In vitro antiproliferative activity of selected medicinal plants from the North-West of Morocco on several cancer cell lines (original) (raw)
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Pharmaceutical Biology, 2014
Context: Despite several pharmacological applications of the medicinal plants in the Traditional Arabic Palestinian Herbal Medicine in Palestine (TAPHM), studies on their antioxidant properties are still scarce. Objective: This work evaluates the antioxidant and antitumor activities of the ethanol extracts from different parts of six plants: [Arum palaestinum Boiss (Araceae), Urtica pilulifera L. (Urticaceae), Coridothymus capitatus (L.) Reichb (Lamiaceae), Majorana syriaca (L.) Rafin. (Lamiaceae), Teucrium creticum L. (Lamiaceae), and Teucrium capitatum L. (Lamiaceae)] used in the TAPHM. Materials and methods: The antioxidant activity was evaluated for the ethanol extracts by DPPH and b-carotene-linoleic acid assays together with total contents of phenols and flavonoids.
World Journal of Pharmaceutical Research, 2014
Oxidative stress has become widely viewed as an underlying condition of most prevalent human aliments including cancer. The present study aims to evaluate the antioxidant potential of three medicinal plants, grown in Sudan, namely, Ambrosia maritima L., Aristolochia bracteolata L. and Lawsonia inermis L., widely held in traditional practice and reputed to cure tumor. Extraction of the active morphological plants' parts was commenced with dichloromethane then 80 % methanol. The antioxidant activity and phytochemical profile were conducted via 2, 2-diphenyl-1-picrylhydrazyl (DPPH) assay and preliminary phytochemical screening, respectively. Lawsonia inermis MeOH extract ranked the top DPPH active extract (% RSA= 86 ± 0.07%). Other methanolic plants' extracts, except A.bracteolata branches, reported a good antioxidant activity (% RSA > 50 %). Preliminary phytochemical screening of the candidate extracts revealed the presence of coumarins, flavonoids, sterols and tannins. Thus, A. maritima, A. bracteolata and L. inermis by virtue of their antioxidant potential may support their ethnomedicinal uses as antitumor agents.
Pharmaceutical Biology, 2014
Context: Despite several pharmacological applications of the medicinal plants in the Traditional Arabic Palestinian Herbal Medicine in Palestine (TAPHM), studies on their antioxidant properties are still scarce. Objective: This work evaluates the antioxidant and antitumor activities of the ethanol extracts from different parts of six plants: [Arum palaestinum Boiss (Araceae), Urtica pilulifera L. (Urticaceae), Coridothymus capitatus (L.) Reichb (Lamiaceae), Majorana syriaca (L.) Rafin. (Lamiaceae), Teucrium creticum L. (Lamiaceae), and Teucrium capitatum L. (Lamiaceae)] used in the TAPHM. Materials and methods: The antioxidant activity was evaluated for the ethanol extracts by DPPH and b-carotene-linoleic acid assays together with total contents of phenols and flavonoids. For the anti-carcinogenic evaluation, the extracts were tested for the ability to inhibit the proliferation of breast cancer cells (MCF-7) using the MTT reduction assay. Results: Among the extracts, the U. pilulifera had the highest amount of total phenolics, possessing the second highest total flavonoids. It also showed a maximum cytotoxic activity (IC 50 ¼ 63 mg/ml), followed by C. capitatus, and A. palaestinum. Otherwise, the extract of T. creticum was demonstrated to be an efficient scavenger of O 2 (IC 50 ¼ 83 mg/ml), followed by M. syriaca, C. capitatus, T. capitatum, A. palaestinum, and U. pilulifera. Discussion and conclusion: The results suggest that the investigated plants have shown varied antioxidant capacities which were strongly correlated with their contents of phenolics. Accordingly, this study proposes that the therapeutic benefit of these plants can be, at least in part, attributed to its potential inhibition of oxidative processes.
International Journal of Morphology, 2024
This study evaluated the phytochemical screening, antioxidant capacity, and in vitro anticancer activities of four plants namely, Gypsophila capillaris, Anabasis lachnantha, Haloxylon salicornicum, and Horwoodia dicksoniae which belong to four different families: Caryophyllaceae, Amaranthaceae, Chenopodiaceae, Brassicaceae, respectively. The total phenolics, anthocyanins, saponins, total antioxidant capacity (TAC), and DPPH assays were determined by spectrophotometer. In vitro anticancer activity was assessed using two human cancer cell lines; hepatocellular carcinoma (HepG-2) and breast adenocarcinoma (MCF-7) to estimate the inhibition concentration 50 % (IC 50). The results showed that H. dicksoniae has the highest concentrations of phenolics and saponins, while H. salicornicum has the highest DPPH. The highest concentration of TAC was found in G. capillaries. Among the tested extracts, G. capillaries and H. salicornicum have the potential activity against MCF-7 and HepG-2 cell lines in vitro. The content of polyphenols in G. capillaries was profiled by high-performance liquid chromatography (HPLC). The highest concentration among the phenolic compounds was chlorogenic (60.8 µg/ml) while the highest concentration among the flavonoid compounds was hesperidin (1444.92 µg/ml). In summary, G. capillaries and H. salicornicum extracts have potent anticancer activity against HepG-2 and MCF-7 cell lines.
In-Vitro Screening of Some Plant Extracts for Their Potential Anticancer Activity
African Journal of Traditional, Complementary and Alternative Medicines
Background: Natural products have been shown to be reliable sources of anticancer medicines although there is still a consistent demand for new therapeutic natural products for cancer treatment with minimal side-effects. Materials and Methods: In this study, six plant extracts (Grevillea robusta; Euphorbia millii; Euphorbia royleana; Aloe grandidentata; Bauhinia corniculate; and Cassia fistula) were screened for the presence of phytochemical metabolites as saponins, tannins, cardiac glycosides, alkaloids, flavonoids, anthraquinones and sterols, using qualitative tests. Antiproliferative screening assay was performed on a panel of three cancer cell-lines (HepG-2, HCT-116 and MCF-7) using MTT assay, and cytotoxicity was determined using WI-38 human fibroblast cell-line. Results: Some plant extracts reduced cellular growth for the selected cancerous cell-lines. For example, E. royleana and A. grandidentata extracts reduced HepG-2 cellular growth with IC50 of 0.42 and 0.53µg/mL, respectively. Moreover, A. grandidentata and C. fistula reduced cellular growth of MCF-7 with IC50 of 0.37 and 0.67 µg/mL, respectively. Conclusion: E. royleana, A. grandidentata and C. fistula showed significant anti-proliferative activity against HepG-2 and MCF-7 cell-lines with non-cytotoxic nature. This suggests their potential role as anticancer agents against these types of cancer. The presence of flavonoids, sterols and anthraquinones may suggest their enhanced anti-proliferative activities. Therefore, this study has shed light on the possible use of these extracts as potential sources of natural products-based therapy for cancer.
Polish Journal of Food and Nutrition Sciences
Weeds thrive in agricultural environments. However, in certain areas of the world, they are consumed by humans as food, and they can represent a source of valuable active ingredients of ethnomedical interest. In this study, three North African parasitic plants of the Orobanchaceae family, Cistanche violacea, Orobanche crenata and Orobanche lavandulacea, normally considered as weeds, were studied to compare their biological activities. The antioxidant activity of hexane, ethyl acetate, acetone, methanol, and water extracts obtained by sequential maceration was estimated by DPPH, ABTS and FRAP assays. The metabolic profi les of aqueous extracts were studied by 1 H NMR spectroscopy. The oils extracted using hexane as solvent were analyzed by GC-FID and HPLC-DAD to characterize the fatty acid profi le. The cytotoxic effect of the oil from the parasitic plants was also studied on B16F10 melanoma cancer cells. All extracts showed antioxidant activity and differences between extracts obtained using different solvents as well as for different plants have been highlighted. The highest total phenolic content was determined in O. crenata acetone extract (3.53±0.20 mmol GAE/g) and in O. lavandulacea methanol extract (2.29±0.02 mmol GAE/g). 1 H NMR spectroscopy revealed the presence of amino acids, organic acids and carbohydrates. The major fatty acids in the oils were 18:2 n-6, 16:0, 18:1 n-9, and 18:3 n-3. O. lavandulacea revealed to be a highly cytotoxic species for B16F10 melanoma cells. These data suggest that, despite the extreme climate conditions and the damage that can result, the studied desert parasitic plants can represent a novel and precious source of metabolites, with promising biological activities.
Characterization, antioxidant, and cytotoxic effects of some Egyptian wild plant extracts
Beni-Suef University Journal of Basic and Applied Sciences
Background Natural products from plants are very safe as compared to synthetic ones, so the aim of this study was to assess the in vitro antioxidant and antitumor activities of the ethanolic extracts of four Egyptian wild plant species (Varthemia candicans, Peganum harmala, Suaeda vermiculata, and Conyza dioscoridis), as well as polyphenols and flavonoid contents with gas chromatography–mass spectrometry (GC-MS). The antioxidant activity of the four plant extracts was assessed using 2,2-diphenyl-1-picrylhydrazyl (DPPH) to determine 50% inhibition of DPPH radical scavenging activity and reducing power by phosphomolybdate assay. In addition, the chemical composition of the four sample extracts was investigated using GC-MS. The total phenolic and flavonoid levels were also determined. Then, the antitumor activity of the plant extracts against HepG2 cells was determined using a 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay. Results The results showed that Vart...
Study On Antioxidant And Antitumor Activities Of Some Herbal Extracts
2011
The potential of antioxidant activities of the plant extract Gynura procumbens, Achyranthes aspera and Polygenum tomentosum were studied by using 1, 1-diphenyl-2-picrylhydrazyl (DPPH) .Antioxidant activity was qualitatively and quantitatively determined. In this analysis , Ascorbic acid (Vitamin C) was used as the standard .The antioxidant activities were observed all three plant extracts and the EC50 values of G procumbens A.aspera and P.tomemtosum were 13.7 μg /ml,14.37 μg /ml and 14.35 μg /ml. Among these plants, G.procumbens is more potent antioxidant activity then others. Antitumor activities were found with A.aspera (s2) extracts in the dose of 100ppm in carrot disks and G.procumbens (s1) and P.tomentosum (s3) in the dose of 1000 ppm. Therefore, these herbal plants are used in traditional medicines.
Screening of North African Medicinal Plant Extracts for Cytotoxic Activity Against Tumor Cell Lines
European Journal of Medicinal Plants, 2013
The aim of this study was to evaluate the in vitro cytotoxic activity and cellular effects of organic extracts and fractions of four plants; Inula viscosa, Ormenis eiriolepis (Asteraceae), Retama monosperma (Fabaceae) and Marrubium vulgare (Lamiaceae), all of them used in Moroccan traditional medicine. Methodology: The four plants were extracted using organic solvents and screened on a panel of human cancer cell lines including cell types from both solid and haematological cancer origin as well as non-transformed murine fibroblasts. Cell viability assays were