Cytotoxicity Changes of Cisplatin Drug in the Presence of Magnetic Fields (original) (raw)
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International Journal of Radiation Biology, 2019
Purpose: In this study, the effects of different intensities of Static Magnetic Fields (SMFs) (10, 15 and 25 mT) and different concentrations of cisplatin drug were investigated on the viability percent and IC 50 of the A2780 and A2780-CP cell lines at 24, 48 and 96 hours to show useful potential of SMF as a physical agent to enhance the effectiveness of common therapeutic approaches and decrease of drug resistance to cisplatin anticancer drug. Materials and methods: Magnetic field exposure was performed using a locally designed generator. The cell viability percent, IC 50 and cisplatin uptake in treated cells were evaluated by MTT assay and inductively coupled plasma (ICP), respectively. Results: Increasing of concentration and time of cisplatin drug showed a noticeable decrease in viability percent in sensitive and resistant cell lines compare with control group. These decreases were more significant in resistant cells compared with sensitive cells. The obtained IC 50 values for resistant were greater than the values obtained for A2780 cells. ICP analysis demonstrated an increased uptake of cisplatin after treatment for 48 and 96 h relative to untreated groups in both resistant and sensitive cells. Conclusion: Results showed that A2780 cells were more sensitive to cisplatin than A2780-CP. Studies have shown that SMF can increase the effect of cisplatin on cell viability percent and decrease the resistance of A2780-CP cells by producing large, verruca shaped structures at the surface of the cell membrane.
PloS one, 2014
Beneficial or adverse effects of Static Magnetic Fields (SMFs) are a large concern for the scientific community. In particular, the effect of SMF exposure during anticancer therapies still needs to be fully elucidated. Here, we evaluate the effects of SMF at induction levels that cisPt-treated cancer patients experience during the imaging process conducted in Low field (200-500 mT), Open field (300-700 mT) and/or inhomogeneous High field (1.5-3 T) Magnetic Resonance Imaging (MRI) machines. Human adrenergic neuroblastoma SH-SY5Y cells treated with 0.1 µM cisPt (i.e. the lowest concentration capable of inducing apoptosis) were exposed to SMF and their response was studied in vitro. Exposure of 0.1 µM cisPt-treated cells to SMF for 2 h decreased cell viability (30%) and caused overexpression of the apoptosis-related cleaved caspase-3 protein (46%). Furthermore, increase in ROS (Reactive Oxygen Species) production (23%) and reduction in the number of mitochondria vs controls were seen. ...
African Journal of Traditional, Complementary and Alternative Medicines, 2015
Background: There is a trend towards the use of magnetic fields in medicine. Pulsed electromagnetic fields (PEMFs) technology was based upon 20 years of fundamental studies on the electromechanical properties of bone and other connective tissues. More recently, these magnetic fields have been used to treat several health conditions. There remains continuing concern that exposure to electromagnetic devices may cause adverse effects. The aim of the present study was to investigate the cytological effects induced in rats exposed in a patented medical device that uses PEMFs combined with static magnetic fields (SMFs). Material and Methods: Thirty sexually mature 14-week-old male and female Sprague Dawley rats were distributed into three groups: (a) 5 males and 5 females (independently) exposed to PEMFs combined with SMFs, (b) animals treated with SMFs only, and (c) non-exposed animals. Acridine orange fluorescent-staining micronucleus test and male germ cells analysis were performed according to standardized techniques. Results: A lack of evidence for alterations on micronucleus frequency, on polychromatic erythrocytes percentage, and on sperm counts and morphological characteristics of male germ cells were found in mature rats exposed to PEMFs medical device compared to non-exposed animals. Conclusions: This study suggests that the applied magnetic field generated in a therapeutic device did not have any detectable cytotoxic or genotoxic effect in exposed rats. In view of these findings and the contradictory reports in the literature, it is necessary to carry out more research to help clarify the controversy concerning cytogenotoxic risk associated with therapeutic magnetic fields exposures.
Bioelectromagnetics, 2002
The possibility that magnetic ®elds (MF) cause antitumor activity in vivo has been investigated. Two different experiments have been carried out on nude mice bearing a subcutaneous human colon adenocarcinoma (WiDr). In the ®rst experiment, signi®cant increase in survival time (31%) was obtained in mice exposed daily to 70 min modulated MF (static with a superimposition of 50 Hz) having a time average total intensity of 5.5 mT. In the second independent experiment, when mice bearing tumors were exposed to the same treatment for four consecutive weeks, signi®cant inhibition of tumor growth (40%) was reported, together with a decrement in tumor cell mitotic index and proliferative activity. A signi®cant increase in apoptosis was found in tumors of treated animals, together with a reduction in immunoreactive p53 expression. Gross pathology at necroscopy, hematoclinical/hematological and histological examination did not show any adverse or abnormal effects. Since pharmacological rescue of mutant p53 conformation has been recently demonstrated, the authors suggest that MF exposure may obtain a similar effect by acting on redox chemistry connected to metal ions which control p53 folding and its DNA-binding activity. These ®ndings support further investigation aimed at the potential use of magnetic ®elds as anti-cancer agents.
In Vitro Cellular & Developmental Biology - Animal, 2017
In this study, we reported the effects of simultaneous application of static magnetic field (SMF) and cisplatin as an anticancer drug on the oxidative stress in human cervical cancer (HeLa) cell line and normal skin fibroblast cells (Hu02). The cells were exposed to different SMF intensities (7, 10, and 15 mT) for 24 and 48 h. IC 50 concentrations of cisplatin were obtained by MTT assay. The cytotoxic effects of combined treatment were studied by measuring the intracellular reactive oxygen species content using flow cytometric method and estimation of membrane lipid peroxidation by spectrophotometry. Statistical analysis was assessed using one-way repeated measures analysis of variance (ANOVA) followed by Tukey's test. Based on the obtained results, the highest and lowest death rate, respectively, in HeLa and Hu02 cell lines was observed at the intensity of 10 mT. Also, we found that membrane lipid peroxidation in cancer cells is higher than that of normal counterparts. SMF potently sensitized human cervical cancer cells to cisplatin through reactive oxygen species (ROS) accumulation while it had small effects on normal cells. The combination of both treatments for 48 h led to a marked decrease in the viability percentage of HeLa cells by about 89% compared to untreated cells. This study suggests that conjugation of both physical and chemical treatments could increase the oxidative stress in HeLa cell line and among three optional intensities of SMF, the intensity of 10 mT led to the higher damage to cancer cells in lower doses of drug.
Effects of Magnetic Field and Magnetoliposomes on Mice: Cytotoxicity and Genotoxicity Tests
IEEE Transactions on Magnetics, 2000
In the present work, some biological effects of the AC magnetic field exposure after magnetoliposome sample treatment were investigated through micronucleus assay and blood and peritoneum cytometry. Data showed that after 5 min exposure AC magnetic field might induce inflammatory reactions, genotoxicity, and cytotoxicity. We conclude that under our experimental conditions magnetohyperthermia experiments may be safely conducted by 3-min AC magnetic field exposure.
Pharmacological Research, 2003
Previous works showed that exposure to static and extremely low frequency (ELF) magnetic fields (MF) over 3 mT slows down the growth kinetics of human tumors engrafted s.c. in immunodeficient mice, reducing their metastatizing power and prolonging mouse survival. In the experiments reported here, immunocompetent mice bearing murine Lewis Lung carcinomas (LLCs) or B16 melanotic melanomas were exposed to MF and treated respectively with two commonly used anti-cancer drugs: cis-diamminedichloroplatinum (cis-platin) and N,N-bis (2-chloroethyl)tetra-hydro-2H-1,3,2-oxazaphosphorin-2-amine 2-oxide (cyclophosphamide). The experiment endpoint was survival time. The survival time of mice treated with cis-platin (3 mg/kg i.p.) and exposed to MF was significantly (P < 0.01) longer than that of mice treated only with cis-platin or only exposed to MF, superimposing that of mice treated with 10 mg/kg i.p. of the drug, showing that MF act synergically with the pharmacological treatment. On the contrary, when mice treated with cyclophosphamide (50 mg/kg i.p.) were exposed to MF no synergic effects were observed, the survival curve being exactly the same as that of mice treated with the drug alone. No clinical signs or toxicity were seen in any of the mice exposed to MF alone or along with cis-platin or cyclophosphamide treatment, compared to mice given only the two known drugs. A possible explanation for the synergic effect of MF being found in mice treated with cis-platin could be that the platinum ion stimulates radical production and that MF enhance active oxygen production bringing about changes in tumor cell membrane permeability, influencing positively the drug uptake. Alternatively, or in addition to this, it has been demonstrated that the rate of conversion of cis-platin to reactive species able to bind to DNA, is increased by localized production of free radicals by MF.
Effects of static magnetic field on human leukemic cell line HL-60
Bioelectrochemistry, 2002
A number of structures with magnetic moments exists in living organisms that may be oriented by magnetic field. While most experimental efforts belong to the area of effects induced by weak and extremely low-frequency electromagnetic fields, we attempt to give an attention to the biological effects of strong static magnetic fields. The influence of static magnetic field (SMF) on metabolic activity of cells was examined. The metabolic activity retardation is observed in human leukemic cell line HL-60 exposed to 1-T SMF for 72 h. The retardation effect was observed as well as in the presence of the mixture of the antineoplastic drugs 5 fluorouracil, cisplatin, doxorubicin and vincristine.
Investigation of Potential Genotoxic Effects of Magnetic Field Used in Imaging
Meandros Medical and Dental Journal, 2018
Amaç: Yüksek manyetik alan yaygın olarak biyolojik dokular için zararsız kabul edilse de, biyolojik etkileri konusunda fikir birliği yoktur. Bu çalışma, basit ve yaygın olarak kabul gören Allium testini kullanarak biyolojik dokulardaki manyetik alanın muhtemel genetik hasarlarını araştırmayı amaçlamaktadır. Gereç ve Yöntemler: Aynı büyüklükte sağlıklı Allium cepa (soğan) bitkileri 0, 8, 24, 72 saat 0,5 Tesla manyetik alana maruz bırakılan dört grupta incelendi. Maruziyet sonrası Allium testi yapılmış ve her grup için en az 4.000 hücre incelenmiştir. Bu hücrelerde gözlemlenen kromozom anormallikleri analiz edildi ve fotoğraflandı. Bulgular: Manyetik alan uygulaması, mitotik aktiviteyi olumsuz etkiledi. Kromozomal sapmalar, manyetik alana maruz kalma sürelerine oranla arttı. En sık görülen sapmalar C-metafaz, yapışkanlık (stickiness), geri kalmış kromozom (lagging chromosome), anafaz köprüsü (anaphase bridge), mikronükleus (micronucleus), düzensiz anafaz (irregular anaphase) ve kutup sapması (polar deviation) oldu. Grup Objective: Although the high magnetic field is commonly accepted as harmless for biological tissues, there is no consensus about its biological effects. This study aims to investigate probable genetic damages of magnetic field on biological tissues using a simple and widely accepted method, Allium test. MaterialsandMethods:The same sized healthy Allium cepa (onion) plants were exposed to 0.5 Tesla magnetic field for 0, 8, 24 and 72 hours as groups of four. Allium test was applied and at least 4.000 cells were counted for each group. Observed chromosomal aberrations were analyzed and photographed. Results: Magnetic field application adversely affected the mitotic activity in the experiment group compared to the control. The chromosomal aberrations increased in proportion to increased magnetic field exposure times. The most encountered aberrations were C-metaphase, stickiness, lagging chromosome, anaphase bridge, micronucleus, irregular anaphase, and polar deviation. The group comparisons showed statistically significant differences between the control group and 8, 24 and 72 hour magnetic field exposure groups. Conclusion: This study has shown potential genotoxic and mutagenic effects of high magnetic field on Allium cepa root tip cells using Allium test. Although there is a need for more studies, the data in the study show that the strong magnetic field leads to chromosomal disorders.
Anticancer Effects of Moderate Static Magnetic Field on Cancer Cells In Vitro
Research in Molecular Medicine, 2019
Background: Expansion of the use of magnetic fields in metals, mining, transport, research, and medicine industries has led to a discussion about the effects of magnetic fields and whether their strength is negligible. The aim of this study was to investigate the effects of magnetic field on the viability and proliferation rate of HeLa cells. Materials and Methods: We studied the effects of magnetic field on the viability, proliferation rate and membrane lipid peroxidation of cells, thus, HeLa cells (cancer cells) and human fibroblast cells (normal cells) were used. Initially, the cells were cultured in DMEM and to determine the impact of the magnetic field, the cells were treated with magnetic field at 4 specific intensity levels (0, 7, 14 and 21 mT) and 2 exposure times (24 h and 48 h). The viability percentage and inhibition of cell proliferation were calculated by MTT assay and Trypan blue staining, respectively. Results: Lipid peroxidation of the cell membrane was examined by m...