Development of Fixed Time Kinetic Spectrophotometric Method for Selective Determination of Metformin in Pharmaceutical Formulations (original) (raw)
Related papers
Indian Journal of Pharmaceutical Education and Research, 2017
Introduction: Diabetes mellitus, a metabolic disorder characterized by increased blood sugar level. Metformin hydrochloride is used to treat type I Diabetes mellitus. Metformin hydrochloride chemically 1, 1-dimethylbiguanide hydrochloride, is white crystalline powder, hygroscopic and freely soluble in water, Officially UV spectrophotometric method used for estimation of Metformin Hydrochloride from the bulk and tablets formulations. Objective: Develop and validate a simple, rapid, accurate, economic and precise UV/VIS method for Metformin Hydrochloride in bulk and tablets formulation. Methodology: Choices of a common solvent were essential so various solvent ranges including methanol, ethanol, acetonitrile and phosphate buffer and various concentrations ranges of various buffers were analyzed. Conclusion: Among different solvents water has showed better results, hence water was selected as a solvent for the proposed method. Metformin Hydrochloride showed maximum absorbance at 234 nm...
Method Development and Validation of Metformin Hydrochloride in Tablet Dosage Form
E-journal of Chemistry, 2011
A simple, reproducible and efficient method for the determination of metformin hydrochloride (MET) was developed and validated. The analysis complied with Beer's law in the concentration range of 8-13 µg/mL at 233 nm for MET. In our study the validation of analytical method for determination of MET by UV in tablets formulation was performed in accordance the parameters including-system suitability, specificity, limit of quantification, limit of detection, linearity of response, accuracy, precision (reproducibility & repeatability), robustness (change of wave length ±2 nm).
Analytica Chimica Acta, 1999
New, simple and convenient potentiometric, spectro¯uorimetric and spectrophotometric methods are described for the determination of metformin in pharmaceutical preparations. The potentiometric technique is based on preparation of PVC membrane sensors incorporating metformin±reineckate, and metformin±tungstosilicate ion-pairs as electroactive species with dioctylphthalate and o-nitrophenyloctylether as plasticizers, respectively. A membrane consisting of carboxylated PVC plasticized with dibutylsebacate is also prepared and tested. These sensors give rapid Nernstian response for 10 À1 ±10 À5 M metformin at pH range 5±11. The metformin±tungstosilicate based sensor is used in a¯ow-through sandwich cell for¯ow injection potentiometric determination of metformin. Graphite sensors coated or doped with metformin±tungstosilicate-PVC, Cu-diethyldithiocarbamate and Ni-diethyldithiocarbamate are also prepared and used for monitoring the titration of the drug with tetraphenyl borate (TPB À), Cu 2 and Ni 2 ions, respectively. The spectro¯uorimetric method depends on the reaction of metformin with chrysenequinone in alkaline medium to give a Schiff's base, which upon hydrolysis gives the free base. The latter in the presence of 1-naphthol gives a¯uorescent product with excitation and emission maxima at 450 and 520 nm, respectively. The¯uorescence±concentration relationship is linear over the range 20±200 mg ml À1 metformin. The proposed spectrophotometric technique involves reaction of metformin with Cu 2 in basic medium to form a Cu±metformin complex. The complex is dissolved in cyclohexylamine and its maximum absorption at 540 nm is measured. Beer's law is obeyed over the range 0.5±2 mg ml À1 metformin. Various cations, some nitrogenous compounds or drug excipients cause no interferences. Results obtained by these techniques are comparable and compare favorably with data obtained using the British Pharmacopoeia method.
A review of analytical techniques for determination of metformin: present and perspectives
International Journal of Health Care and Biological Sciences
Metformin is an oral anti-diabetic drug in preventing complications of type 2 diabetes and it is a good first-line therapy for an over-obese with type 2 diabetes, it is currently available in more than 60 countries worldwide. As a result of the importance of this oral hypoglycaemic agent in the treatment of non-insulin-dependent diabetes mellitus, which leads to end-stage renal disease, this work aims to compile the published analytical methods reported so far in the literature for the determination in biological samples and pharmaceutical formulations. This article narrates different techniques like high-performance liquid chromatography. It can be seen that high-performance liquid chromatography methods have been used extensively. Thus, this paper will help in the selection and development of proper analytical methodologies estimation of Metformin to achieve satisfactory results.
Estimation of Metformin Hydrochloride by UV Spectrophotometric Method in Pharmaceutical Formulation
A simple and sensitive UV spectrophotometric method has been developed and validated for the estimation of metformin hydrochloride in tablet formulation. Metformin hydrochloride is determined spectrophotometrically at 232 nm using distilled water as solvent. It obeyed Beer's law in the range of 2-10 μg/ml. Percentage recovery of the drug for the proposed method ranged from 102-105% indicating no interference of the tablet excipients. The proposed method was found to be accurate and precise for routine estimation of metformin hydrochloride in bulk and pharmaceutical formulation.
International Journal of Pharmaceutical Chemistry, 2014
A simple precise reproducible U.V. Spectrophotometric method have been developed and validated for the simultaneous estimation of MET and GPZ in tablet dosage form. This paper describes the simultaneous equation method as a quantification parameter which involves the measurement of absorbance of MET and GPZ at 237 nm and 234.0 nm respectively. MET and GPZ both obeyed linearity in the range of 2?g/mL to 20 ?g/mL. The recovery studies shows %RSD for MET 0.005, 0.12, 0.65 and for GPZ 0.008, 0.54, 0.25 by SEM method. The results of analysis have been validated statistically for accuracy, Precision, Repeatability, and Ruggedness. The method was successfully applied to the determination of these drugs in pharmaceutical dosage form
A stability indicating RP-HPLC method was developed and validated for the determination of Metformin HCl and Glimepiride in tablet dosage form. The method was carried out using Agilent C18 column (250 mm×4.6 mm, 5 μ) with mobile phase consisting 25 mM Hexane-1-sulphonic acid buffer adjusted to pH 2.5 with ortho-phosphoric acid and acetonitrile (45:55 v/v), at a flow rate of 1.0 mL/min and the effluent monitored at 229 nm. The retention time of Metformin HCl and Glimepiride were found to be 3.55 0.5 and 5.82 0.5 min respectively. Linearity was observed over the concentration range of 150-750 µg/mL for Metformin HCl and 0.75-4.5 µg/mL for Glimepiride. The percentage recoveries of Metformin HCl and Glimepiride in the marketed dosage form found to be 101.6 and 99.9 respectively. The reliability and analytical performance of the proposed method were statistically validated for specificity, linearity, precision, accuracy, and ruggedness, detection, and quantification limits. The drug was subjected to stress conditions including acidic, alkaline, oxidation, photolysis and humidity degradation. The drug is more sensitive towards oxidation degradation. The method was validated as per ICH guidelines.
Chemical Science Transactions, 2014
A novel method to identify the metformin with a good selectivity has been established by using sodium nitroprusside as a chromogenic reagent. The experiment indicates that in basic solution sodium nitroprusside can react with the oxidized product (obtained by the action of sodium hypochlorite) of metformin to form a green colored 1, 3 dinitrosyl-N'-(iminomethyl)-N, N-dimethyl formamidine with maximum absorption at 680 nm. The test when compared with blank is sensitive for spectrophotometric detection of metformin up to 16.56 g in 10 mL solution. The reaction stoichiometric ratio of the oxidized product of metformin to sodium nitroprusside is 1:2. The reaction mechanism of the green product is discussed in present investigation. The proposed method has been successfully applied for identification of metformin in aqueous solution containing glibenclamide, glimepiride, glipizide and gliclazide. Because of the dissociative nature of the green colored product, for a large linear range of microgram concentration of metformin the absorbance of the green colored product is not found to be linear. Hence it can concluded from this analysis that sodium nitroprusside is not suitable chromogenic reagent for spectrophotometric determination of metformin in aqueous solution.
International Journal of Applied Pharmaceutics, 2019
Objective: Area Under Curve method (AUC) and the Multiple Wavelength Spectrophotometric (MWS) method are practice and simple methods for simultaneous assays of Metformin HCl and Glibenclamide on the tablet dosage form. Methods: The AUC method is measured for the absorption spectrum with a concentration 4 mg/l Metformin HCl by calculating the area spectrum at wavelength 230-240 nm and the absorption spectrum with a concentration 8.7 mg/l Glibenclamide by calculating the area at wavelength 225-235 nm. The MWS by determining the absorption spectrum and the five wavelength points for the absorption value at 225 nm, 229.4 nm, 236.6 nm, 233 nm, and 243 nm and calculated using matrix operations. Results: The validation test of the AUC method for Metformin HCl obtained accuracy = 99.35%, linearity = 0.9881, precision = 0.39%, LOD = 0.4459 mg/l. LOQ = 1.4864 mg/l and for Glibenclamide obtained accuracy = 100.79%, linearity = 0.9993, precision = 0.65%, LOD=0.4372 mg/l, LOQ = 1.4072 mg/l and t...
The objective of this study was to develop a simple, efficient, precise and accurate reverse-phase HPLC method for the simultaneous determination of metformin in combination with gliclazide in newly formulated tablets. Chromatographic determination was performed on a reversed phase C18 column (2.6 mm x 250 mm; 5 μm particle size) using a mixture of buffer (1 ml of orthophosphoric acid with 1 ml triethylamine upto 1000 ml with HPLC grade water) and methanol at the ratio of 60:40 as mobile phase at a flow rate of 1ml/min. The UV detection was set at 230 nm. Under the developed conditions, good separation of the analytes was achieved. The calibration curves were linear with coefficient correlation between 0.998 to 1.0 for both drugs over a concentration range of 1 to 50 μg/ml for metformin hydrochloride and 0.16 to 8 μg/ml for gliclazide. The method was also validated in terms of precision (RSD = 0.06 to 3.22%) and accuracy (percent deviation = 0.049 to 2.602%). The proposed method was applied for the analysis of these analytes in newly formulated tablets and potencies were found to be 99.41±0.24% for metformin hydrochloride and 99.77±0.37% for gliclazide which were acceptable.