Conjugated Linoleic Acid Reduces Phorbol Ester-Induced Prostaglandin F2 Production by Bovine Endometrial Cells (original) (raw)

Recent interest in conjugated linoleic acid (CLA) research stems from the well-documented anticarcinogenic, antiatherogenic, antidiabetic, and antiobesity properties of CLA in animal models. The objective of this study was to examine the effects of 2 CLA isomers (cis-9,trans-11 and trans-10,cis-12) on phorbol 12,13dibutyrate (PDBu)-induced PGF 2α production in cultured bovine endometrial (BEND) cells. Confluent BEND cells were incubated in the absence (control) or presence of 100 M each of linoleic acid, cis-9,trans-11 CLA, or trans-10,cis-12 CLA for 24 h. After incubation, cells were rinsed and then stimulated with PDBu (100 ng/mL) for 6 h. Compared with untreated cells, PDBu stimulated PGF 2α secretion (+25-fold) within 6 h. The increases in PGF 2α secretion were paralleled by significant induction of prostaglandin endoperoxide synthase-2 (PGHS-2) mRNA (+63-fold) and protein (+1.6-fold) expression. In spite of stimulatory effects on PGHS-2 and peroxisome proliferator-activated receptor δ (PPARδ) mRNA responses, CLA greatly decreased PGF 2α production by PDBu-stimulated BEND cells. There was no evidence for PDBu or CLA modulation of PPARδ protein synthesis in cultured BEND cells. Results indicated that CLA modulation of PGF 2α production by BEND cells was not mediated through PGHS-2 or PPARδ gene repression.