Three-dimensional microscopy with phase-shifting digital holography (original) (raw)
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Space-bandwidth conditions for efficient phase-shifting digital holographic microscopy
Journal of the Optical Society of America A, 2008
Microscopy by holographic means is attractive because it permits true three-dimensional (3D) visualization and 3D display of the objects. We investigate the necessary condition on the object size and spatial bandwidth for complete 3D microscopic imaging with phase-shifting digital holography with various common arrangements. The cases for which a Fresnel holographic arrangement is sufficient and those for which object magnification is necessary are defined. Limitations set by digital sensors are analyzed in the Wigner domain. The trade-offs between the various holographic arrangements in terms of conditions on the object size and bandwidth, recording conditions required for complete representation, and complexity are discussed.
Common-path, single-shot phase-shifting digital holographic microscopy using a Ronchi ruling
Applied Physics Letters, 2019
Phase-shifting digital holography is widely considered to be a groundbreaking method to quantitatively investigate the phase distribution of specimens, such as living cells. The main flaws of this method, however, are that the requirement for several sequential phase-shifted holograms eliminates the possibility of single-shot imaging and complex configurations would also increase the temporal noise. The present paper aims to validate a single-shot, common-path, phase-shifting digital holographic microscopy, employing a self-referencing geometry. A Ronchi ruling, located in the Fourier plane of a standard microscopic imaging system, produces multiple replicas of sample information in the image plane. The phase retrieval algorithm is performed by superposition of the sample-free portion of each replica with the object information, and requires at least three adjacent diffraction orders. To evaluate the performance of the proposed method, the phase distribution of silica microspheres a...
Digital Holographic Microscopy
Optik & Photonik, 2007
Digital holographic microscopy provides quantitative phase contrast imaging that is suitable for high resolving investigations on reflective surfaces as well as for marker-free analysis of living cells. Results from engineered surfaces and living cells demonstrate applications of digital holographic microscopy for technical inspection and life cell imaging.
Digital Holographic Microscopy (DHM)
Imaging & Microscopy, 2006
Digital Holographic Microscopes (DHM) enables strictly noninvasive visualisation of unstained transparent and partially reflective specimens, in real time, by providing simultaneously amplitude and phase changes of a light wave transmitted or reflected. They are used for characterisation of samples at the nanometer scale, for quality control on production line, and for dynamical analysis of biological specimen and micro systems. more than 15 reconstructions per second for 512 x 512 pixels holograms with a standard personal computer.
Applied Optics, 1999
A digital holographic technique is implemented in a microscope for three-dimensional imaging reconstruction. The setup is a Mach-Zehnder interferometer that uses an incoherent light source to remove the coherent noise that is inherent in the laser sources. A phase-stepping technique determines the optical phase in the image plane of the microscope. Out-of-focus planes are refocused by digital holographic computations, thus considerably enlarging the depth of investigation without the need to change the optical focus mechanically. The technique can be implemented in transmission for various magnification ratios and can cover a wide range of applications. Performances and limitations of the microscope are theoretically evaluated. Experimental results for a test target are given, and examples of two applications in particle localization and investigation of biological sample are provided.
Off-axis digital holographic camera for quantitative phase microscopy
Biomedical Optics Express, 2014
We propose and experimentally demonstrate a digital holographic camera which can be attached to the camera port of a conventional microscope for obtaining digital holograms in a self-reference configuration, under short coherence illumination and in a single shot. A thick holographic grating filters the beam containing the sample information in two dimensions through diffraction. The filtered beam creates the reference arm of the interferometer. The spatial filtering method, based on the high angular selectivity of the thick grating, reduces the alignment sensitivity to angular displacements compared with pinhole based Fourier filtering. The addition of a thin holographic grating alters the coherence plane tilt introduced by the thick grating so as to create highvisibility interference over the entire field of view. The acquired full-field off-axis holograms are processed to retrieve the amplitude and phase information of the sample. The system produces phase images of cheek cells qualitatively similar to phase images extracted with a standard commercial DHM.