Effect of in ovo Injection of L-Arginine in Different Chicken Embryonic Development Stages on Post-hatch Growth Performance, Immune Response and Muscle Development Proteins (original) (raw)
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Jurnal Ilmu Ternak dan Veteriner
This study aimed to determine the effect of in-ovo injection of L-arginine on hatchability, chick quality, performances, and muscle histology of native chicken. In-ovo injection was carried out on the 10th day. A total of 375 fertile eggs with an average weight ranged 39-43 g were grouped into 5 treatments. The first treatment was without injection (negative control), the second treatment was injection of NaCl solution 0.9% (positive control), the third treatment was injection of L-arginine solution 0.5 g per 100 ml of NaCl 0.9% (0.5%, m/v) The fourth treatment was injection of 1.0 g L-arginine solution per 100 ml of NaCl 0.9% (1.0%, m/v), and the fifth treatment was injection of 1.5 g L-arginine solution per 100 ml of NaCl 0.9% (1.5%, m/v). The injection was carried out at the pointed area of the egg in a vertical position (pointed below, blunt above). The Injection was carried out with a depth of 10 mm from the eggshell using an automatic injector. The results showed that all tre...
Buletin Peternakan
This study aims to determine the effect of in-ovo injection of L-arginine into local chicken eggs on embryo development and chick performance of local chicken. A total of 160 eggs were incubated using semi-automatic incubator with temperature of 37-38C and relative humidity of 55-65%. The injected L-arginine solution has a concentration of 0.5% (m/v). In treatment was divided into 4 groups, the first treatment was without injection (control), the second treatment was the injection of 0.2 mL L-arginine solution 0.5% (m/v)/egg, the third treatment was the injection of 0.4 mL L-arginine solution. 0.5% (m/v)/egg, and fourth treatment was the injection of L-arginine solution 0.6 mL 0.5%(m/v)/egg. The results showed that embryo mortality and hatchability were lower with L-arginine injection treatment than without injection. All treatments showed no effect on extraembryonic fluid absorption, but there was an increase in embryo weight in the injection treatment 0.2, 0.4, 0.6 mL L-Arginine 0...
Journal of Animal Physiology and Animal Nutrition, 2019
Recently, nutrient manipulations have been used to improve the birds' immune system (Adibmoradi et al., 2015). Arginine considered an essential amino acid in broiler chickens due to their higher growth rate and the lack of functional urea cycle in birds (Khajali & Wideman, 2010). l-arginine is also a precursor for the biosynthesis of essential molecules, included: proteins, ornithine, polyamines, nitric oxide, glutamate, glutamine, proline and creatine, which are necessary for growth, immunity and disease resistance of birds (Khajali & Wideman, 2010). Previous researches indicated the improving effect of higher dietary arginine levels on feed efficiency, growth performance, muscle production, weight of lymphoid organs, lymphocyte proliferation, antibody titres to Newcastle disease, skin reaction to phytohemagglutinin P, and concentrations of interferon (IFN)-α, IFN-γ, immunoglobulin (Ig) G, IgA and IgM, while reducing
The present study aimed to evaluate the effect of dietary supplementation with different levels of L-arginine (L-Arg) above the levels recommended by NRC (1994) on egg production, reproduction performance, egg quality and some physiological and immunological parameters. The experimental period started when the hens were 40 week old, and ended to three months. Two local strains Fayoumi(Fa) and Golden Montazah(GM) were used with a total number of 180 laying hens and 18 males (90 females and 9 males from each strain). Selected male and female of each strain were divided randomly into 3 groups/strain, each group consisted of 3 replicates (10 female and 1 male /replicate). Control diet (contain recommended level of L-Arg0.700% L-Arg). Crystalline amino acid (L-Arg) was supplemented by 2% and 4% as a percentage of dietary L-Arg to achieve 0.714% L-Arg and 0.728% L-Arg respectively (treatment groups).During the experimental period thirty six cockerels (18 from each strain) were housed in individual cage; each 6 cockerels fed one of the experimental diets to determine semen quality. During the experimental period, feed consumption and egg number were recorded daily. Eggs were weighed daily and egg mass per hen per day were calculated. Feed conversion ratio and crude protein conversion were calculated. During the last month of the experiment three hatches were conducted weekly to determine hatchability parameters. At the end of the experiment random samples of eggs representing controls and treatments (10 eggs /replicate) for each strain examined for egg quality parameters. Two blood samples were collected from each replicate within each strain in all groups to estimate physiological and immunological parameters. Experimental results showed that: Arginine supplementation above NRC caused significant increase in egg production percent and egg mass during the whole experimental period. There was at least 6% of extra egg production over the control.
The Indian journal of animal sciences
albumen and yolk contain amino acids for the developing embryo, however, a large fraction of egg protein consists of antibodies, which the hen produces during the immune response experienced at the time of egg laying (Losch et al. 1986). Under normal circumstances, these immunoglobulins are fully functional at the time of hatch (Brierley and Hemmings 1956). Gluconeogenesis from protein provides glycogen that fuels hatching activities (Klasings 1998). Thus, early provision of nutrients affects immediate embryo survival and disease resistance and also the ultimate attainment of genetic potential. Efforts were made to achieve higher protein synthesis by injecting AA directly into the egg (Ohta et al. 2001, Bhanja et al. 2004b). Accelerated enteric development and improved nutritional status afforded by in ovo feeding improved hatching weight, growth rate (Al-Murrani 1982, Ohta et al. 1999 and Bhanja et al. 2004b), immune responses (Konashi et al. 2000, Bhanja and Mandal 2005), gastro-i...
Nigerian Journal of Animal Production
In the study, the effects of in ovo injection of amino acids (L-methionine, L-lysine, and Larginine) and their combinations on hatching traits, post-hatch performance, cell-mediated immune response and blood profile of FUNAAB Alpha broiler chicken were evaluated. The study was carried out in two trials. In the first trial, a total of 360 hatching eggs of indigenous chicken were fumigated, weighed and placed in the incubator. On day 14 of incubation, candling was done and eggs with living embryos were distributed into four treatments; Control (un-injected eggs), L-methionine injected eggs, L-arginine injected eggs and L-lysine injected eggs. Each egg was injected 0.5 mL solution of the amino acid on day18. Hatched chicks were distributed into three replicates containing 30 chicks each. In trial 2, another 360 hatching eggs were used in the treatments which were; Control (eggs without amino acid injection), L-arginine injected eggs, combination of L-arginine and L-methionine injected ...
Animals
Sulfur amino acids are typically the first-limiting amino acids (AA) used in protein metabolism in poultry. Therefore, we hypothesized that their utilization in the pre-hatch period would affect embryonic development, IGF-I and TLR4 gene expression, antioxidant status, serum biochemical profile, and jejunum histomorphometry of newly hatched Ross broiler chicks incubated under heat stress conditions. A total of 150 fertile broiler eggs were subjected to heat stress (39.6 °C for 6 h/d) from d10 until d18 and injected at d 17.5 of incubation with methionine and cysteine (Met-Cys) at a dose of 5.90 mg l-methionine plus 3.40 mg l-cysteine. The effects of Met-Cys administration were examined and compared with the control (Non-injected group) and 0.75% NaCl injected group. The results showed that no significant differences among all groups in serum protein profiles (total protein, albumin, globulin, and albumin/globulin ratio) and creatine kinase were observed. The level of heat shock prot...
Effects of in ovo administration of amino acids on hatchability and performance of meat chickens
South African Journal of Animal Science, 2014
The effects of in ovo amino acid administration on hatchability traits and post-hatch performance were studied using eggs from a meat-type breeder flock (Ross) at 38 weeks of age. On day 15 of incubation, five treatments were applied, consisting of six replicates with 16 eggs per replicate. The treatments consisted of a non-injected negative control; a positive control in which saline was injected; and three treatments with saline, containing 23.72 mg lysine, glutamine, glycine and proline (AA1); 23.60 mg arginine, glutamine, glycine and proline (AA2); and 28.76 mg lysine, arginine, glutamine, glycine and proline (AA3). The hatchability traits were incubation period, chick weight at hatch as an absolute value (CWT) and chick weight at hatch as a percentage of egg weight (CWTP), and the post-hatch performance traits were body weight gain (BWG), feed intake (FI) and feed conversion ratio (FCR). Measurements on the chickens were taken during the starter (1 -21 days of age), finisher (22 -35 days of age) and entire (1 -35 days of age) experimental periods. In ovo amino acid treatments improved CWTP without affecting hatchability traits, and increased FI without altering the FCR of chickens during the entire experimental period. Chickens of the in ovo AA1 treatment had a higher FI than those of the control treatments during the finisher period. In ovo AA2 treatment increased CWT and BWG of the chickens during the starter period and entire experimental period and FI during the finisher period, when compared with those of the control treatments. In ovo AA2 treatment resulted in a longer period of incubation than those of other treatments. It was concluded that in ovo amino acid treatment increased CWTP and FI of chickens without affecting hatchability traits and feed efficiency and in ovo of AA2 treatment increased the incubation period, CWT and BWG when compared with those of control treatments during the entire experimental period. ______________________________________________________________________________________
Role of Amino Acid Arginine for Broiler Production: A Review
Journal of Engineering and Science Research
Amino acids are known as anabolic factors that are essential for formation of muscle by stimulating protein synthesis while inhibiting proteolysis, and they are significant component for the synthesis of various nitrogenous compounds. There are 20 amino acids are essential to require in cell for formation of body protein of which about 10 amino acids, which cannot be synthesized by the birds are termed essential. Among the essential amino acid arginine one of the essential amino acids for chickens because, like other birds, they are unable to obtain Arginine from endogenous sources due to the absence of most of the enzymes involved in the urea cycle. This amino acid involved in synthesis of proline, hydroxyl proline and polyamines which are essential for connective tissue synthesis as well as increased growth of chicken. Moreover, L-arginine (L-Arg) is effective for reducing fat deposition in broiler. Moreover, it decrease heat stress increase meat quality and increase immune respon...