Chemical composition and antioxidant activity of essential oil from leaves and rhizomes of Curcuma angustifolia Roxb (original) (raw)
Related papers
Evaluation of Physicochemical and Antioxidant Potential of Fixed Oil from Curcuma Longa Linn
Trends in natural products research , 2021
Curcuma longa is used in traditional medicine in Nigeria for the treatment of diseases with etiologies linked to free radicals. This study was designed to investigate the physicochemical, phytochemical and antioxidant properties of the oil extracted from Curcuma longa rhizome. The plant was collected from its natural habitat and then identified. The sliced air-dried rhizome was powdered using grinding mill. The physicochemical parameters of the powdered rhizome were determined using standard procedures. The oil was extracted from the powdered rhizome using nhexane. The solubility of the oil and the specific gravity were determined using standard methods. The oil was screened qualitatively for phytochemicals constituents and then analyzed for its chemical constituents using GC-MS. The acid value (AV), saponification value (SV), ester value (EV) and iodine value (IV) were determined. The acute toxicity was determined in mice using the Organization for Economic Cooperation and Development (OECD) method. The free radical scavenging activity was assayed using DPPH assay. The result of the physicochemical studies revealed moisture content (9.5 %) total ash (8.0 %) acid insoluble ash (3.5 %,), alcohol extractive value (4.8 %) and water extractive value (5.2 %). The percentage yield of the oil was 3.53 %. The oil was soluble in most of the solvents tested. The specific gravity of the oil was 0.98. The qualitative phytochemical screening of the oil revealed the presence of steroids and terpenoids. The GC-MS analysis of the oil showed the presence of tumerone, atlantone, caryophyllene, α-terpeniol, eugenol, curlone, octanoic acid-3-phenylpropyl ester and bisabolene. The AV, SV, EV and IV were 308.55
Natural Product Research, 2019
Hydro-distilled essential oils, from fresh rhizomes and leaves of Curcuma mutabilis Skorni ck., M.Sabu & Prasanthk., characterized by GC-MS revealed the presence of thirty three and twenty three compounds therein respectively. Whilst estrone methyl ether (3-Methoxyestra-1,3,5(10)-trien-17-one) was the major component in rhizome oil (47.35%), sesquiterpene hydrocarbons predominated as the major group (63.92%) in leaf oil with a higher preponderance of b-caryophyllene (25.48%), b-farnesene (19.47%) and a-humulene (11.01%). Weak antioxidant activities observed in these oils determined by DPPH and ABTS methods were apparently influenced both by the oil composition and the assay conditions. Rhizome oil showed higher antiproliferative activity than leaf oil against leukemic K562 (IC 50-6.8mg/mL) and colorectal HCT116 (IC 50-8.5mg/mL) cancer cell lines. This first report reveals composition and biological activities of essential oils from C. mutabilis.
Plants
Species belonging to the Zingiberaceae family are of high nutritional, industrial, and medicinal values. In this study, we investigated the effect of processing steps (fresh vs. dried milled rhizomes) and extraction methodologies (hydrodistillation vs. hexane extraction) of curcuma essential oil on its chemical content (using GC-MS analysis), its antioxidant behavior (using in vitro assays such as DPPH, ABTS, CUPRAC, FRAP, phosphomolybdenum, and metal chelation), and its enzyme inhibitory activities (on tyrosinase, acetylcholinesterase, butylcholinesterase, α-amylase, and α-glucosidase) supported by multivariate analysis, in silico studies, and molecular dynamics. The GC-MS investigations revealed a high degree of similarity in the chemical profile of fresh hydrodistilled and hexane-extracted essential oils with tumerone and curlone being the major metabolites. The extraction techniques affected the concentrations of other minor constituents such as terpinolene, caryophylla-4(12), 8...
In vitro antioxidant potential of the essential oil and leaf extracts of Curcuma zedoaria Rosc
Journal of Applied Pharmaceutical Science
In this study, we examined the chemical composition of the essential oil and tested the antioxidant potential of the oil and leaf extracts of Curcuma zedoaria Rosc. The chemical compositions of the oil were analysed by GCMS. Twenty-four compounds representing 92.4% of the total oil was identified. The antioxidative potential was evaluated using two separate methods, inhibition of free radical 1, 1-diphenyl-2-picrylhydrazyl (DPPH) and superoxide radicals scavenging activities assay. In the first case, the IC50 value of the oil was 14.8 ± 2.2. Among the extracts, the strongest activity was exhibited by the ethyl acetate extract (IC50 = 17.56 ± 1.6 μg/ml). In the superoxide radicals scavenging activities assay, ethyl acetate extract was superior to all other extracts (IC50 = 23.47 ± 1.2 μg/ml). Furthermore, the amount of total phenolic compounds was also determined as gallic acid equivalent. Thus, the natural products produced from C. zedoaria may be used in food and pharmaceutical ind...
Comprehensive Assessment of Antioxidant Activity of Essential Oils
Journal of Food Science, 2012
Essential oils have been studied for their unique ability to act as antioxidants. Antioxidant activities of 423 essential oils of 48 different botanical families were evaluated for their antioxidant activities as free radical scavenging agents using the 1,1-diphenyl-2-picrylhydrazyl method. Seventy-three oils showed 50% or more inhibition at a concentration of 1.25 mg/mL. The 73 most active oil samples were further evaluated for their scavenging activities using series of dilutions to estimate their EC 50 . The EC 50 of the 73 most active oils ranged from 4 to 2000 μg/mL. Oils having an EC 50 of less than 300 μg/mL (20 selected samples) were subjected to β-carotene bleaching antioxidant activity test and more detailed analysis including thin layer chromatography, gas chromatography/mass spectrometry, high performance liquid chromatography and bioautography. Essential oils of the botanical families Lamiaceae and Myrtaceae were the most effective antioxidants. Thymol and carvacrol were the major constituents in most of the essential oils of the family Lamiaceae and eugenol was the major terpene in all of the essential oils of the family Myrtaceae.
Antioxidant Capacity of Selected Plant Extracts and Their Essential Oils
Antioxidants, 2013
The main objective of this study was the screening of some selected aromatic plants very popular in Greece, with respect to their total phenolic content, antioxidant capacity, reducing activity, and oxidative stability. All plants were extracted with the conventional method, reflux with methanol. The essential oils of the plants were also analyzed for their antioxidant properties. The total phenolic content was determined by the Folin-Ciocalteu method using gallic acid as the standard, while the phenolic substances were identified and quantified by High Performance Liquid Chromatography (HPLC) coupled with a multi-wavelength ultraviolet-visible (UV-vis) detector. The antioxidant capacity of the plant extracts was measured by their ability to scavenge free radicals such as (a) DPPH (2,2-diphenyl-1-picrylhydrazyl) and, (b) ABTS (2,2′-azinobis-(3-ethylbenzothiaziline-6sulfonate). The Folin-Ciocalteu method proved the existence of antioxidants in the aromatic plant extracts. Taking into account the results of the DPPH and ABTS methods, the free radical scavenging capacity was confirmed. Eventually, all plants exhibited low but noticeable protection levels against lipid oxidation, as determined by the Rancimat test.
Journal of Medicinal Plant Research, 2011
Essential oil extracted from the aerial parts of plant by hydrodistillation from Rosmarinus officinalis was analyzed by gas chromatography/mass spectrometry and the components identified were 15. The major constituents of the essential oil were 1, 8-cineole (35.32%), trans-caryophyllene (14.47%), borneol (9.37%), camphor (8.97%), α-pinene (7.9%) and α-thujone (6.42%). The R. officinalis essential oil was screened for theirs in vitro antioxidant activities using three different and complementary assays: 1, 1diphenyl-2-picrylhydrazyl (DPPH), β-carotene bleaching test and reducing power. The results of the DPPH assay showed an IC 50 inhibitory concentration of 110.20 μg/ml. In the β-carotene bleaching test, the IC 50 valued was 20.00 μg/ml. At higher concentration (70 μg/ml), this essential oil exhibited a lower reducing power with an absorbance value of 0.72 ± 0.02. The corresponding value EC 50 was evaluated as 38.68 μg/ml. Comparison of the antioxidant properties of the investigated essential oil with those expressed by butylated hydroxytoluene (BHT), used as a positive control, showed that this oil exhibited a slightly weak antioxidant potential than BHT and therefore acts as natural antioxidant agent.
Food and Chemical Toxicology, 2010
The phytoconstituents of essential oil and ethanol oleoresin of fresh and dry rhizomes of turmeric (Curcuma longa Linn.) were analyzed by GC-MS. The major constituents were aromatic-turmerone (24.4%), alphaturmerone (20.5%) and beta-turmerone (11.1%) in fresh rhizome and aromatic-turmerone (21.4%), alpha-santalene (7.2%) and aromatic-curcumene (6.6%) in dry rhizome oil. Whereas, in oleoresins, the major components were alpha-turmerone (53.4%), beta-turmerone (18.1%) and aromatic-turmerone (6.2%) in fresh and aromatic-turmerone (9.6%), alpha-santalene (7.8%) and alpha-turmerone (6.5%) in dry rhizome. Results showed that alpha-turmerone, a major component in fresh rhizomes is only minor one in dry rhizomes. Also, the content of beta-turmerone in dry rhizomes is less than a half amount found in fresh rhizomes. The antioxidant properties have been assessed by various lipid peroxidation assays as well as DPPH radical scavenging and metal chelating methods. The essential oil and ethanol oleoresin of fresh rhizomes have higher antioxidant properties as compared dry ones. j o u r n a l h o m e p a g e : w w w . e l s e v i e r . c o m / l o c a t e / f o o d c h e m t o x
Journal of Medicinal Plants Research, 2011
Essential oil extracted from the aerial parts of plant by hydrodistillation from Rosmarinus officinalis was analyzed by gas chromatography/mass spectrometry and the components identified were 15. The major constituents of the essential oil were 1, 8-cineole (35.32%), trans-caryophyllene (14.47%), borneol (9.37%), camphor (8.97%), α-pinene (7.9%) and α-thujone (6.42%). The R. officinalis essential oil was screened for theirs in vitro antioxidant activities using three different and complementary assays: 1, 1diphenyl-2-picrylhydrazyl (DPPH), β-carotene bleaching test and reducing power. The results of the DPPH assay showed an IC 50 inhibitory concentration of 110.20 μg/ml. In the β-carotene bleaching test, the IC 50 valued was 20.00 μg/ml. At higher concentration (70 μg/ml), this essential oil exhibited a lower reducing power with an absorbance value of 0.72 ± 0.02. The corresponding value EC 50 was evaluated as 38.68 μg/ml. Comparison of the antioxidant properties of the investigated essential oil with those expressed by butylated hydroxytoluene (BHT), used as a positive control, showed that this oil exhibited a slightly weak antioxidant potential than BHT and therefore acts as natural antioxidant agent.