Analysis of glycated hemoglobin A1c by capillary electrophoresis and capillary isoelectric focusing (original) (raw)

Two capillary electrophoretic methods were developed and evaluated for measurement of glycated hemoglobin A1c (HbA1c). First, a capillary electrophoresis analysis is performed with a sodium tetraborate buffer (pH 9.3) as background electrolyte in a neutrally coated capillary. HbA1c is separated from HbA0 due to specific interactions of borate anions with the cis-diol pattern in the saccharide moiety of glycohemoglobin. Second, a capillary isoelectric focusing method exploits a difference in pI values of HbA0 and HbA1c, using Servalyt pH 6-8 or Biolyte pH 6-8 carrier ampholytes. Both methods reflect recent technological advancements and provide accurate quantification of HbA1c, aligning well with previous electrophoretic assays.