parasitic stages from tissues or faeces of hosts. Sarcocystis tenella and Sarcocystis arieticanis develop microscopically visible cysts in sheep muscles. In this study, the distribution of microscopic cysts were determined in different muscles groups of sheep naturally infected with Sarcocystis spp. by using percoll gradient centrifugation. Sarcocystis spp. microscopic cysts were detected as 91% of sheep. S.tenella and mixed infections with S.tenella and S.arieticanis were observed in the tissue samples at a prevalence of 91% and 18.7%, respectively. Sarcocystis species cysts were most prevalent in the tongue muscle tissue at a rate of 80%. The cysts were observed a rate of 73%, 69% and 61% in the masseter, intercostal muscles and diaphragm, respectively. The relationship between the cysts present and the different muscles groups was significantly different (p<0.001). The number of microscopic cysts ranged from 4-476 (mean 235)...
Ultrastructure of the Cyst Wall of Sarcocystis Sp. In Roe Deer
2000
Samples of heart, tongue, oesophagus and diaphragm muscle from twenty-two nat- urally infected roe deer (Capreolus capreolus) harvested in central Italy were examined for sar- cos1)Oridiasis. The structure of Sarcocystisspp. muscle cysts was examined by light and electron microscopy. Only one type of thin-walled cyst was distinguished by light microscopy. Electron microscopy showed cysts having a thin highly folded primary
IJVST-2020, Vol. 12, No.2, 2020
This study aimed to determine and identify Sarcocystis spp. infection in sheep of Mashhad city, Iran. From October 2018 to May 2019, the entire esophagus and diaphragm from 100 slaughtered sheep were collected from the Mashhad abattoir. Initially, samples were inspected by the naked eye for the presence of macrocysts. Also, all samples were examined for Sarcocystis spp. by tissue impression smear, histopathology, and PCR tests. Additionally, eight samples were inspected by transmission electron microscopy (TEM) and gene sequencing to confirm species identification. The infection rate of sarcocystosis by impression smear, histopathology, and PCR methods were 69%, 96%, and 100%, respectively. Histopathological examination revealed the existence of S. gigantea macrocyst with PAS-positive secondary cyst wall in 26% of sheep. Also based on cyst wall morphology, two types of microcysts including S. tenella with striated thick cyst wall and S. arieticanis with smooth thin cyst wall were identified in 47% and 11% of sheep, respectively. By TEM, the cyst wall of S. gigantea had cauliflower-like, S. tenella had finger-like and S. arieticanis had hair-like villar protrusions. Comparative analyses of the sequencing of the 18S rRNA gene revealed S. gigantea, S. tenella, and S. arieticanis in PCR samples. The results showed that the infection rate of Sarcocystis spp. was very high by the PCR method. Also, the existence of S. gigantea, S. tenella, and S. arietcanis species was confirmed by histopathology, TEM, and DNA sequencing methods in sheep of this area.
Veterinary Parasitology, 2011
Cysts of Sarcocystis capracanis obtained from infected goats were examined to clarify the effect of the parasite on the host. Muscle tissues from fresh oesophagus, tongue, diaphragm and skeletal muscles of 680 goats were slaughtered in the main abattoir of Cairo, Egypt and they were examined microscopically for Sarcocystis infection for the first time in Egypt. 540 out of 680 (79.4%) of examined goats were found to be infected with Sarcocystis sp. The infection was recorded firstly by light microscopy as spindle shaped cysts embedded in the muscle tissues. The validity of this species as S. capracanis was confirmed by means of ultrastructural characteristics of the primary cyst wall which revealed the presence of thick-radially striated wall with finger like projections, underlined by a thick layer of ground substance enclosing the developing metrocytes and merozoites that usually contain nearly all the structures of the apical complex and fill the interior cavity of the cyst. The cyst cavity is divided by many septa extending from the ground substance and producing large number of chambers. An experimental infection using the highly infected muscles was carried out to determine the final host, which is dog. Smears of intestinal epithelium were taken to examine the endogenous stages (gamogony and sporogony) by means of light microscopy. These stages were mainly observed as to infect the lamina propria of the posterior third of the small intestine. Gamogony and zygote formation (fertilization) occurred 2-8 days post infection, while sporulation took place within the final host 13-15 days and sporocysts were passed within faeces of the infected puppies at that time. The prepatent period of S. capracanis was 12-15 days, while the patent period was extended to 37 days. In goats, infection with S. capracanis led to the loss of weight, anaemia, abortion and even death in cases of heavy infection. While bleeding, watery faeces filled with mucous on 5th and 8th day p.i. as well as intestinal lesions are the pathogenic effects occurred in puppies after experimental infection.
International journal of molecular and cellular medicine, 2014
Sarcocystis is an obligatory intracellular protozoan parasite which can infect humans and animals. Sheep are intermediate hosts of four Sarcocystis species: Sarcocystis tenella, Sarcocystis gigantea, Sarcocystis arieticanis, and Sarcocystis medusiformis The purpose of this study was to perform a molecular identification of the macroscopic and microscopic cysts of Sarcocystis in sheep. In this investigation, the macroscopic and microscopic cysts of Sarcocystis were assessed in slaughtered sheep. The digestion method was used for bradyzoites observation in heart, liver, diaphragm and muscle samples. PCR analysis was conducted on macroscopic and microscopic cysts and also all other samples. Sequencing was performed for ten PCR products. Genotypes were identified by BLAST search and homology analysis. Macrocysts were seen in two muscle tissues. Digestion method and PCR analysis revealed positive results in all samples taken from heart, liver, diaphragm, and muscle. Genotyping of ten tis...
Parasitology, 2015
There is considerable confusion concerning Sarcocystis species in camels. Five species: Sarcocystis cameli, Sarcocystis ippeni, Sarcocystis camelicanis, Sarcocystis camelocanis and Sarcocystis miescheri were named with inadequate descriptions and no type specimens. Here, we review literature on sarcocystosis in camels worldwide and redescribe structure of S. cameli and S. ippeni sarcocysts by light-and transmission electron microscopy (LM and TEM). Eight sarcocysts from the oesophagi of two camels (Camelus dromedarius) from Egypt were studied. By LM, all sarcocysts were thinwalled with barely visible projections on the cyst walls. By TEM, two structurally distinct sarcocysts were recognized by unique villar protrusions (vp) not found in sarcocysts from any other host. Sarcocysts of S. cameli had vp of type 9j. The sarcocyst wall had upright slender vp, up to 3·0 µM long and 0·5 µM wide; the total thickness of the sarcocyst wall with ground substance (gs) layer was 3·5 µM. On each vp, there were rows of knob-like protrusions that appeared to be interconnected. The vp had microtubules that originated at midpoint of the gs and continued up to the tip; microtubules were smooth, without any granules or dense areas. Bradyzoites were approximately 14-15 × 3-4 µM in size with typical organelles. Sarcocystis ippeni sarcocysts had type 32 sarcocyst wall characterized by conical vp with an electron dense knob. The total thickness of the sarcocyst wall (from the base of gs to vp tip) was 2·3-3·0 µM. The vp were up to 1·2 µM wide at the base and 0·25 µM at the tip. Microtubules in vp originated at midpoint of gs and continued up to tip; microtubules were criss-crossed, smooth and without granules or dense areas. Bradyzoites were 12·0-13·5 × 2·0-3·0 µM in size. Sarcocystis camelicanis, S. camelocanis and S. miescheri are considered invalid.
Parasitology Research, 2009
In the present study, the heteroxeneous life cycle of Sarcocystis sp. infecting camels were studied. A total of 180 slaughtered camels collected from different localities in Egypt were investigated for sarcocysts. Only 116 animals were found to be infected (the infection rate was 64%). Muscle samples of esophagus, diaphragm, tongue, skeletal, and heart muscles were examined. Exclusively, microscopic sarcocysts were detected in all examined organs. The infection rates of the esophagus, diaphragm, tongue, skeletal, and heart muscles were 60%, 50%, 40%, 40%, and 10%, respectively. By means of transmission electron microscopy, details of the ultrastructure of the sarcocysts were studied. The specific architecture and ornaments of the cyst wall, its protrusions, and the cyst interior were recorded. Unique features of protrusions of the primary cyst wall, the knob-like structures, arise around each protrusion. Experimental infection of carnivores by feeding heavily infected camel muscles revealed that the dog, Canis familiaris, is the only final host of the present Sarcocystis species. Gamogony, sporogonic stages, and characteristics of sporulated oocysts were also investigated.
Light and ultrastructure of Sarcocystis spp. of camels and associated pathological changes
Samples of oesophagus, diaphragm, heart, lungs and kidneys were collected from 93 camels slaughtered at El-Basateen and El-Warak abattoirs, Egypt (from June to December 2012). Oesophagus, diaphragmatic and heart muscles were examined macroscopically by naked eyes and microscopically by light and transmission electron microscope (TEM). No macroscopic Sarcocystis cysts were detected by naked eyes. Microscopic cysts were detected by compression technique with light microscope in 64/93 (68.8%). The positive cases in oesophagus, diaphragm and heart were 54/80 (67.5%), 34/56 (60.7%) and 0/11 (0%) respectively. Light microscopic examination of oesophagus and diaphragm revealed dark cylindrical and spindle shaped cysts, but some spindle shaped cysts in diaphragm appeared light in color. In cylindrical cysts some parts appeared spiral in shape. Also, some small sized cysts appeared spiral in shape. Some cysts in diaphragm appeared elongated with one rounded end while the other was more or le...
Asian Journal of Agriculture and Biology, 2020
This study focused on the detection of Sarcocystis spp. in two species of rats, Rattus norvegicus and R. tanezumi collected from agricultural area in Dasmarinas, Cavite. This aimed to corroborate the presence of Sarcocystis spp. in different species of rats found in the agricultural area. Further the establishment of different Sarcocystis spp in Rattus spp. based on the parasite's morphologic characterization is also emphasized. Sixty-nine rats (36 R. norvegicus and 33 R. tanezumi) were collected through trapping. Individual rodent autopsy for host identification was performed by determining the morphological differences and external measurements prior to dissection. Tissue samples were examined for the presence of white rice-grain sized nodules. Morphological characteristics of the cysts, particularly the size, shape, and presence of protrusions were noted. Statistical analysis using ANOVA for the significant difference on the number of infected rats per species and across muscle type was done at p≤0.05. Infection in R. tanezumi was higher (48.48%) as compared to R. norvegicus (41.67%), the difference however was not significant. Sarcocysts burden was higher in the diaphragm in both rat species. Generally, higher parasite load was observed in R. norvegicus. In both rat species, tissue cysts ranged from spindle-shaped/fusiform to globular and oval-shaped. The sarcocysts in the diaphragm were more varied. Consistently observed in the tongue were globular-shaped sarcocysts while either fusiform-and globular-shaped sarcocysts in the diaphragm. These differences suggest infection with more than one species of Sarcocystis. Hence, the presence of Sarcocystis spp. confirmed the infectivity of the parasite to Rattus spp. Moreover, the different morphologic characteristics observed on Sarcocystis prove the possibility of different species of the parasite harboring the Rattus spp.