Molecular study to detect the Eimeria species in sheep in Al-Diwaniyah province, Iraq (original) (raw)
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International Journal of Livestock Research, 2014
The present study was carried out to survey the prevalence and intensity of coccidia in sheep in western Iran. The prevalence of coccidial infection was monitored in 410 sheep in western Iran between March 2012 to February 2013. Oocysts in faecal samples were detected using a flotation technique, sporulated and identified to species based on morphological features of the sporulated oocysts. The overall prevalence of coccidial infection was 91.5% (375/410
The prevalence of ovine Eimeria infection in Rudsar, North of Iran, (2011–2012)
Journal of Parasitic Diseases, 2014
This study was undertaken to determine the prevalence of Eimeria infection in sheep in Rudsar, North of Iran, in period of 1 year (March 2011 to March 2012). The samples (approximately 3-5 grams of faeces) were collected from the rectum of 270 sheep and transferred to the parasitology laboratory. Eimeria species were identified following sporulation of faeces in a thin layer of 2.5 % potassium dichromate for one or 2 weeks at 27°C. Out of 270 faecal samples examined, 170[(63.0 %; 95 % Confidence Interval (CI) 56.9-68.7)] were positive for Eimeria spp. Five species of Eimeria, including E. ahsata, E. ovina, E. crandallis, E. parva and E. ovinoidalis were identified in faecal samples by modified McMaster technique. The most frequent species was E. crandallis. Sheep were considered in four age groups (less than 1 year old, 1-2 years old, 2-3 years old and over 3 years old).The results also indicated that less than 1 year old sheep have greater infection ratio as compare with older animals. Female had significantly higher prevalence of Eimeria than male. Higher infection was seen in females compare to males and in winter compare to summer. Parasitological gross and microscopic examinations revealed Eimeria infection is common in sheep of Rudsar, North Iran.
Occurrence and diversity of Eimeria species in cattle in Hamedan province, Iran
Veterinární Medicína, 2014
Information on the occurrence, diversity and economic losses due to Eimeria infection on cattle farms is lacking in Western Iran. The principal aim of this study was to determine the prevalence and diversity of Eimeria species in cattle in Hamedan province, Western Iran. In a cross-sectional study performed from June 2010 to April 2013, 400 faecal samples were collected randomly from animals without clinical signs from different cattle farms of Hamedan province and examined for Eimeria species infection using parasitology methods. The overall infection rate of Eimeria species was 8.25%. Nine species of Eimeria were identified including: E. bovis (23.7%), E. zuernii (19.2%), E. canadensis (12.6%), E. ellipsodalis (11.4%), E. alabamensis (10.4%), E. pellita (9.1%), E. auburnensis (6.8%), E. cylindrica (4.6%) and E. bukidnonensis (2.3%). There were statistically significant differences with respect to Eimeria infection and age group (P < 0.0001, OR = 6), although no statistically si...
Incidence of Eimeria spp. in Fat-Tailed Sheep Breed in Malang, Indonesia
Jurnal Medik Veteriner
Coccidiosis caused by Eimeria spp. is a parasitic disease that affects various animal species, including sheep. This study aimed to detect Eimeria spp. and the degree of infection in the fat-tailed sheep breed in Malang. This study used fecal samples from 62 fat-tailed sheep. Detection of Eimeria spp. was performed by using the flotation method and then observed the morphology of the oocysts found in the fecal samples of fat-tailed sheep. The positive sample was followed by the McMaster test to calculate oocysts per gram (OPG). The results showed that 20 (32%) samples were positively infected by Eimeria spp. with the degree of infection in the severe category.
Prevalence and risk factors of Eimeria spp. natural infection in sheep from northern Paraná, Brazil
Revista Brasileira de Parasitologia Veterinária, 2022
The present study aimed to perform an epidemiological and morphological identification of Eimeria infection in sheep in Brazil. Fecal samples from sheep were collected from 20 farms in northern Paraná, Brazil. An epidemiological questionnaire was used to evaluate the risk factors. Fecal samples containing oocysts per gram of feces (OoPG) ≥1000 were subjected to the modified Willis-Mollay method to perform oocyst identification. Sporulated oocysts were observed microscopically for morphological identification. A total of 807 fecal samples were collected. Based on the morphological characteristics of the sporulated oocysts, 10 species of Eimeria were identified, with main species observed: Eimeira ovinoidalis (98.1%), Eimeria crandallis (87.6%), Eimeria parva (79.1%), and Eimeria bakuensis (60.8%). Only 2.6% (7/268) of the sheep were infected with a single species, 4.8% (13/268) contained two different species, and 92.5% (248/268) were infected with three or more species. The analysis...
Prevalence of Eimeria species in sheep in Eskişehir province
Eurasian Journal of Veterinary Sciences, 2020
Öz Amaç: Çalışma koyun yetiştiriciliğinin yaygın olarak yapıldığı Eskişehir ilinde, koyunlarda bulunan Eimeria türlerinin yaygınlığını araştırmak amacıyla yapılmıştır. Gereç ve Yöntem: Araştırma Eskişehir iline bağlı Seyitgazi, İnönü, Mihalgazi ile Mahmudiye ilçelerinde yetiştirilen 300 merinos ırkı koyun üzerinde yürütülmüştür. Her bir hayvanın rektumundan tekniğine uygun olarak alınan dışkı numuneleri Fulleborn'un doymuş tuzlu su metodu ile incelenmiştir. Pozitif çıkan dışkı numunelerinde gram dışkıdaki Eimeria ookist miktarı (OPG) Modifiye McMaster yöntemi ile belirlenmiştir. Daha sonra, her bir pozitif örnek ayrı petri kutularında üzerine %2,5'luk Potasyum dikromat ilave edildikten sonra laboratuvarda sporlanmaya bırakılmıştır. Sporlanmış ookistlerin ölçümü Nikon Eclipse 80i trinoküler araştırma mikroskobunun x100'lük büyütmesinde DS-5M-L1 dijital kamera sistemi ile mikrometrik olarak yapıldıktan sonra tür teşhisleri ilgili kaynaklardan yararlanılarak yapılmıştır. Araştırmada elde edilen verilerin istatistiksel değerlendirmesinde Ki-Kare (²) testi kullanılmıştır. Bulgular: Araştırma sonucunda muayene edilen 300 adet dışkının 61 (%20 .33)'inde Eimeria ookistlerine rastlanmıştır. Eimeria ovinoidalis (%57,38), E. ahsata (%45,90), E. bakuensis (%42.62), E. parva (%42.62), E. crandallis (%11.48), E. granulosa (%4.92), E. faurei (%3.28), E. intricata (%3.28) ve E. weybridgensis (%3.28) olmak üzere dokuz Eimeria türü teşhis edilmiştir. Enfeksiyon oranı genç hayvanlarda, yaşlı hayvanlardan daha yüksek bulunmuştur (p<0.05). Enfekte hayvanlarda 2-5 türle enfeksiyon yaygın olarak gözlenmiştir. Muayene edilen hayvanlarda klinik bulgulara rastlanmamıştır. Öneri: Bu çalışmada, patojen türlerin yaygın olarak bulunması Koksidiosis'in Eskişehir ili koyunlarında risk oluşturabileceği, hastalıktan korunma ve kontrol yöntemleri konusunda yetiştiricilerin bilinçlendirilmesi ile hastalığın neden olacağı ekonomik kayıpların azaltılabileceği kanaatine varılmıştır.
Detection and Identification of Eimeria species in Naturally Infected Calves at Assiut Governorate
Zagazig Veterinary Journal, 2018
The current study was carried out to investigate the prevalence of Eimeria species among 75 diarrheic calves in Assiut Governorate. Oocysts of Eimeria spp. were found in 46.7% (35/75) of the examined fecal samples from diarrheic calves using light microscope and 88% (22/25) by using PCR. Very high significant difference of Eimeria infection was recorded in calves 3-6 months of age, 73.3% (22/30) and 1 week-3 months of age 28.9% (13/45). The prevalence of Eimeria spp. in calves with diarrhea showed the highest rate in summer (69.2%), followed by winter (36.4%), autumn (25%) and spring (7.7%). Eight species of Eimeria were isolated by parasitological examination. The prevalence of Eimeria spp. was E. zuernii (51.4%) followed by E. bovis (31.4%), E. alabamensis (31.4%), E. cylindrica (25.7%), E. subspherica (14.3%), E. canadensis (11.4%), E. ellipsoidalis (5.7%) and E. auburnensis (2.9%). Single infection of Eimeria spp. was found in 48.6% of the infected calves, whereas mixed infection involved two, three or four Eimeria spp. was observed in 51.4% of the infected calves. In conclusion, season and age of the calves were the most significant aspects connected with the possibility of infection with coccidiosis The PCR is a more reliable, sensitive and less time-consuming approach for diagnosis of Eimeria.
Eimeria Infection in Sheep and Dairy Cattle in
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A cross sectional study was conducted from November 2016 to May 2017 in Akaki Kality subcity of Addis Ababa, central Ethiopia, to determine prevalence and associated risk factors of Eimeria infection in cattle and sheep. A total of 384 randomly selected cattle and sheep fecal samples were collected and examined by centrifugal flotation technique using Sheather's sugar solution to detect the oocysts of Eimeria. Accordingly, the overall prevalence was 57.3% with the prevalence of 55.0 % in cattle and 59.8% in sheep showing relatively higher prevalence in ovine than in bovine. However, there was no significant difference (P>0.05) in the occurrence of infection between the two animal species; neither of sex and body condition of study animals showed the significance. Significant difference (P= 0.0007 in cattle and P = 0.005 in sheep) was observed between age groups with high prevalence in young animals than in adults. There was also strong significant difference (P<0.001 in bo...
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Coccidiosis is of great economic importance in many farm animals. This study involved analysis of 280 faecal samples collected from 12 traditional goat farms from Nay Pyi Taw area, Myanmar. Faecal samples were examined by the flotation method and concentrated oocysts were identified on the basis of morphological characters. Of 280 faecal samples examined, 168 (60.0%) were positive for Eimeria oocysts. Three different Eimeria species were identified and their positive detection rates in the herd were: E. arloingi (25.4%), followed by E. hirci (20.7%) and E. christenseni (13.9%). Identifications were confirmed by 18S rDNA and COI sequences. 18S rDNA sequences showed 100% homology with, respectively, E. christenseni reported from Australia, E. arloingi reported from Australia and Iran, and E. hirci from Australia. COI sequences of E. christenseni, E. hirci, and E. arloingi, respectively, exhibited 98.9%, 98.4%, and 98.5% similarities with those reported from Australia. This is the firs...