Chemoprotective effect of nanocurcumin on 5-fluorouracil-induced-toxicity toward oral cancer treatment (original) (raw)
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Journal of maxillofacial and oral surgery, 2018
The chemotherapeutics agent, 5-fluorouracil (5-FU), and curcumin (Cur), a natural antioxidant, has a wide pharmacological window to treat oral carcinoma; however, both drugs have limited bioavailability. This research study designs to develop a nanoemulsions (NEs) formulation by combining 5-FU and Cur to improve anticancer activity against oral cavity squamous cell carcinoma (OSCC) cells from the diversified origin for in vitro analysis, SCC090 (human tongue) and SCC152 (human hypo-pharynx). NEs formulated through homogenization, applying high-energy ultrasonication technique. The prepared 5-FUNE/Cur-NE/5-FU-Cur-NE were characterized and optimized by different in vitro assays to evaluate release system and treatment of OSCC cells to monitor cellular acceptability, such as in vitro anticancer activity by MTT assay, cell uptake studies and protein expression associated apoptotic study. 5-FUNE/Cur-NE/5-FU-Cur-NE successfully formulated and show mean-value of the particle size (150-200 ...
International journal of oncology, 2013
Curcumin is a polyphenolic compound which possesses anticancer potential. It has been shown to induce cell death in a variety of cancer cells, however, its effect on CAL27‑cisplatin-resistant human oral cancer cells (CAR cells) has not been elucidated to date. The low water solubility of curcumin which leads to poor bioavailability, however, has been highlighted as a major limiting factor. In this study, we utilized water-soluble PLGA curcumin nanoparticles (Cur-NPs), and investigated the effects of Cur-NPs on CAR cells. The results showed Cur-NPs induced apoptosis in CAR cells but exhibited low cytotoxicity to normal human gingival fibroblasts (HGFs) and normal human oral keratinocytes (OKs). Cur-NPs triggered DNA concentration, fragmentation and subsequent apoptosis. Compared to untreated CAR cells, a more detectable amount of Calcein-AM accumulation was found inside the treated CAR cells. Cur-NPs suppressed the protein and mRNA expression levels of MDR1. Both the activity and the...
Use of nanosystems to improve the anticancer effects of curcumin
Beilstein Journal of Nanotechnology, 2021
Curcumin (CUR) is a phenolic compound that is safe for human consumption. It exhibits chemopreventive, antiproliferative, antiangiogenic, and antimetastatic effects. However, these benefits can be hampered due to the lipophilic nature, rapid metabolism, low bioavailability, and fast elimination of the molecule. Considering this, the present work reviews the use of CUR-based nanosystems as anticancer agents, including conventional nanosystems (i.e., liposomes, nanoemulsions, nanocrystals, nanosuspensions, polymeric nanoparticles) and nanosystems that respond to external stimuli (i.e., magnetic nanoparticles and photodynamic therapy). Previous studies showed that the effects of CUR were improved when loaded into nanosystems as compared to the free compound, as well as synergist effects when it is co-administrated alongside with other molecules. In order to maximize the beneficial health effects of CUR, critical factors need to be strictly controlled, such as particle size, morphology,...
Selective Cytotoxic and Chemoprotective effect of nanocurcumin against human HCT-116 cell line
Biochemistry Letters, 2018
Background: limitation of curcumin use as antioxidant and anticancer may related to its low bioavailability so its use as nanocurcumin may improve its effect. Aim: this study was designed to investigate the possible chemoprotective and cytotoxic effect of nanocurcumin against Human colorectal carcinoma (HCT-116) cell line Material and methods: MTT assay was performed to detect the cytotoxic effect of nanocurcumin against normal and HCT-116 cells, and determination of gene expression of P53, BAX, Caspase-9 and BCL-2 was performed to detect the apoptotic effect of nanocurcumin Results: nanocurcumin has a selective cytotoxic effect on human HCT-116 cells with no cytotoxic effect on healthy cells. At the same time, nanocurcumin increases the expression of P53, BAX and Caspase-9 with reduction in expression of BCL-2 gene in medium and high doses with no effect in lower dose Conclusion: nanocurcumin has selective cytotoxic and chemoprotective effect against human HCT-116 cells according to its dose used.
In vitro and in vivo studies on the anticancer potential of curcumin and nanocurcumin
2020
Purpose: Curcumin, a polyphenolic compound that obtained from the herb of Curcuma longa, has many anticancer effects. But, its effect is low due to poor water solubility. In order to improve its solubility and drug delivery, we have utilized a nano-curcumin. Methods: In vitro cytotoxicity and anti-tumor promoting effects of nanocurcumin and normal curcumin were investigated. Results revealed that nanocurcumin is able to inhibit the growth of two human cancer cell lines Hep-G2 and HCT116 with IC50 values of 5.68 and 6.53 μg ml−1, respectively,while free curcumin expresses the activity with IC50 values of 8.28 and 9.64μg ml−1. At the concentration of 40 μg ml−1. Nanocurcumin showed antitumor promoting effects in reducing tumor size by 59.8 % , while the percentages caused by curcumin was 41.4%, respectively. Mice were treated with equal concentration of nanocurcumin and curcumin showed an improvement in the antioxidant and anti-inflammatory cytokines. The level of improment in EC bear...
Chemico-Biological Interactions, 2012
Toxic outcome of chemical therapeutics as well as multidrug resistance are two serious phenomena for their inacceptance in cancer chemotherapy. Antioxidants like curcumin (Cur) have gained immense importance for their excellent anticarcinogenic activities and minimum toxic manifestations in biological system. However, Cur is lipophilic and thus following oral administration hardly appears in blood indicating its potential therapeutic challenge in cancer therapy. Nanocapsulated Cur has been used as a drug delivery vector to focus the effectiveness of these vesicles against hepatocellular carcinoma. The theme of work was to evaluate effectiveness in oral route of polylactide co-glycolide (PLGA) Nanocapsulated curcumin (Nano Cur) against diethylnitrosamine (DEN) induced hepatocellular carcinoma (HCC) in rat. Nano Cur of average diameter 14 nm and encapsulation efficiency of 78% were prepared. Fourier Transform Infra Red (FTIR) analysis revealed that there is no chemical interaction between drug and the polymer. Three i.p. injections of the chemical hepatocarcinogen DEN at 15 days interval causes hepatotoxicity, the generation of reactive oxygen species (ROS), lipid peroxidation, decrease in plasma membrane microviscosity and depletion of antioxidant enzyme levels in liver. Nano Cur (weekly oral treatment for 16 weeks at 20 mg/kg b.wt) in DEN induced HCC rats exerted significant protection against HCC and restored redox homeostasis in liver cells. Nanocapsulated Cur caused cancer cell apoptosis as visualized by ApoBrdU analysis. Histopathological analysis confirmed the pathological improvement in the liver. Nano Cur was found to be a potential formulation in oral route in combating the oxidative damage of hepatic cells and eliminating DEN induced hepatocellular cancer cells in rat whereas identical amount of free Cur treatment was found almost ineffective.
Nanocurcumin in Oral Squamous Cancer Cells and Its Efficacy as a Chemo-Adjuvant
Cureus, 2022
Oral squamous cell carcinoma is the sixth most common cancer worldwide. Despite the available treatment, the survival rate is poor. The addition of agents to make chemotherapeutics safer and more effective is important. Curcumin is a common Indian spice that has shown anticarcinogenic properties. It has been possible to overcome its poor bio-availability using nanotechnology. We aimed to investigate the adjuvant effect of nanocurcumin (NC ~ 200 nm size) treatment on cetuximab (epidermal growth factor receptor inhibitor) in oral squamous cancer cells (KB 3-1 cell). Cancer cells were cultured and treated for 24 hours with cetuximab and NC, in various doses to find the drugs' half-maximal inhibitory concentration (IC 50). Experiments were conducted with a combination dose of both and sensitization treatment with NC before cetuximab with cytotoxicity assessment by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay. One-way analysis of variance (ANOVA) was used to compare different treatment groups. We found a concentration-dependent cancer cell death with NC, which was significant compared to cetuximab (p <0.001). The combination treatment group had highly significant cell death (p <0.0001) compared to a single drug, and the NC sensitization caused substantial cell death compared to a single cetuximab treatment (p<0.01). Our study findings indicate the potential chemo-adjuvant effect of NC in oral cancer.
Cytotoxic effects investigation of nanomicelle and free curcuminoids against cancer and normal cells
Curcumin, which is derived from the turmeric rhizomes (curcuma longa) as a natural polyphenol, is a substantially lipophilic molecule. This commonly used substance is employed as a coloring agent and spice in food and contains potent antioxidant, as well as anti-inflammatory, and anti-proliferative tumor activities. The developed nanomicelle formulations of curcumin are used to promote the bio-availability and solubility of the above-mentioned lipophilic molecule. The present investigation aimed to examine the anti-proliferative activity of nanomicelle and free curcuminoids by using different cancer and normal cells using a tetrazolium dye-based assay. To this end, various cell lines were treated with nanomicelle or free curcuminoids at different concentration of 5, 10, 20, 30, and 40 µM for 48 hours at 37 o C. Our results demonstrated that the half maximal inhibitory concentrations of the micellar form of curcuminoids for different cancer cell lines were as high as its levels measu...
Utilization of curcumine and nanocurcumine compounds in cancer therapy
Pharmaceutical and Biomedical Research, 2017
Curcumin is a polyphenol extracted from the Curcuma plant. Curcumin has been used widely in ayurvedic medicine for centuries; it has a variety of biological properties including anti-oxidant, analgesic, anti-inflammatory and antiseptic activity. Curcumin has shown anti-cancer activities through variety of biological pathways engaged in mutagenesis, oncogene expression, cell cycle regulation, apoptosis, tumorigenesis and metastasis. Curcumin has proved anti-proliferative effect in many cancers, and is an inhibitor of the transcription factor NF-κB and downstream gene products (including c-myc, Bcl-2, COX-2, NOS, Cyclin D1, TNF-α, interleukins and MMP-9). Furthermore, curcumin affects a variety of growth factor receptors and cell adhesion molecules involved in tumor growth, angiogenesis and metastasis. However, a limiting factor is its extremely low bioavailability which hinders its use as therapeutic agent. Therefore, many technologies have been developed to overcome this limitation. We summarize to develop curcumin delivery aims and increase solubility for improving curcumin bioavailability and anticancer potential for therapy.
BioTechnologia
Nanotechnology holds significance in all fields of research, and the formation and surface alterations of nanomaterials are particularly important in this discipline. Nanoformulations synthesized with bioactive plant components play a crucial role in the improvement of several therapeutics and diagnostics. In the present study, we reported the synthesis of a curcumin nanoformulation (CN) by using curcumin and D-α-tocopheryl polyethylene glycol 1000 succinate (TPGS). The synthesized CN was characterized using dynamic light scattering, UV-Visible spectrophotometry, Fourier-transform infrared spectroscopy, field-emission scanning electron microscopy, and X-ray diffraction. Furthermore, it was evaluated for solubility, drug loading, encapsulation efficiency, stability, in vitro release, and anticancer potentials. The role of TPGS in the synthesis of CN was validated. The synthesized CN exhibited a size of 6.2 ± 1.9 nm, needle-shaped morphology, a polydispersity index of 0.164, and zeta potential of !10.1 ± 3.21 mV, as determined by characterization techniques. Its water solubility was 2.5 × 10 4 times higher than that of pure curcumin. The encapsulation efficiency and curcumin loading efficiency of the synthesized CN were found to be 80 and 10%, respectively, with storage stability exceeding 30 days. Moreover, the synthesized CN demonstrated significant in vitro anticancer activity against the colorectal cancer cell line HCT-116, with an IC50 value of 12.74 ± 0.54 μM at 24 h.