Bioeffect of Semi-Purifies Unsabonifiable Components of Rice Bran Oil on Plasma Lipid Levels in Moderately Hypercholesterolemic Rats (original) (raw)
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Studies on Hypocholesterolemic Activity of Stabilized Rice Bran Oil
Journal of Food and Dairy Sciences
The present investigation was carried out to study the effect of stabilized rice bran oil (SRBO) on hypocholesterolemic activity of experimental rats, and also the effect of feeding with stabilization rice bran oil (SRBO) and blend oil on the growth and lipid parameters of serum and liver of rats. Fatty acids composition and unsaponifiable matter of stabilization rice bran oil and blend oil were determined, Results showed that, stabilization rice bran oil had a higher content of saturated fatty acids (SFA), polyunsaturated fatty acids (PUFA) than that of blend oil. stabilization Rice bran oil contained relatively higher concentration of compesterol, stigmasterol, -sitoisterol, cycloartanol, cycloartenol 24-Methylene cycloartanol and oryzanol compared to the blend oil. The serum total and LDL-cholesterol level of rats that maintained on stabilization rice bran oil diet was significantly lower than those fed on blend oil diet. HDL-cholesterol showed a tendency to be higher. Liver lipids of rats fed on stabilization rice bran oil were also markedly lower than their blend oil fed counterparts. Finally, it can be concluded that, using stabilization rice bran oil had the pronounced effects for lowering cholesterol levels of the blood in experimental rats. The cholesterol lowering ability stabilization of rice bran oil appears to be due to unsaponifiable matter and a higher content of polyunsaturated fatty acids.
Rice Bran’s Effect on Total Cholesterol, HDL, and LDL/HDL Ratio on Hypercholesterolemia Rats
Berkala Kedokteran
Hypercholesterolemia is a metabolism disorder characterized by increased or decreased lipid fraction in plasma. The of this research was to prove the effect of Rice bran to decrease of total Cholesterol rate, ratio of LDL / HDL and increase rate of HDL in hypercholesterolemia male white rats (Rattus norvegicus). The Research of the study was pure experiment by using post test randomized control design. Research Sample divided into five groups. The first group as a negative control, the second group as a positive control, and another groups were given rice brand, with various dose: 0,54 ml/a/day, 1,08 ml/a/day, 1,62 ml/a/day. The results of this research is rice brand can decrease of total cholesterol rate (r2=0,964), decreased of ratio of LDL / HDL (r2=0,836), and increase of rate of HDL (r2=0,951) (ANOVA P=0,000). Rice bran can decrease total cholesterol rate, ratio of LDL / HDL and increase rate of HDL blood.
Cholesterol Supplementation Attenuates the Hypocholesterolemic Effect of Rice Bran Oil in Rats
Journal of Nutritional Science and Vitaminology, 2000
Rice bran oil (RBO), when blended with safflower oil (SFO) at the ratio of 7 to 3, has been shown to lower serum cholesterol in humans consuming cholesterol. The mechanism as to how this oil blend exerts its effect is not yet clear. This study examined the effect of cholesterol supplementation on the cholesterol-lowering ability of different RBO/SFO blends. Male Sprague Dawley rats (4wk old) were fed purified diets containing 10% fat with or without the addition of 0.5% cholesterol for 3wk. The fat was either SFO or RBO alone, or the mixture of these two oils at the ratio of 7:3 (7S/3R), 5:5 (5S/5R), or 3:7 (3S/7R). Without cholesterol supplementation, there were no significant differences in the serum and liver total cholesterol levels among different dietary fats. However, the HDL cholesterol level of rats fed the RBO-containing diets (especially in rats fed the 3S/7R diet) was higher than that of rats fed the diet containing SFO alone. This resulted in an increase in the ratio of HDL/total cholesterol-a desirable outcome. Supplementation of the diets with 0.5% cholesterol significantly increased the cholesterol level in both the serum and the liver. Increasing the proportion of RBO in the diet further raised the total cholesterol level in the serum whereas it reduced liver cholesterol. Then, the specific effect of the 3S/7R mix ture on the ratio of HDL/total cholesterol disappeared. These findings suggest that choles terol supplemented at the level of 0.5% in this study masked the cholesterol-lowering effect of RBO. Smaller percentages of polyunsaturated fatty acid (i.e., 18:2n-6) in the RBO-con taining diets than in the SFO diet might have reduced their ability to dispose the circulating serum cholesterol into the liver.
European Journal of Nutrition, 2005
■ Summary Background The cholesterol lowering properties of rice bran oil (RBO) containing differing amounts of non-saponifiable components have not been studied in humans, to our knowledge. Aim of the study To evaluate cholesterol lowering effects of RBO, with low and high amounts of γ-oryzanol (ferulated plant sterols) in mildly hypercholesterolemic men. Methods Mildly hypercholesterolemic men, 38-64 y, starting cholesterol 4.9-8.4 mmol/l (n = 30), consumed 50 g/d peanut oil (PNO) in vehicles for 2 wks during a run-in period, then, without wash-out, were randomly equilibrated (based on initial level of cholesterol) into two groups to consume 50 g/d RBO low (0.05 g/d) or high (0.8 g/d) γoryzanol for 4 wks, in a randomized, controlled, parallel design study. Subjects were free-living and consumed habitual diets with some restrictions. Plasma concentrations of total, LDL-, HDL-cholesterol and triacylglycerol were measured at base line and after 2, 4, and 6 wks. Results The two RBO types were not significantly different with respect to effects on various choles-terol parameters, at 2 and 4 wks, including total cholesterol, LDL-, HDL-and LDL/HDL cholesterol ratio. Low and high γ-oryzanolcontaining RBO feeding for 4 wks lowered total plasma cholesterol (6.3 %), LDL-C (10.5 %) and the LDL-C/HDL-C ratio (18.9 %). Conclusions RBO supplementation at ca. 50 % total fat intake improved lipoprotein pattern in mildly hypercholesterolemic men. Methylated sterols in γ-oryzanol are thought to be largely ineffective at inhibiting dietary cholesterol absorption, but could enhance cholesterol-lowering ability of 4desmethylsterols. Assuming all ferulated sterols become de-ferulated in the gut, low and high γ-oryzanolcontaining RBOs provided intestinal loads of 453 and 740 mg/d free 4-desmethylsterols, respectively. This intestinal load of 453-740 mg/ d of efficacious free plant sterol equivalents had identical effects on lipoproteins. ■ Key words cardiovascular Similar cholesterol-lowering properties of rice bran oil, with varied γ-oryzanol, in mildly hypercholesterolemic men 1 Abbreviations ANOVA analysis of variance BMI body mass index C c h o l e s t e r o l D d a y HDL high density lipoprotein HPLC high performance liquid chromatography EJN 508
Hypolipidaemic effect of rice bran oil and olive oil in hypercholesterolemic rats
2017
Introduction: Hyperlipidaemia is a multifactorial metabolic disorder responsible for the development of cardiovascular diseases. Studies have demonstrated beneficial effect of rice bran oil (RBO) and olive oil in lowering LDL, VLDL and total cholesterol. The study was aimed at evaluating the hypolipidaemic effects of RBO and olive oil and comparing that with a standard hypolipidaemic drug, atorvastatin. Materials and Methods: 30 healthy adult wistar rats of either sex were divided into 5(five) groups consisting of 6 animals each. Atherogenic diet was administered to all animals (except group A) for a period of 8 weeks along with atorvastatin (group C), RBO (group D) and olive oil (group E). Group B was the disease control and received atherogenic diet only. Serum lipid profile and histopathological examination of the abdominal aorta were performed. Results: RBO and olive oil demonstrated comparable efficacy in reduction of LDL, VLDL and total cholesterol. The elevations in HDL level...
The Journal of Nutritional Biochemistry, 2005
Physically refined rice bran oil containing 2-4% nontriglyceride components as compared to other vegetable oils appears to be associated with lipid lowering and antiinflammatory properties in several rodent, primate and human models. These experiments were designed to investigate possible mechanisms for the hypocholesterolemic effect of the physically refined rice bran oil and to examine its effect on aortic fatty streak formation. In the first experiment, 30 hamsters were fed, for 8 weeks, chow-based diets plus 0.03% added cholesterol and 5% (wt/wt) coconut, canola, or physically refined rice bran oil (COCO, CANOLA or PRBO animal groups, respectively). Both plasma total cholesterol (TC) and low-density lipoprotein cholesterol (LDL-C) were significantly reduced in PRBO but not in CANOLA relative to COCO. PRBO also showed a significant 15-17% reduction in cholesterol absorption and significant 30% increase in neutral sterol (NS) excretion with no effect on bile acid (BA) excretion. Both CANOLA and PRBO showed a significant 300-500% increase in intestinal 3-hydroxy-3-methylglutaryl coenzyme A (HMG-CoA) reductase and significant (N 25%) decrease in hepatic HMG-CoA reductase activities with respect to COCO. In a second experiment, 36 hamsters were fed chow-based diets with 0.05% added cholesterol, 10% coconut oil and 4% additional COCO, CANOLA or PRBO. Relative to COCO and CANOLA, plasma TC and LDL-C were significantly reduced in PRBO. Early atherosclerosis (fatty streak formation) was significantly reduced (48%) only in PRBO, relative to the other two. These results suggest that the lipid lowering found in PRBO is associated with decreased cholesterol absorption, but not hepatic cholesterol synthesis, and that the decrease in fatty streak formation with this oil may be associated with its nontriglyceride components not present in the other two diets.
Rice Bran Oil: A Versatile Source for Edible and Industrial Applications
Journal of Oleo Science
Introduction Dietary fat is a crucial factor in the regulation of plasma cholesterol level 1. Dietary fat consumption is correlated with Coronary Heart Diseases CHD and stroke mortality. A diet rich in food of plant origin can significantly retard the development of CHD 2. The vegetable oils rich in polyunsaturated fatty acid, mainly linoleic acid 18:2 , supports hypocholestrolemic effect 1. Rice bran, a co-product of milled rice, and its oil have cardiovascular health benefits. Human consumption of rice bran has been limited, primarily because of the rapid onset of rancidity in rice bran, but methods to stabilize rice bran and to extract its oil have been developed. Interest in rice bran grew from the determination that the inclusion of oat bran in the diet lowers the serum cholesterols 3. Rice Bran Oil RBO typically contains 22 of saturated fatty acid SFA , 43 of monounsaturated fatty acid MUFA and 35 polyunsaturated fatty acid PUFA. This composition is extremely close to the American Heart Association AHA and World Health Organization WHO recommendations on edible oils 6. Research showed the deleterious effects of SFA on blood total cholesterol concentrations, and the fact that RBO lowers blood cholesterol is contrary to these findings. Now research suggests
2012
Diacylglycerol (DAG) oil is becoming quite well known for its beneficial effects on obesity and weight related disorders as they are digested and metabolized in a different way, which significantly reduces body weight. The present study was conducted to evaluate the nutritional effect of rice bran oil (RBO) and DAG-rich rice bran oil on rat model. DAG-rich RBO (46.10% DAG) was prepared in the laboratory by enzymatic (TLIM) glycerolysis process. For the feeding experiment, rats (average body weight 130 g) were divided into two groups, each consisting of eight rats. Group RBO was taken as control while DAG-rich RBO (Group DGR) was taken as experimental. Food efficiency ratio, lipid profile of plasma, liver, mesentery and erythrocyte membrane (EM) lipid profile, plasma leptin content, HMG-CoA reductase activity were measured using standard procedures. The results indicated that DAG-rich rice bran oil significantly decreased body weight of rats and content of total cholesterol and triglyceride in plasma compared to the control RBO. The long term use of DAG-rich rice bran oil might be useful for prevention of obesity and incidence of hypercholesterolemia.