Isolation and Identification of Bacillus megaterium Bacteriophages via AFLP Technique (original) (raw)

Isolation of bacteriophage from soil sample

2016

Bacteriophage also known as phage, is a virus that attack to a specific bacteria and replicates inside bacteria cell to produce more phages. Bacteriophage can be found in sewage, soil, water and food. This virus kills bacteria by lysis the host cell. There are two cycles of phage which are Lytic Cycle and Lysogenic Cycle. The benefits of phage are they can be used to kill bacteria, as replacement of antibiotic, biological control agent, and phage application in food industry. Phage can also survive in any condition because they have ability to reproduce quickly, high level of specificity and long term survivability. Phages were isolated and partially purified using PEG/ NaCl. The titers of phages were determined by plaque assay methods. The soil sample has different population of bacteriophage. Phage is isolate from soil sample to determine what type of phage in soil. From this study, the characteristic of phage showed that the phage is a type of lytic phage because it has similarit...

A Laboratory Practicum on Screening for Lytic Bacteriophages from Soil Samples

Transactions on Science and Technology, 2018

Bacteriophages are viruses that explicitly infect and lyse bacteria. They are ubiquitous and play vital roles in various biotechnological applications such as phage-based bacterial detection and bacteriophage therapy. This paper describes a simple bacteriophage screening protocol for teaching in secondary and tertiary institutions. This laboratory practicum was designed to enable students explore the presence of bacteriophages from soil samples. The tendency of many bacteriophages to produce plaques that are small or excessively turbid on agar plates limits their proper visualisation and enumeration. One percent of TTC (2,3,5-Triphenyltetrazolium chloride) was incorporated during the pour plate technique to enhance visibility of bacteriophage plaques on Petri dishes. The isolated bacteriophages were then amplified in growth medium containing targeted bacterial hosts. The results showed presence of six bacteriophages with distinctive plaque morphologies. Students were then taken through bacteriophage amplification and storage procedures. A survey was conducted to evaluate student’s learning experience both prior and after laboratory classes. Analysis from the survey showed that only 26% of students attempted all questions from the pre-laboratory bacteriophage practicum questionnaire whereas 74% failed the survey. On the other hand, 53% of the students attempted all questions for the post-laboratory phage practicum questionnaire and 16% failed the survey.

Bacteriophages applications in agriculture

Humboldt kolleg Ibarra 2019, 2019

The bacteriophages life cycle has two stages: a lytic stage where the phages reproduce inside the bacteria and lyse bacteria and a lysogenic stage where the phage is in a stationary stage where do not exist phage reproduction. The understanding of the life cycle of phages is fundamental to understand the advantages of phage offers as biological control applications and how engineered phages work. The bacteriophages are an alternative to fight against the antimicrobial or pesticides because phages offer advantages such as high host specificity, the ability of long term effect, are active against dividing or not dividing bacterial cells, effective elimination of biofilms and are capable vehicles for nucleic acids delivery. Phages have been isolated from water or soil samples in different parts of the world and for specific bacterial pathogens. In the following review, in the main topics in bacteriophages and its applications in agriculture: the bacteriophages life cycle, advantages of...