Level of Expression of the Tomato rbcS-3A Gene is Modulated by a Far Upstream Promoter Element in a Developmentally Regulated Manner (original) (raw)
1989, THE PLANT CELL ONLINE
By Agrobacterium-mediated transformation we have demonstrated that a 1.10-kilobase promoter sequence from the tomato rbcS-3A gene confers light-inducible and organ-specific expression upon fusion to the bacterial chloramphenicol acetyltransferase gene. A biphasic expression profile was obtained by 5" deletion analysis of this promoter, indicating the presence of both positive and negative regulatory elements. A severe reduction in the level of expression was observed when the 5'-terminal 90 base pairs were deleted from the 1.10-kilobase promoter. DNA sequence elements responsible for light inducibility and organ specificity of the gene reside within the-374 base pairs of the proximal part of the promoter and the sequences spanning from-374 to-205 are essential for promoter function. The DNA sequences upstream from-374 modulate the level of expression in leaf tissue; this modulation is under developmental control.
Sign up for access to the world's latest research.
checkGet notified about relevant papers
checkSave papers to use in your research
checkJoin the discussion with peers
checkTrack your impact
Loading Preview
Sorry, preview is currently unavailable. You can download the paper by clicking the button above.