Molecular marker based detection of leaf rust resistance gene Lr34 in Indian bread wheat (Triticum aestivum L.) (original) (raw)
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Genetic resistance is the safest, economical and most effective method of protecting the crop. Durable sources of leaf rust resistance often express a partial, growth stage- specific phenotype referred to as adult plant resistance. One such effective and durable leaf rust adult plant resistance gene deployed world over has been Lr34. Because Lr34 works mostly in adult plants and in combination with other rust resistance genes (such as, Lr34/Yr18, Lr46/Yr29, and Yr30), it is difficult to determine its presence in wheat genotypes. Therefore, there is need to look an alternate method of detecting this resistance gene into the germplasm. Consequently upon availability of a diagnostic marker for Lr34, screening for effective allele of the gene was carried out in Indian wheat genotypes. Among 300 elite wheat lines screened, 60 showed presence of the allele. The resulting genotypes were categorized on the basis of wheat agroclimatic zones as well as the centers responsible for developing t...
Journal of General Plant Pathology, 2011
Fifty wheat varieties along with Jupateco-73 and Morocco were studied for the expression of leaf tip necrosis (LTN), a trait linked with the durable rust resistance gene pair Lr34/Yr18. LTN was frequent (i.e., C6) in nine replications of a field experiment over 3 years in 17 genotypes, and the varieties were considered positive for LTN. In molecular analyses of these varieties, having relative severity values up to 78 for yellow rust and 45 for leaf rust, the 150-bp Lr34/Yr18-linked allele was consistently amplified. Expression of LTN in six of nine replications is an appropriate threshold for predicting the presence of Lr34/Yr18 gene pair, and genotypes can be selected using this trait.
Electronic Journal of Biotechnology, 2011
A total of twenty leaf rust resistance genes (Lr genes) were postulated in nine Egyptian wheat cultivars based on infection types (ITs) expressed on the tested cultivars by 72 Puccinia triticina pathotypes compared with the ITs expressed on the monogenic lines. The most carrier genes cultivars were Giza168 and Misr1 each may contain five genes i.e. Gemmeiza11 was the least cultivar carrying genes; it probably carries just two genes i.e. Lr24 and Lr41. The most postulated genes were Lr19 and Lr24, each postulated within five cultivars followed by Lr41 within four cultivars. Five Lr genes, Lr3, Lr9, Lr10, Lr26 and Lr29 each within three cultivars. The lowest postulated genes were Lr2c, Lr21, Lr22b and Lr37 each of them was postulated within only one cultivar. Five Lr genes, Lr9, Lr10, Lr19, Lr24 and Lr26 were identified by PCR-based molecular marker. The Lr9 gene was identified in cultivar Sids12 while, Lr10 was identified in cultivar Misr1. The Lr19 was present in two cultivars, Misr1 and Misr2. The Lr24 and Lr26 were absent in all the screened Egyptian cultivars. The obtained results for Lr9, Lr10, Lr19, Lr24 and Lr26 marker were in agreement with and confirm their identification by gene postulation. Markers for Lr9, Lr10 and Lr19 may be useful in marker-assisted breeding. Our findings showed the usefulness of the molecular marker in identifying leaf rust resistance genes in wheat cultivars, especially when used in conjunction with multipathotypes test at the pre-breeding stage. This approach may help understanding the wheat -P. triticina interaction and provide information to build an effective management program for leaf rust disease. 1 68 1 6 8 G e m m iz a G e m m iz a 9 9 G em m iz a 1 0 G e m m iz a1 0 G em m iz a1 1 G e m m iz a 11 S id s S id s 12 12 S id s S id s 13 1 3 M is r M is r 1 1 M is r M is r 2 2 G em m iz a7 G em m iz a7 1100 1100 bp bp Figure 1. Polymerase chain reaction of Lr9 marker (1100 bp) in certain Egyptian wheat cultivars.
Identification and Molecular Tagging of Leaf Rust Resistance Gene (Lr24) in Wheat
Agricultural Sciences in China, 2011
This research was aimed to develop AFLP markers co-segregated with gene Lr24 and validate the using for marker assisted selection (MAS). An F 2 population developed from the cross between the resistant line TcLr24 and the susceptible line Thatcher was tested for resistance to the Puccinia triticina races BGQQ and SHRT using for genetic analysis and molecular marker. A total of 224 AFLP primer combinations were used to test the resistant and susceptible parents, as well as the resistant bulk and the susceptible bulk. Four AFLP markers, P-AGA/M-CTT 289 bp , P-AGC/M-CAC 188 bp , P-AGC/M-CAC 162 bp , and P-ACG/M-CGC 239 bp , were co-segregated with Lr24. The AFLP fragment from the primer combination P-ACG/M-CGC was cloned, sequenced and converted into a STS marker named as ASTS212. Thatcher backgrounded NILs and 115 varieties were examined by using this STS marker and the marker SCS1302 607 developed by Gupta. 5R615, 5R616, 1R13, and 1R17 were identified and validated to contain gene Lr24. The marker is dominant and may be useful in identification the resistance gene Lr24 in wheat and wheat breeding programs.
Acta Phytopathologica et Entomologica Hungarica, 2003
Leaf rust resistance genes Lr9 and Lr10 were detected in wheat (Triticum aestivum) genotypes by PCR based STS markers. Out of sixty-two elite wheat genotypes, screened for the presence of leaf rust resistance gene Lr10, nineteen genotypes revealed the presence through PCR analysis with the primers specific to Lr10 gene. Validation of the marker for Lr9 gene in the parental lines followed by successful detection of the gene in F 4 lines out of cross HP1633 (Lr9) X HP1776, was also done. Usefulness of molecular markers for the detection of rust resistance genes in different genotypes is discussed.
STS marker based tracking of slow rusting Lr34 gene in Indian wheat genotypes
Bi-allelic STS marker was used to confirm the presence of adult plant durable rust resistance gene Lr34 in advance generation breeding lines. These lines were scored for leaf rust three times at an equal interval and the area under disease progress curve (AUDPC) was calculated. The lower AUDPC values of Lr34 positive lines confirmed their slow rusting nature. In the absence of direct selection method, the breeders are selecting Lr34 gene carrying lines unintentionally as they showed better resistance. Lines possessing Lr34, an 'undefeated gene', should be used in breeding programme in order to have a broad-spectrum durable leaf rust resistance.
Russian Journal of Genetics, 2006
Molecular STS markers J13, Gb, and J09 were used for screening wheat ( Triticum aestivum L.) accessions previously found to possess leaf-rust resistance genes according to test crosses or phytopathological tests. Specific amplification products were detected in all accessions assumed to possess the Lr9 gene, in nine of ten accessions with the conjectured Lr19 gene, and in 13 of 29 accessions with the conjectured Lr24 gene. Application of STS markers to identification of accessions possessing effective leaf-rust resistance genes is discussed.
Zemdirbyste-Agriculture
Among all cereals, common wheat (Triticum aestivum L.) occupies the largest area of crops worldwide. Wheat leaf rust, caused by the pathogen Puccinia recondita f. sp. tritici considerably reduces the yield of wheat. Breeding for resistance combined with selection based on molecular markers may become an effective tool in the struggle against fungal diseases. Lr19 is the gene that carries high resistance to wheat leaf rust. In recent years, we have seen the development of many molecular markers in close neighbourhood of the Lr19 gene, e.g., SCS265, SCS253, GB, Xwmc221, XustSSR2001-7DL, Xgwm37 and Xgwm44. The aim of the study was to investigate the functionality of molecular markers related to the Lr19 gene. The study was based on two reference genotypes with the Lr19 gene ('Agatha' and Lr19) and three lines strongly infested by wheat leaf rust. Seven molecular markers were analysed, but only two of them (GB and Xwmc221) proved to be specific to the gene under study. These markers were used for analysis of 25 wheat genotypes, which were evaluated for leaf rust resistance in field conditions to confirm their usefulness for selection of breeding material. These findings point to the need of continuous search for functional molecular markers giving repeatable and reliable results.
Screening of the Bread Wheat Varieties for the Leaf Rust Resistance Gene Lr34/Yr18/Sr57/Pm38/Bdv1
The Open Agriculture Journal
Background: The allelic composition of the gene Lr34/Yr18/Sr57/Pm38/Bdv1, which is associated with resistance to leaf rust in varieties of bread wheat (Triticum aestivum L.), has been investigated. Methods: Three DNA markers were used to determine the allelic state of the gene Lr34/Yr18/Sr57/Pm38/Bdv1: the co-dominant molecular genetic markers cssfr5 and csLV34 and the microsatellite marker Xgwm295. Results: Among 32 cultivars evaluated for resistance to leaf rust, 4 were highly resistant, 26 were resistant and 2 were moderately susceptible. Using the co-dominant marker cssfr5 based on the detection of the polymorphic state of one of the exons of the gene Lr34/Yr18/Sr57/Pm38/Bdv1, the Lr34(+) allele, which confers resistance to leaf rust, was found in 25% of the studied varieties. The coincidence between the results obtained with the markers cssfr5 and csLV34 was 84.5%. Conclusion: The data of the conducted molecular genetic analysis were supplemented by observations of the resistan...