Rapid Detection of Hybrid Maize Parental Lines Using Stacking Ensemble Machine Learning (original) (raw)

Identification of haploid and diploid maize seeds using convolutional neural networks and a transfer learning approach

Computers and Electronics in Agriculture, 2019

Maize is one of the most significant grains cultivated all over the world. Doubled-haploid is an important technique in terms of advanced maize breeding, modern crop improvement and genetic programs, since this technique shortens the breeding period and increases breeding efficiency. However, the selection of the haploid seeds is a major problem of this breeding technique. This process is frequently conducted manually, and this unreliable situation leads to loss of time and labor. Inspired by the recent successes of deep transfer learning, in this study, we approached this problem as a computer vision task to provide a nondestructive, rapid and low-cost model. To achieve this objective, we adopted convolutional neural networks (CNNs) to recognize haploid and diploid maize seeds automatically through a transfer learning approach. More specifically, AlexNet, VVGNet, GoogLeNet, and ResNet were applied for this specific task. The experimental study was carried out using a new dataset consisting of 1230 haploid and 1770 diploid maize seed images. The samples in the dataset were classified considering a marker-assisted selection, known as the R1-nj anthocyanin marker. To measure the success of the CNN models, we utilized several performance metrics, such as accuracy, sensitivity, specificity, quality index, and F-score derived from the confusion matrix and receiver operating characteristic curves. According to the experimental results, the CNN models ensured promising results, and we achieved the most efficient results via VGG-19. The accuracy, sensitivity, specificity, quality index, and F-score of VGG-19 were 94.22%, 94.58%, 93.97%, 94.27%, and 93.07%, respectively. Consequently, the experimental results proved that CNN models can be a useful tool in recognizing haploid maize seeds. Furthermore, we conclude that this approach is significantly superior to machine learning-based methods and conventional manual selection.

Maize Kernel Abortion Recognition and Classification Using Binary Classification Machine Learning Algorithms and Deep Convolutional Neural Networks

AI

Maize kernel traits such as kernel length, kernel width, and kernel number determine the total kernel weight and, consequently, maize yield. Therefore, the measurement of kernel traits is important for maize breeding and the evaluation of maize yield. There are a few methods that allow the extraction of ear and kernel features through image processing. We evaluated the potential of deep convolutional neural networks and binary machine learning (ML) algorithms (logistic regression (LR), support vector machine (SVM), AdaBoost (ADB), Classification tree (CART), and the K-Neighbor (kNN)) for accurate maize kernel abortion detection and classification. The algorithms were trained using 75% of 66 total images, and the remaining 25% was used for testing their performance. Confusion matrix, classification accuracy, and precision were the major metrics in evaluating the performance of the algorithms. The SVM and LR algorithms were highly accurate and precise (100%) under all the abortion sta...

Machine Learning for Crop Science: Applications and Perspectives in Maize Breeding

Revista Brasileira de Milho e Sorgo

Machine learning (ML) has been a major driver in complex data analysis in recent decades, allowing the mining of large databases. ML techniques allow the creation of computational models for prediction, pattern extraction and recognition, considering the premise that the computer acquires learning skills to perform a given task without being explicitly programmed for such a purpose. Driven by the efficiency of these techniques, several studies have demonstrated their wide range of applications and high potential for maize breeding. From the prediction of genetic values by omic data to applications of high-throughput phenotyping data, ML models have promoted advances in the species comprehension and assisted in the development of more effective tools for its breeding, driving expressive yield gains. In this context, this work presents the main contributions of ML in maize breeding, providing a broad view of the main studies and methodological perspectives in the area.

Applications of Machine Learning for Maize Breeding

Corn is one of the world's most important cereals and a major source of calories for humanity, along with rice and wheat. Climate change and the use of marginal land for crop production requires the development of genotypes adapted to stressful environments, particularly drought tolerant plants. Among the new tech-nologies currently available for accelerate the releasing of new genotypes there is an emerging discipline called Machine Learning (ML). A primary goal of ML algo-rithms is to automatically learn to recognize complex patterns and make intelligent decisions based on data. This work reviews several strategic applications of ML in maize breeding. Quantitative trait loci mapping, heterotic group assignment and the popular genome-wide selection are some of the key areas currently addressed by the literature. Results are encouraging and propose ML algorithms as a valuable alter-native to traditional statistical techniques applied in maize, even the more recently introduced l...

Supervised machine learning and heterotic classification of maize ( Zea mays L.) using molecular marker data

Computers and Electronics in Agriculture, 2010

The development of molecular techniques for genetic analysis has enabled great advances in cereal breeding. However, their usefulness in hybrid breeding, particularly in assigning new lines to heterotic groups previously established, still remains unsolved. In this work we evaluate the performance of several state-of-art multiclass classifiers onto three molecular marker datasets representing a broad spectrum of maize heterotic patterns. Even though results are variable, they suggest supervised learning algorithms as a valuable complement to traditional breeding programs.▶ Molecular techniques have been proposed to assign maize inbreds to heterotic groups. ▶ We evaluate several supervised learning algorithms onto 3 maize datasets. ▶ Results suggest multiclass classifiers as an alternative to traditional statistical methods.

A decision support system for hybrid corn classification

IOP Conf. Series: Earth and Environmental Science 911 (2021) 012033, 2021

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Maize seed variety identification model using image processing and deep learning

Institute of Advanced Engineering and Science (iaes), 2024

Maize is Ethiopia's dominant cereal crop regarding area coverage and production level. There are different varieties of maize in Ethiopia. Maize varieties are classified based on morphological features such as shape and size. Due to the nature of maize seed and its rotation variant, studies are still needed to identify Ethiopian maize seed varieties. With expert eyes, identification of maize seed varieties is difficult due to their similar morphological features and visual similarities. We proposed a hybrid feature-based maize variety identification model to solve this problem. For training and testing the model, images of each maize variety were collected from the adet agriculture and research center (AARC), Ethiopia. A multi-class support vector machine (MCSVM) classifier was employed on a hybrid of handcrafted (i.e., gabor and histogram of oriented gradients) and convolutional neural network (CNN)-based feature selection techniques and achieved an overall classification accuracy of 99%.

Computer-Aided Multiclass Classification of Corn from Corn Images Integrating Deep Feature Extraction

Computational Intelligence and Neuroscience

Corn has great importance in terms of production in the field of agriculture and animal feed. Obtaining pure corn seeds in corn production is quite significant for seed quality. For this reason, the distinction of corn seeds that have numerous varieties plays an essential role in marketing. This study was conducted with 14,469 images of BT6470, Calipso, Es_Armandi, and Hiva types of corn licensed by BIOTEK. The classification of images was carried out in three stages. At the first stage, deep feature extraction of the four types of corn images was performed with the pretrained CNN model SqueezeNet 1000 deep features were obtained for each image. In the second stage, in order to reduce these features obtained from deep feature extraction with SqueezeNet, separate feature selection processes were performed with the Bat Optimization (BA), Whale Optimization (WOA), and Gray Wolf Optimization (GWO) algorithms among optimization algorithms. Finally, in the last stage, the features obtaine...

Classification of hybrid maize seeds (Zea mays) with object-based machine learning algorithms using multispectral UAV imagery

6th Intercontinental Geoinformation Days, 2023

In recent years, detailed monitoring of different vegetation classes by using modern remote sensing technologies has become one of the essential issues for smart agriculture activities. In this study, using three advanced machine learning algorithms, namely canonical correlation forest (CCF), rotation forest (RotFor) and support vector machines (SVM), and object-based image classification techniques on multispectral (MS) unmanned aerial vehicle (UAV) orthomosaics, the separability of 12 maize species were investigated. The investigations were performed in Sakarya Maize Research Institute application area located in Arifiye district of Sakarya province, Turkey. In maize monitoring, besides the five spectral bands (R, G, B, red edge, NIR) of the MS UAV, the Normalized Digital Surface Model (NDSM) describing the height of maize species was generated and included as an additional band to improve classification performance, evaluated with F-score, overall accuracy (OA) and Kappa metrics. The results demonstrated that CCF and RotFor algorithms provide similar OA as 76.61% and 76.75%, respectively and the SVM algorithm has 74.18%. In parallel, the Kappa values of CCF and RotFor are 0.75 and the SVM is 0.72. In terms of class-based F-scores, by all algorithms, C. Sweet and C. Arifiye were identified with over 97% and 94% accuracies, respectively, that prove the successful determination of their boundaries using object-based classification.