α-Amylases by strains of Candida albicans and Fusarium sp.: Expression and characterization (original) (raw)

2016

Abstract

Abstract: In this investigation, a defined medium was inoculated with Candida albicans and Fusarium sp. Incubation was at 28oC. The composition of the medium was potassium dihydrogen sulphate, magnesium sulphate, calcium sulphate, hydrated iron sulphate, manganese sulphate, copper sulphate, zinc sulphate, thiamine, biotin, sodium nitrate (nitrogen source) and galactose (carbon source). Proteins which exhibited α-amylase activity were expressed by the isolates within a period of eight days. The enzymes obtained on days of optimum activity were precipitated at 90 % saturation and dialysed with buffer.The results showed that, by the seventh day of inoculation of the medium, optimum α-amylase activity was expressed by Candida albicans. Production of α-amylase by Fusarium sp. was optimum by the sixth day. The α-amylases constituted by the two isolates, partially purified, were stimulated by Na+ but inhibited by HgCl2 and EDTA.

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