Isolation of a Pancreas-Specific Gene Located on Human Chromosome 14q31: Expression Analysis in Human Pancreatic Ductal Carcinomas (original) (raw)

We have isolated a novel cDNA (SEL1L) that shows cinomas (2) to cytogenetically evident gene duplication in sequence similarities to SEL-1, a gene identified as an endocrine pancreatic neoplasms (13). Two other chromoextragenic suppressor of the lin-12 hypomorphic mutant somal loci displaying homozygous deletions have been from Caenorhabditis elegans (7, 8). SEL1L exhibits a tisdescribed: the 13q12.3 locus, in the vicinity of the heredisue-specific pattern of expression: a single poly(A) / RNA tary breast cancer susceptibility gene BCRA2 (17), and species of 7.5 kb is abundantly expressed only in the at 18q21.1 (11). Allotype data suggest the existence of pancreas of healthy individuals, whereas low to undeadditional tumor suppressor genes at other chromosomal tectable levels are observed in other adult and in some loci (12). Enhanced expression of the erbB-3 gene was fetal tissues. Somatic hybrid panel and fluorescence in reported in human pancreatic cancer and was associated situ hybridization positioned this gene in the q31 band with advanced tumor stage and shorter survival after of human chromosome 14. The tissue-specific expression surgery (6). of this gene induced us to study its role in human pancre-Recently, we have isolated a phage clone containing atic carcinomas. Our analysis revealed that 17% of adea cDNA (named Ibd) that is expressed at high levels nocarcinomas of the pancreas did not express SEL1L to only in adult pancreas of healthy individuals. Northern a detectable level; however, no gross genomic alterations blots containing poly(A) / RNA (Clontech, Palo Alto, were apparent in the few hundred kilobases of the rele-CA) from several human fetal and adult tissues were vant region. ᭧ 1997 Academic Press used to assess the expression of the novel cDNA. A

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