Biodiversity of a red clover collection based on morpho-productive traits (original) (raw)
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VARIABILITY OF RED CLOVER GENOTYPES ON THE BASIS OF MORPHOLOGICAL MARKERS
and Vegetable Crops, Novi Sad, Serbia Radinović I., S. Vasiljević, M. Zorić, G. Branković, T. Živanović, S.Prodanović (2018): Variability of red clover genotypes on the basis of morphological markers.-Genetika, Vol 50, No.3, 895-906. Red clover (Trifolium pratense L.) is an important forage legume of temperate regions dominantly used as a source of animal food. The present research aimed at assessment of genetic diversity based on morphological markers, through the analyses of five morphological markers in a collection of 46 red clover genotypes. These morphological markers were screened according to the UPOV descriptor (2001) in the trial laid out in a randomized complete block design with three replications. The traits analyzed and investigated were: time of flowering, growth habit, density of hairs, leaf color and intensity of white marks. The average value of Shannon's diversity index for five morphological markers amounted 0.711. Homogeneity analysis (HOMALS) of the same five descriptors accounted for 71.2% of the total variation of the standardized data, with the first and second axis explaining 38.4% and 32.8% of the morphological variability, respectively. Based on this analyses all red clover genotypes were grouped into seven homogeneous groups in two-dimensional space, thus providing visualization of genotypes diversity based on their morphological traits. UPGMA cluster analysis of the same morphological markers allowed the description of four groups with genetic
Journal of Agricultural Sciences, Belgrade, 2022
Red clover is an important perennial fodrage legume and a rich source of highly nutritional voluminous forage for livestock feed, which is continuously improved by plant breeding efforts that rely significantly on trait correlation studies. A two-year field trial was conducted at Rimski Sancevi, Novi Sad, Serbia. The aim of this research was to assess correlations of important morpho-agronomic traits and forage quality properties of 46 red clover accessions of diverse origins. The highest Spearman?s correlation coefficient (0.97, p<0.01) was found between the branch number and internode number per stem in the first experimental year. The green mass yield and the dry matter yield in both years had very high (0.95, p<0.01) and high (0.86, p<0.01) correlations, respectively. The stem height and internode number per stem had intermediate correlations in both years (0.68, p<0.01, and 0.50, p<0.01, respectively), and stem height exhibited an intermediate correlation with gr...
Study of red clover wild populations from the territory of Serbia for the purpose of pre-selection
Genetika, 2014
2014): Study of red clover wild populations from the territory of serbia for the purpose of pre-selection. Genetika, Vol 46, No. 2,[471][472][473][474][475][476][477][478][479][480][481][482][483][484] The aim of this paper was to evaluate the agronomic value of red clover (Trifolium pratense L.) wild populations. The analyzed material was gathered on the territory of Serbia and it was estimated which populations could be involved in the breeding program. 17 red clover populations were included in the research. Investigation was carried out at the experimental field of the Institute for forage crops, Kruševac, Serbia. The field trial was performed during three years (2008 -2010) when data about morphological (green mass, dry mater, plant height, number of stems, number of lateral branches, number of internodes, length and width of a middle leaf lamina) and basic chemical parameters (quantity of crude proteins, crude fiber, ash, fat, NFE) were collected. In this paper, results of the research were processed by application of analysis of variance (ANOVA) and multivariation methods. Cluster analysis based on morphological traits resulted in formation of three clusters and the traits with the largest influence on grouping were: green mass, plant height, length and width of a leaf. Principal component method confirmed this separation and resulted in even more clear grouping of populations. Cluster analysis and PCA method were also applied to the traits of dry matter quality, when four clusters were formed, and the traits with the largest influence on grouping were percentages of protein, fat and fiber. Using both set of traits and several types of analyses contribute to grouping of populations which promise most and whose positive traits such as: high green 472 GENETIKA, Vol. 46, No2,471-484, 2014 mass yield, number of stems as well as the percentage of proteins can be combined for the purpose of getting synthetic varieties.
Zemdirbyste-Agriculture, 2013
The main objectives of this study were 1) to estimate variation in yield, forage quality and morphological traits of newly developed red clover (Trifolium pratense L.) breeding populations of different origin, 2) to compare their yield potential with foreign cultivars, 3) to determine the relationship between studied traits and 4) to identify and select the most promising populations for future breeding work. Eight red clover breeding populations of different origin (OPCD-2, RCOP-1,3,4,5,6,7,8 created within the framework of the forage crops breeding program at the Agricultural Institute Osijek, Croatia) and eight foreign cultivars ('Liepsna', 'Kamaniai', 'Nosovskij 5', 'Pallas', 'Astred', 'Redquin', 'Milvus', 'Amos') were evaluated for thirteen morpho-agronomic and forage quality traits. Field experiment was designed as a randomised complete block with three replications over two consecutive years (2008 and 2009) at the Agricultural Institute Osijek, Croatia. The studied breeding populations/cultivars of red clover significantly varied in yields of green mass and dry matter, plant height, number of internodes, density of hairs, width and length of central leaflet, shape of medial leaflet, leaf to stem ratio, content of crude protein and relative feed value. Croatian breeding populations RCOP-3, OPCD-2 and RCOP-1 had significantly higher total yields of green mass (from 98.50 to 111.82 t ha-1) and dry matter (from 22.61 to 25.64 t ha-1) compared to the observed yields of other populations and foreign cultivars of red clover, which indicates their high genetic potential for yield. Besides the high forage yield, those breeding populations achieved favourable values of quality and other analyzed morphological traits, and they are representing valuable germplasm for further selection work and improving of our breeding program and/or application for plant cultivar registration. Positive correlations were found between forage yields and plant height, number of internodes and between width and length of central leaflet, and these properties were positively correlated with each other. Most quality traits were in negative correlation with yield and other observed morpho-agronomic traits.
Characterizing the molecular and morphophysiological diversity of Italian red clover
Euphytica, 2011
Red clover (Trifolium pratense L.) is the third major forage species in Europe, but there is limited information on the biodiversity and the genetic structure of landraces and natural populations which evolved in this region. The objective of this study was producing such information for Italian germplasm on the ground of molecular and morphophysiological diversity. The study included 16 Italian natural populations from a wide range of environments, four landraces representing the four traditional commercial ecotypes, and two varieties. Eight morphophysiological traits were assessed in a replicated trial under field conditions, whereas two AFLPs primer combinations with 140 polymorphic markers were recorded on a random sample of 13 genotypes per population. Ordination and classification results based on morphophysiological data clearly kept track of the type of germplasm (i.e. landrace or natural population) and its geographic origin, unlike results based on molecular markers. Euclidean distances among populations based on morphophysiological traits were not correlated with Nei's genetic distances based on molecular markers according to Mantel's test. Geographical distances among landrace or natural population material was correlated with distances based on morphophysiological traits but not with those based on molecular markers. The average within-population variation estimated via molecular markers was about 2.6-fold higher than that among populations, preventing an acceptable discrimination among most populations. On average, natural populations tended to have within-population variation similar to varieties and somewhat lower than landraces. Our results have implications for collection, conservation, exploitation and registration in a sui generis system of red clover genetic resources.
ENETIC DIVERSITY IN RED CLOVER (Trifolium pratense L.) USING SSR MARKERS
Momirović (2014): Genetic diversity in red clover (Trifolium pratense L.) using SSR markers-Genetika, Vol 46, No. 3, 949-961. Red clover (Trifolium pratense L.) is one of the main forage species from temperate regions and it's originated in Eurasia where wild-type populations are found in the Caucasus Mountains. Considerable variation for this crop has been recorded for few molecular variations. The identification and understanding of molecular genetic diversity in red clover accessions will help in effective genetic conservation along with efficient breeding programs in this crop. This study has shown molecular diversity using microsatellite markers in red clover accessions from around of the world. In this investigation, 40 genotypes have been selected and analyzed using 15 SSR primers. These primer pairs amplified 1146 polymorphic loci among the genotypes screened. The number of fragments amplified by each SSR primer combination varied from 24 for RCS3681 to 109 for RCS1729 with an average value of 80.78 per primer combination. The SSR marker data was further analyzed using cluster algorithms and Principal coordinates analysis (PCoA). The results indicated that the considerable genetic variations were discovered among the analyzed genotypes. The SSR based clustering could identify the putative pedigree types of the present red clover types of diverse origins
Genetic Resources and Crop Evolution, 2000
Although genotypic and phenotypic markers are used to describe genetic diversity, describing patterns of variation attributable to geographic differentiation is complex. We examined concordance between morphologic and RAPD marker classification of 33 wild red clover populations collected from the Caucasus Mountains, Russia and compared how morphologic and RAPD markers differed in their correspondence to collection site attributes. We also examined if wild red clover populations collected from sites located in areas more conducive to gene flow (i.e. adjacent to roads, or drainage systems) had the same concordance between morphologic and RAPD markers as populations collected from sites less conducive to gene flow. We measured 15 morphologic traits in a common garden and carried out a Random Amplified Polymorphic DNA (RAPD) analysis. There was a significant difference among the 33 populations for 14 out of 15 morphological traits. Morphology clustered the populations into classes that corresponded to three climate regimes. Classification schemes generated by morphologic and RAPD data did not coincide. Morphologic data corresponded with site data for populations collected at all sites. RAPD data corresponded to site data for only those populations collected at sites not conducive to gene flow. A population's adaptation to collection site needs to be considered in using neutral markers to effectively discriminating geographic differentiation. We discuss the practical lessons of this study on the effective collection, conservation and use of plant genetic resources.