Ultra-structural Study by Transmission Electron Microscopy: Effect of Omega-3 on Ovary Cell Organelles after Experimental Induced Poly Cystic Ovary Syndrome (original) (raw)
Related papers
2013
PCOS produces symptoms in approximately 5% to 10% of women of reproductive age (12–45 years old). It is thought to be one of the leading causes of female subfertility. To confirm the role of nutrition with omega-3 polyunsaturated fatty acid on controlled of experimental PCO induced by estradiol-valerat (PPA) in rats, this study was done. Wistar female rat (n=50) were allocated into control (n=10) and test groups (n=40), test group subdivided into groups of 4, G1, received omega-3 (60 mg/rat/orally/daily),G2 induced PCO by single injection of estradiolvalerate (4mg/rat/IM), G3 and G4 groups were induced PCO by single injection of estradiol-valerate (4mg/rat/IM),G3 & G4 groups was received omega-3 (60 mg/rat) ,and G4, received lower carbohydrate feeding ,for 60 consequence day. In sixty day 5cc blood samples and ovarian tissues of Rats in whole groups were removed and prepared to biochemical pathological analysis. hemorrhage, hyperemia and fibrosis were seen in pco groups, these side ...
Journal of Translational Medicine
BackgroundOmega-3 fatty acids (Ω-3 PUFAs) may help to improve health status in polycystic ovarian syndrome (PCOS) by reducing numerous metabolic disorders (insulin sensitivity, hyperinsulinemia, lipid profile, obesity and inflammation). To evaluate the current objective, 16 weeks (6 weeks of adjustment period followed by 10 weeks of collection period) research trial was planned to check the impact of different sources of Ω-3 PUFAs (synthetic Ω-3, flaxseed and fish oil) on nutrient digestibility, weight gain, productive (lipid profile, glucose and insulin), reproductive profile (progesterone, follicle stimulating hormone (FSH), estrogen, luteinizing hormone (LH) and prolactin) and histological study of ovarian tissues in Wistar female rats.MethodsForty-five rats of 130 ± 10 g weight were divided into 5 groups, each having 9 rats: NC (negative control without PCOS), PC (positive control with PCOS), SO (synthetic omega-3 containing ALA, EPA and DHA), FO (flaxseed oil) and F (fish oil) ...
Short chain fatty acid, acetate restores ovarian function in experimentally induced PCOS rat model
PLOS ONE
Background Polycystic ovarian syndrome (PCOS) is pathogenically characterized with hyperandrogenism and metabolic alterations, which often result in ovarian changes and infertility in women of reproductive age. Epigenetic changes have been linked to the development of PCOS. However, the involvement of epigenetic regulator, histone deacetylase (HDAC) in PCOS-driven ovarian dysfunction is not clear. Howbeit, the present study hypothesized that acetate, an HDAC inhibitor (HDACi) would protect against ovarian dysfunction in experimentally induced PCOS. Materials and methods Female Wistar rats weighing 120–150 g were randomly divided into four groups (n = 6). The groups received vehicle, sodium acetate (200 mg/kg), letrozole (1 mg/kg) and letrozole with acetate by oral gavage respectively. The administrations were done daily for 21 days. Results The rat model of PCOS had increased body weight and ovarian weight, 1-hr postload glucose and plasma insulin, testosterone and LH/FSH ratio as w...
Omega-3 Ameliorates Effects of Letrozole-Induced Polycystic Ovary Syndrome in Adult Female Rats
El-Minia Medical Bulletin, 2019
Background and aim: Polycystic ovary syndrome (PSOS) is a common disease of multifactorial etiology causing dangerous complications for women. The pathophysiology and intrinsic mechanisms underlying PCOS are complex and not fully determined. This study is designed to explore the mechanisms involved in PCOS in rats and to investigate the possible role of omega-3 in prevention of PCOS. Methods: 24 adult female albino rats were randomly classified into (1) Control groups (C): either non-treated (5 rats) or treated with the vehicle (3 rats), (2) Letrozole group (L): (8 rats), rats received letrozole orally (1mg/kg for 21 days) to induce PCOS and (3) Letrozole and omega-3 group (LO): (8 rats), in which PCOS was induced by letrozole and rats simultaneously received omega-3 (240 mg/kg orally) for 3 weeks. Histopathological examination of ovaries and biochemical analysis for serum was done. Results: In L group, ovaries showed features of polycystic ovary syndrome. The body weight, lipid peroxides, serum levels of insulin, testosterone, TNF-glucose, TGs, TC, and LDL were significantly increased associated with a significant decrease in serum HDL and FSH. In LO group the body weight, lipid peroxides and serum levels of insulin, testosterone, TNF-α, glucose, TGs, TC and LDL were significantly decreased associated with a significant increase in serum HDL. Microscopic examination of the ovaries revealed that LO group showed improvement of polycystic features. Conclusion: Administration of omega-3 led to improvement of PCOS as regard to the histopathological and biochemical changes most probably via its anti-oxidant and anti-inflammatory effects.
Evidence Based Women's Health Journal
Background: In Polycystic ovary syndrome (PCOS) life style and dietary changes are recommended as the first line of management; however, the optimal nutritional management is not certain yet. Polyunsaturated fatty acids (PUFAs), particularly long-chain (omega-3) PUFAs, is known to improve overall human health and its widely used in managing infertility, but its role in PCOS management is still uncertain. Objective: The purpose of this study was to assess if omega-3 fatty acids supplementation for Polycystic ovary syndrome patients will have a positive effect on body weight, Hirsutism score, Menstrual cyclicity, hormonal status and Doppler flow to the Uterine and Ovarian stromal arteries. Study Design: A randomized prospective trial. Patients and Methods: A total of 67 patients with Polycystic ovary syndrome were randomized into two groups. Group I patients who received omega-3 fatty acid 1 gram daily capsules and group II patients who received no treatment for 3 months. Changes in weight, BMI, menstrual cycle length, modified Ferriman Gallway score, serum FSH, LH ,free Testosterone levels and Doppler of ovarian stromal and uterine arteries between baseline and the 3 months study period were compared between the 2 groups. Results: After 3 months supplementation with omega-3 fatty acid capsules, there was a statistically significant decrease in menstrual cycle length in the study group in comparison to the control group. Also, there was a statistically significant decrease in the pulsatility index (PI) of the uterine artery Doppler in the study group, but there was no change in the weight, BMI, ovarian stromal blood flow. Also, measures of FSH, LH and free testosterone did not show statistically significant changes for either group. Conclusion: Omega-3 fatty acids supplementation is effective in improving menstrual cyclicity and uterine artery blood flow in patients with PCOS and may be an helpful option for some patients with PCOS especially those who have defective endometrial receptivity and subsequent infertility or repeated miscarriages because of increased uterine artery resistance.
Non-esterified fatty acids in the ovary: friends or foes?
Reproductive Biology and Endocrinology, 2020
A majority of common metabolic diseases can result in excessive lipolysis, leading to elevated levels of non-esterified fatty acids (NEFAs) in the body fluids. In females, increased NEFA levels in the follicular fluid markedly alter the functions of intrafollicular cells such as granulosa cells (GCs) and oocytes. Therefore, elevated levels of NEFAs have been suggested to be a significant player of subfertility in females of both human and economically important animal species such as cattle, buffalo, sheep, pig, chicken, and dog. However, the effects imposed by saturated and unsaturated fatty acids (SFAs and UFAs) on ovarian follicles are controversial. The present review emphasizes that SFAs induce apoptosis in granulosa and cumulus cells of ovarian follicles in different species. They further could adversely affect oocyte maturation and developmental competence. Many types of UFAs affect steroidogenesis and proliferation processes and could be detrimental for follicular cells, esp...
Therapeutic role of Omega-3 fatty acids in improving PCOS and PCOS related complications
DIET FACTOR (Journal of Nutritional & Food Sciences)
To our insight, despite a few preliminaries investigating the helpful impact of n-3 polyunsaturated unsaturated fats (PUFA) on polycystic ovary disorder (PCOS), not many studies are done to examine the impacts of n-3 PUFA utilization on PCOS inconveniences. In this manner, our point was to write a review to explore the potential impact of omega 3 and related components. An extensive, precise search was led in Embase, MEDLINE/PubMed, Google Scholar, and SCOPUS to recognize n-3 unsaturated fats are helpful for improving PCOS complications. In this review, the effect of omega-3 on PCOS, mechanism of omega 3, omega-3 and insulin obstruction, omega-3 and adipokines, omega-3 and lipid metabolism, omega-3 and endothelial capacity, and omega-3 and hormonal elements are mentioned. There are various systems by which n-3 PUFAs might apply their valuable consequences for PCOS, including weight loss, glycemic and hormonal hemostasis, mitigating, the guideline of adipokine creation, and improveme...
Morphological Changes in Ovaries in Rats with Experimental Polycystic Ovary
2020
Polycystic ovary syndrome is one of the important causes of infertility, metabolic syndrome. Complications of PCOS are a serious health hazard. The relevance of the study of morphological changes in the ovaries in rats is justified by the need to improve the treatment of PCOS. The experiment consisted in the fact that rats from the experimental group received testosterone for 7 days and were in constant artificial daylight for 60 days. As a result of histological examination of the ovaries in this group, multiple cysts, foci of fibrosis were found. Vaginal smears in rats from the experimental and control groups were collected and compared. The extracted ovaries of rats of the experimental and control groups were compared, the differences in colour according to Van Gieson X 100, haematoxylin-eosin X 40, haematoxylin-eosin X 100, B-G X 100 were analysed. Studies indicated oestrous cycle disturbance, hormonal changes in rats with experimental polycystic. The work can serve as material ...
Changes of The Uterine Tissue in Rats with Polycystic Ovary Syndrome Induced by Estradiol Valerate
Volume 11, Number 1, Apr-Jun 2017
Background: Polycystic ovary syndrome (PCOS) is one of the most common hormonal disorders that can lead to irregular menstrual cycles and hyperandrogenism. Reduced levels of progesterone and increased estrogen in these women can perpetually stimulate the endometrial tissue of the uterus. In this study, we assess the effect of PCOS induction by estradiol valerate (EV) in a rat model.
Zahedan Journal of Research in Medical Sciences, 2020
Background: One of the most common endocrine and metabolic disorders is Polycystic ovary syndrome (PCOS), which has been reported in about 10% of women during the reproductive age. Objectives: This study was designed to investigate the efficiency of α-Linolenic acid (ALA) on in vitro maturation (IVM) and the quality of mouse oocytes with PCOS. Methods: Female NMRI mice (30 - 35 day-old) were developed by the injection of 4 mg estradiol valerate dissolved in 0.2 mg sesame oil for 60 consecutive days. In the following, the PCOS ovaries were dissected and oocytes were cultured in the maturation medium supplemented with different dosages of α-linolenic acid (0, 50, 100 µM). The presence of the first polar body was considered the sign of the nuclear maturation of the oocyte. The expression of mitochondrial transcription factor A (TFAM) gene in mature oocytes was investigated by Quantitative Real-time PCR. Results: The in vitro maturation and TFAM gene expression rates of PCOS oocytes in ...