Pseudomonas rhizosphaerae sp. nov., a novel species that actively solubilizes phosphate in vitro (original) (raw)
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Pseudomonas guariconensis sp. nov., isolated from rhizospheric soil
INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY, 2013
We isolated a bacterial strain designated PCAVU11T in the course of a study of phosphate-solubilizing bacteria occurring in rhizospheric soil of Vigna unguiculata (L.) Walp. in Guárico state, Venezuela. The 16S rRNA gene sequence had 99.2 % sequence similarity with respect to the most closely related species, Pseudomonas taiwanensis , and 99.1 % with respect to Pseudomonas entomophila , Pseudomonas plecoglossicida and Pseudomonas monteilii , on the basis of which PCAVU11T was classified as representing a member of the genus Pseudomonas . Analysis of the housekeeping genes rpoB, rpoD and gyrB confirmed the phylogenetic affiliation and showed sequence similarities lower than 95 % in all cases with respect to the above-mentioned closest relatives. Strain PCAVU11T showed two polar flagella. The respiratory quinone was Q9. The major fatty acids were 16 : 0 (25.7 %), 18 : 1ω7c (20.4 %), 17 : 0 cyclo (11.5 %) and 16 : 1ω7c/15 : 0 iso 2-OH in summed feature 3 (10.8 %). The strain was oxidas...
INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY, 2004
A phosphate-solubilizing bacterial strain designated OK2T was isolated from rhizospheric soil of grasses growing spontaneously in a soil from Spain. Cells of the strain were Gram-negative, strictly aerobic, rod-shaped and motile. Phylogenetic analysis of the 16S rRNA gene indicated that this bacterium belongs to the γ-subclass of Proteobacteria within the genus Pseudomonas and that the closest related species is Pseudomonas graminis. The strain produced catalase but not oxidase. Cellulose, casein, starch, gelatin and urea were not hydrolysed. Aesculin was hydrolysed. Growth was observed with many carbohydrates as carbon sources. The main non-polar fatty acids detected were hexadecenoic acid (16 : 1), hexadecanoic acid (16 : 0) and octadecenoic acid (18 : 1). The hydroxy fatty acids detected were 3-hydroxydecanoic acid (3-OH 10 : 0), 3-hydroxydodecanoic acid (3-OH 12 : 0) and 2-hydroxydodecanoic acid (2-OH 12 : 0). The G+C DNA content determined was 59·3 mol%. DNA–DNA hybridization s...
Pseudomonas bauzanensis sp. nov., isolated from soil
INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY, 2010
A Gram-negative, aerobic, motile rod, designated BZ93T, was isolated from soil from an industrial site. The strain grew at 5–30 °C. Phylogenetic analysis based on 16S rRNA gene sequences showed that strain BZ93T was related to members of the genus Pseudomonas and was related most closely to Pseudomonas xiamenensis C10-2T (97.8 % 16S rRNA gene sequence similarity) and Pseudomonas pertucinogena IFO 14163T (97.4 %). The predominant cellular fatty acids of strain BZ93T were C18 : 1ω7c (54.8 %), summed feature 3 (C16 : 1ω7c and/or iso-C15 : 0 2-OH; 10.3 %), C16 : 0 (9.9 %) and C17 : 0 cyclo (7.4 %). The major quinone was ubiquinone 9. The major phospholipids were phosphatidylethanolamine, diphosphatidylglycerol, phosphatidylglycerol and an unknown phospholipid. The genomic DNA G+C content was 61.8 mol%. On the basis of phenotypic characteristics, phylogenetic analysis and DNA–DNA relatedness, a novel species, Pseudomonas bauzanensis sp. nov., is proposed. The type strain is BZ93T ( = DSM...
TheScientificWorld Journal, 2005
Collections of native Pseudomonas spp. are kept at the NCAM of Uzbekistan. Some of those organisms were isolated from the rhizosphere of cotton, wheat, corn, melon, alfalfa, and tomato grown in field locations within a semi-arid region of Uzbekistan. Strains used for this study were Pseudomonas alcaligenes, P. aurantiaca, P. aureofaciens, P. denitrificans, P. mendocina, P. rathonis, and P. stutzeri. Some of the pseudomonads have been characterized in this report. These strains produced enzymes, phytohormone auxin (IAA), and were antagonist against plant pathogenic fungi in vitro experiments. Most of the strains were salt tolerant and temperature resistant. Some of the Pseudomonas spp. isolated in this study have been found to increase the growth of wheat, corn, and tomato in pot experiments.
International …, 1999
The taxonomic position of a yellow-pigmented group of bacteria, isolated from the phyllosphere of grasses was investigated. Results obtained from restriction analysis of amplified 16s rDNA with seven endonucleases (Cfol, Haelll, Alul, Hinfl, Mspl, Sau3A and ScrFI) showed identical restriction patterns for each enzyme of all isolates studied, which suggests that all strains belong to the same species. The grass isolates displayed the characteristics of the genus Pseudomonas. They were Gram-negative, aerobic and rod-shaped with polar flagella. Isolates were catalase-positive and oxidase-negative, and unable to oxidize or ferment glucose with the production of acid. The isolates did not reduce nitrate to nitrite but were able to utilize a wide range of compounds individually as a sole carbon source, with preference being given to the utilization of monosaccharides. The disaccharides tested were not utilized as substrates. The DNA base compositions of the tested strains ranged from 60 to 61 mol% G+C. The major isoprenoid quinone of each was ubiquinone Q-9 and hydroxy fatty acids were represented b y 3hydroxydodecanoic acid and 2-hydroxydodecanoic acid. Comparison of 165 rDNA sequences showed that the bacteria were members of the genus Pseudomonas, with similarity values between 91.5 and 97.7 O/ O. DNA-DNA hybridization studies with closely related neighbours revealed a low level of homology (< 27 YO), indicating that the isolates represent an individual species. On the basis of phenotypic and phylogenetic analyses a new species, Pseudomonas graminis sp. nov. (type strain DSM 11 3633, is proposed.
Diversity of pseudomonads isolated from three different plant rhizospheres
2001
To study the diversity of the Pseudomonas populations isolated from three different plant rhizospheres, namely pearl millet, cotton and paddy, grown in saline soils along the coastline of Southern India. Methods and Results: The Pseudomonas populations were analysed for their biochemical characters and genetic diversity using molecular tools including RAPD and PCR-RFLP. The biochemical characterization, antibiotic resistance assay and RAPD pro®les revealed a largely homogenous population. Even in PCR-RFLP restriction studies, only two groups of isolates were seen. One group was predominant in all three rhizospheres, while the other minor group consisted of salt-sensitive isolates restricted to the paddy rhizosphere alone. Conclusions: It was observed that increasing salinity caused a predominant selection of salttolerant species, in particular Ps. pseudoalcaligenes and Ps. alcaligenes, irrespective of the host rhizosphere. Signi®cance and Impact of the Study: This study has reinstated the importance of the soil over the host plant with regard to rhizosphere populations. It has also resulted in the isolation of several salt-tolerant Pseudomonas strains, which are being screened for their biological control activity against common plant pathogens of the coastal agri-ecosystem.
International Journal of Systematic and Evolutionary Microbiology, 2017
Within the frame of a biotechnological screening, we isolated two Pseudomonas strains from forest soil. 16S rRNA gene sequence analysis indicated that strain CCOS 864 T shared 99.8 % similarity with Pseudomonas donghuensis HYS T , while strain CCOS 865 T shared 99.0 % similarity with Pseudomonas putida DSM 291 T and lower similarity with other P. putida group type strains. Based on multilocus sequence analysis, the two strains were genotypically distinct from each other, each forming a separate clade. Strains CCOS 864 T and CCOS 865 T were Gram-stain-negative, motile and rod-shaped, growing at a temperature range of 4-37 C. Strain CCOS 864 T could be phenotypically distinguished from P. putida group species by the combination of gelatinase-positive reaction and positive growth on N-acetyl-D-glucosamine, p-hydroxyphenylacetic acid and inosine but lack of fluorescein production on King's B medium, while strain CCOS 865 T could be distinguished from P. putida group species by the combination of positive growth with saccharic acid and negative growth with p-hydroxyphenylacetic acid and L-pyroglutamic acid. The major polar lipid for both strains was phosphatidylethanolamine; the major quinone was ubiquinone Q-9. DNA-DNA hybridization and average nucleotide identities confirmed the novel species status for the two strains. The DNA G+C contents of CCOS 864 T and CCOS 865 T were 62.1 and 63.8 mol%, respectively. The phenotypic, phylogenetic and DNA-DNA relatedness data support the suggestion that CCOS 864 T and CCOS 865 T represent two novel Pseudomonas species. The names Pseudomonas wadenswilerensis sp. nov. (type strain CCOS 864 T =LMG 29327 T) and Pseudomonas reidholzensis sp. nov. (type strain CCOS 865 T =LMG 29328 T) are proposed.
QESSAOUI et al 2019 Applications of new Rhizobacteria Pseudomonas isolates in Agroecology
2019
Pseudomonas isolates have frequently been isolated from the rhizosphere of plants, and several of them have been reported as plant growth-promoting rhizobacteria. In the present work, tomato (Solanum lycopersicum) seeds were germinated in greenhouse conditions, and the seedling height, length of plants, collar diameter and number of leaves were measured from plants grown in soil inoculated by bacterial isolates. Pseudomonas isolates were isolated from the rhizosphere. We used the newman-Keuls test to ascertain pairwise differences. Isolates were identified as a new Pseudomonas species by rpoD gene sequencing. the results showed that isolates of Pseudomonas sp. (Q6B) increased seed germination (P = 0.01); Pseudomonas sp. (Q6B, Q14B, Q7B, Q1B and Q13B) also promoted seedling height (P = 0.01). All five isolates promoted plant length and enlarged the collar diameter (P = 0.01). Pseudomonas sp. (Q1B) also increased leaf number (P = 0.01). The investigation found that Pseudomonas isolates were able to solubilize phosphate, produce siderophores, ammonia, and indole-3-acetic acid and colonize the roots of tomato plants. This study shows that these five novel Pseudomonas sp. isolates can be effective new plant growth-promoting rhizobacteria.
Pseudomonas punonensis sp. nov., isolated from straw
INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY, 2013
During a study of the &amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;#39;tunta&amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;#39; (frozen-dry potato) production process in Peru, a bacterial strain, LMT03(T), was isolated from the straw grass in which the potatoes are dried. This strain was classified into the genus Pseudomonas on the basis of the 16S rRNA gene sequence analysis, and is most closely related to Pseudomonas argentinensis CH01(T) with 99.3 % identity in this gene and 96 %, 92 % and 86 % identities in rpoB, rpoD and gyrB genes, respectively. Strain LMT03(T) has a single polar flagellum, like other related yellow-pigment-producing pseudomonads. The major quinone is Q-9. The major fatty acids are C18 : 1ω7c in summed feature 8 (40.82 %), C16 : 1ω6c/C16 : 1ω6c in summed feature 3 (23.72 %) and C16 : 0 (15.20 %). The strain produces oxidase but it does not produce gelatinase, indole, urease, arginine dihydrolase or β-galactosidase. Catalase production was very weak after 28 and 48 h incubation on nutrient agar medium. Nitrate reduction is negative. It does not hydrolyse aesculin. The DNA G+C content is 57.8 mol%. DNA-DNA hybridization results showed lower than 52 % relatedness with respect to the type strain of P. argentinensis, CH01(T). These results, together with other phenotypic characteristics, support the definition of a novel species within the genus Pseudomonas, for which the name Pseudomonas punonensis sp. nov. is proposed. The type strain is LMT03(T) ( = LMG 26839(T) = CECT 8089(T)).