A wide range pH optical sensor with mixture of Neutral Red and Thionin immobilized on an agarose film coated glass slide (original) (raw)

Preparation of a novel optical sensor for low pH values using agarose membranes as support

Sensors and Actuators B: Chemical, 2006

A simple and effective method for the preparation of a new generation of optical sensors based on optically transparent agarose membranes was developed. An optical pH sensor for low pH values was accordingly fabricated by immobilization of Congo Red on epoxy-activated agarose. In an optimized coupling pH of 10 and a dye concentration of 10 −5 mol L −1 , a sensitive sensor with long term stability was obtained.

Agarose film coated glass slides for preparation of pH optical sensors

Sensors and Actuators B: Chemical, 2007

A method for preparation of optical sensors was developed by agarose film coating on aminosilanated glass slides. An optical pH sensor was accordingly prepared by epoxy activation of the agarose film, followed by chemical immobilization of Neutral Red dye. In an optimized coupling pH of 12 and a dye concentration of 10 −2 mol L −1 a pH sensor for a pH range of 2-8.5 was obtained. A theoretical equation was derived using the extended Henderson-Hasselbalch equation that reproduced the measured data well.

Optical sensors for a wide pH range based on azo dyes immobilized on a novel support

Analytica Chimica Acta, 1994

New reactive azo dyes are described which are useful for optical determination of pH. The dyes are covalently linked (via ethylsulfonyl groups) to a novel type of transparent solid films consisting of a thin film of cellulose on top of a polyester support. The resulting membranes exhibit distinct color change when changing the pH and, in most cases, are compatible with LED light sources. Various membranes have been developed with pK, values ranging from 0.5 to 11.28, thus practically covering the whole pH range. Because of the stability of the dyes, the covalent bond and the solid support, the new membranes exhibit storage stability over years, and operational lifetimes of weeks, good reproducibility and a fast (30-60 s) response to pH.

Indicator-Impregnated Agarose Films for Colorimetric Measurement of pH

Journal of laboratory automation, 2016

In this article, we report on the development of indicator-impregnated agarose sensor films for colorimetric estimation of the pH of different standard buffer solutions and urine samples. In contrast to the conventional paper-based dipstick method, the presented method employs dried agarose that was infused with a chemical indicator, which when exposed to the test sample results in an appropriate chromogenic outcome. The color change was then quantified by the smart camera system. The divulged instrument has been calibrated with standard buffers and also tested with clinical urine samples. The systematic study was conducted to characterize the performance of the presented technique, such as interplate variations, time variations, volume variations, and shelf life study of agarose sensor films for the commercial viability of such a technique. The employed method is competent enough to bring a change in the paradigm of dipstick method from being qualitative estimation to quantitative,...

Design of acidochromic dyes for facile preparation of pH sensor layers

Analytical and Bioanalytical Chemistry, 2008

Eight new acidochromic dyes have been synthesised that can be used for optically monitoring pH in the range from 3 to 12. Their corresponding pK a values have been both measured and calculated theoretically by means of density functional theory. The synthesis of these new dyes is facile without the need for chromatographic purification. The dyes can be covalently linked to polymers containing hydroxyl functions such as cellulose, polyurethane hydrogel, and hydroxyalkyl methacrylate. The resulting sensor layers exhibit significant colour changes both in the UV and in the visible spectral range.

Fast and long term optical sensors for pH based on sol–gels

Analytica Chimica Acta, 2003

Long lasting and fast response optical sensor for the detection of pH in the range of 6-12 is described. The sensor is fabricated by spin coating silica sol in the presence of phenol red (PR). The sol is in turn obtained by acidic hydrolysis of tetraethoxysilane (TEOS) and phenyltriethoxysilane (Ph-TriEOS). The performance of the sensor depends on the ratio of Ph-TriOES to TEOS. At the optimal composition, the sensor has a response time of less than 20 s, the response is completely reversible and its lifetime is over 12 months.

Recent Advances in Optical, Electrochemical and Field Effect pH Sensors

2021

Although its first definition dates back to more than a century ago, pH and its measurement are still studied for improving the performance of current sensors in everyday analysis. The gold standard is the glass electrode, but its intrinsic fragility and need of frequent calibration are pushing the research field towards alternative sensitive devices and materials. In this review, we describe the most recent optical, electrochemical, and transistor-based sensors to provide an overview on the status of the scientific efforts towards pH sensing.

Optical sensor for pH monitoring using a layer-by-layer deposition technique emphasizing enhanced stability and re-usability

Sensors and Actuators B: Chemical, 2014

Stable and reliable operation of an optical sensor for pH monitoring is important for many industrial applications of these types of devices. The layer-by-layer deposition technique is a simple and versatile method used to deposit a sensitive thin film on such an optical fibre-based device but creating a coating which can often be destroyed in use in highly acid or alkali solutions i.e. with very low or very high pH. It is thus important to create stable and durable sensors to meet the needs of users for operation under these extreme environments. The main aim of this study has been to prepare a number of such sensors and compare the performance of three different stabilization approaches used for the development of an effective wavelength-dependent pH-sensitive optical sensor. Techniques such as employing heat treatment, the deposition of two layers of a PAH/SiO 2 thin film and the deposition of two layers of APTMS/SiO 2 as topping layers have been studied to determine the optimum approach to creating a stable and reliable sensor-one yielding the same value of peak wavelength for a measurement of a known value of pH and to do so repeatibly. An improvement in performance and in shelf-life, stability and re-usability of the sensor has been achieved by the addition of two bilayers of APTMS/SiO 2 (3-Aminopropyl-trimethoxy silane/Silica nanoparticle) in the work carried out and the results of the investigation undertaken are reported.

Optical behaviour of pH detectors based on sol–gel technology

Analytica Chimica Acta, 2005

Acidity is an outstanding parameter that affect the correct preservation of most part of materials engaged in artworks and heritage objects. In spite of the new generation of advanced analytical devices, the conservation/heritage management sector claims for some kind of simple, robust and low-cost device to monitor environmental acidity wherever necessary. The purpose of this paper is to offer a suitable alternative for the estimation of environmental acidity by means of detectors to be used without electrodes, solutions, pH meters, wires or batteries.

A fluorescent colorimetric pH sensor and the influences of matrices on sensing performances

Sensors and Actuators B: Chemical, 2013

A fluorescent colorimetric pH sensor was developed by a polymerization of a monomeric fluorescein based green emitter (SM1) with a monomeric 2-dicyanomethylene-3-cyano-4,5,5-trimethyl-2,5dihydrofuran derived red emitter (SM2) in poly(2-hydroxyethyl methacrylate)-co-polyacrylamide (PHEMA-co-PAM) matrices. Polymerized SM1 (PSM1) in the polymer matrices showed bright emissions at basic conditions and weak emissions at acidic conditions. Polymerized SM2 (PSM2) in the polymer matrices exhibited a vastly different response when compared to PSM1. The emissions of PSM2 are stronger under acidic conditions than those under basic conditions. When SM1 and SM2 were polymerized in the same polymer matrix, a dual emission sensor acting as a ratiometric pH sensor (PSM1,2) was successfully developed. Because the PSM1 and PSM2 exhibited different pH responses and separated emission windows, the changes in the emission colors were clearly observed in their dual color sensor of PSM1,2, which changed emission colors dramatically from green at pH 7 to red at pH 4, which was detected visually and/or by using a color camera under an excitation of 488 nm. In addition to the development of the dual color ratiometric pH sensor, we also studied the effects of different matrix compositions, crosslinkers, and charges on the reporting capabilities of the sensors (sensitivity and pK a ).