Studies on Gene Expressions at the RNA Level Associated with the Senile Lens Changes in Human Lens Cataract (original) (raw)

2015, Donnish Journal of Medicine and Medical Sciences

Senile lens changes in response to various stimuli are initiated by eye lens proteins coded by genes. In this study, we investigated twelve genes expression at the level of RNA, including those associated with age-dependent changes of lens (Sirt1 and Sirt3 genes), heat shock genes family (HSP70, GRP78, Acry AA genes), inflammatory reaction (TNFα and NF-kβ genes), heat production in mitochondria (UCP1 gene) and oxidative reaction (GSR gene). Those associated with apoptotic and cell senescent stimuli (p53, Caspase3, and p27 genes) were also identified. The genes expression was examined using quantitative Real Time PCR (qRT-PCR) and results were confirmed by conventional RT-PCR. Our results showed that the relative changes in gene expression of Sirt1, p53, HSP70 as well as Caspase 3 were low, Sirt3 and Acry AA were very high, and GSR and GRP78 genes were high, while the other five genes were moderate. All genes were normalized with GAPDH expression. From the twelve gene expression profiles, we concluded the three types of stimuli involve in human lens cataract, oxidation, heat, and inflammation, may contribute to the clouding of the senile lens cataract in Indonesia. We finally hypothesized that not only did the proteins were maintained in the cells of senile lens cataract, but also the mRNAs, especially Sirt3, Acry AA, GSR, and GRP78 mRNAs.