Origen genético del ovino criollo mexicano (Ovis aries) por el análisis del gen del Citocromo C Oxidasa subunidad I (original) (raw)
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Con el propósito de conocer los orígenes genéticos mitocondriales de las poblaciones de ovino criollo mexicano, se colectaron 106 muestras de sangre de ovinos criollos mexicanos de los estados de Chiapas, Puebla, Morelos, Hidalgo y Veracruz de México; también se obtuvieron muestras de borregos criollos encastados con Suffolk (n=23) y de la raza Pelibuey (n=15) y BlackBelly (n=6). El gen de Citocromo C Oxidasa subunidad I (COX I) se localiza en la mitocondria. Este fragmento tiene un sitio polimórfico para la enzima HinfI en la posición 5562-5566 que permite diferenciar los genotipos de Ovis aries de origen asiático y europeo. Se llevó a cabo un análisis del polimorfismo del largo del fragmento de restricción (PCR-RFLP). Se amplificó un fragmento de 1053 pares de bases del gen COX I y después una digestión con HinfI, se reveló que todas las muestras de ovejas criollas y sus cruzas presentan el patrón del genotipo B del COX 1. Sin embargo, un individuo BlackBelly presentó genotipo A. En consecuencia, las ovejas locales mexicanas (criollas, borrego Chiapas y Pelibuey) presentan genotipo B, que los ubica con un linaje europeo. Hay evidencia de que ovinos descendientes de animales del oeste de Africa tienen mitocondrias de origen asiático.
Técnica Pecuaria en México, 2009
Con el propósito de conocer los orígenes genéticos mitocondriales de las poblaciones de ovino criollo mexicano, se colectaron 106 muestras de sangre de ovinos criollos mexicanos de los estados de Chiapas, Puebla, Morelos, Hidalgo y Veracruz de México; también se obtuvieron muestras de borregos criollos encastados con Suffolk (n=23) y de la raza Pelibuey (n=15) y BlackBelly (n=6). El gen de Citocromo C Oxidasa subunidad I (COX I) se localiza en la mitocondria. Este fragmento tiene un sitio polimórfico para la enzima HinfI en la posición 5562-5566 que permite diferenciar los genotipos de Ovis aries de origen asiático y europeo. Se llevó a cabo un análisis del polimorfismo del largo del fragmento de restricción (PCR-RFLP). Se amplificó un fragmento de 1053 pares de bases del gen COX I y después una digestión con HinfI, se reveló que todas las muestras de ovejas criollas y sus cruzas presentan el patrón del genotipo B del COX 1. Sin embargo, un individuo BlackBelly presentó genotipo A. En consecuencia, las ovejas locales mexicanas (criollas, borrego Chiapas y Pelibuey) presentan genotipo B, que los ubica con un linaje europeo. Hay evidencia de que ovinos descendientes de animales del oeste de Africa tienen mitocondrias de origen asiático.
Mitochondrial DNA Part A, 2017
The Creole sheep in America is supposed to have originated specifically from the Iberian Peninsula and introduced by the Spaniards during the colonization. However, it is not clear their genetic relationship with Iberian breeds. The genetic origin and diversity of the Mexican Creole sheep (MCS) were investigated by mitochondrial DNA control region nucleotide sequences. DNA sequence from 33 MCS samples from three regions of México revealed 21 different haplotypes. Phylogenetic analysis including European and Iberian sheep haplotypes showed that the MCS population belongs to a differentiated and defined genetic lineage. This finding suggests that the MCS populations may be the result of a founder effect originating from a discrete Iberian population. MCS haplotypes were related to haplotypes found in the Churro Trunk and the Entrefino Trunk groups of Iberian breeds, supporting historical reports. In the Mexican genetic branch, there were also haplotypes reported from Lacaune and Awassi sheep breeds. Although it is uncertain whether a particular breed was involved as a founder of the MCS, these populations have a common phylogenetic origin.
Animals, 2020
The genetic origins and diversity of Creole sheep from five regions of Colombia were investigated based on mitochondrial DNA (mtDNA) variations across 89 sequences from five breeds: one wool Creole sheep (CL) and four hair Creole sheep, including Ethiopian (OPCE), Sudan (OPCS), Pelibuey (OPCP) and Wayúu (OPCW). A global comparison was done using 62 haplotypes from Iberian, African, Indian, Caribbean, Mexican, Caucasian and European sheep based on sequences retrieved from GenBank. This study aimed to identify the maternal origin of Colombian Creole sheep and their genetic relationships at a global level. The results showed 31 different haplotypes from Colombian Creole sheep, which can be assigned to maternal lineage B, the most common lineage found in European sheep breeds and the only one found in several Iberian breed (e.g., Churra, Spanish Merino) that most likely participated in the Creole formation. Additional analyses showed that wool and hair sheep retained a broad genetic ide...
African and European mitochondrial haplotypes in South American Creole cattle
Heredity, 2003
South American Creole cattle are direct descendants of the animals brought to the New World by the Spanish and Portuguese during the 16th century. A portion of the mitochondrial D-loop was sequenced in 36 animals from five Creole cattle populations in Argentina and four in Bolivia. Individuals belonging to the potentially ancestral Spanish breed Retinta were also analysed. Sequence comparisons revealed three main groups: two with the characteristics of European breeds and a third showing the transitions representative of the African taurine breeds. The African sequences were found in two populations from Argentina and three populations from Bolivia, whose only connections go back to colonial times. The most probable explanation for the finding is that animals could have been moved from Africa to Spain during the long-lasting Arabian occupation that started in the seventh century, and from the Iberian Peninsula to America eight centuries later. However, since African haplotypes were not found in the Spanish sample, the possibility of cattle transported directly from Africa cannot be disregarded.
Cytochrome b Diversity and Phylogeny of Six Egyptian Sheep Breeds
Muhammad Gamal Khodary, 2018
Aim: Cytochrome b (Cyt-b) regions of mtDNA have received more attention due to their role in the genetic diversity and phylogenetic studies in different livestock. By using Cytochrome b sequencing, we aimed to clarify the genetic affinities and phylogeny of six Egyptian sheep breeds. Methodology: The genomic DNA was extracted and the specific primers were used for conventional PCR amplification of the Cytochrome b region of mtDNA (1134-bp) in sheep. The alignment of sequences was done to identify the sequence variations and polymorphic sites in the amplified fragments. Results: The results showed the presence of 39 polymorphic sites leading to the formation of 29 haplotypes (accession numbers: MG407500-MG407528) with total haplotype diversity 0.814 and nucleotide diversity 0.00359. The lowest genetic distance was observed between Rahmani and Original Research Article Othman et al.; ARRB, 22(4): 1-11, 2018; Article no.ARRB.38879 2 Saidi while the highest distance was observed between Ossimi and Sohagi. The sequences of 111 analyzed samples were aligned with reference sequences of different haplogroups; A, B, C, D and E. The result showed that 86 out of 111 tested animals cluster with haplogroup B (77.48%), whereas 12 tested animals cluster with each of both haplogroups A and C (10.81%) and one animal belongs to haplogroup E (0.90%) with the absence of haplogroup D. Conclusion: Cytochrome b regions of mtDNA have an important role in the genetic diversity and phylogenetic studies in farm animals as well as many other mammalian species.
Advances in Animal and Veterinary Sciences, 2015
The present study describes the use of PCR and PCR-RFLP methods to discriminate between blood samples obtained from domestic camel, buffalo and sheep. The DNA was extracted from 3 blood samples of each species at 3 different ages and subjected to PCR amplification of a mitochondrial cytochrome b (cyt b) gene segment (358 bp) using universal primers. Subsequent cleavage with 4 restriction endonucleases (AluI, HaeIII, HinfI and Taq I) gaverise to a species-specific pattern on an agarose gel. Results of cleavage by AluI and HaeIII were 2 fragments of different sizes in all animals; Hinf I produced 2 fragments in camel and sheep and 3 fragments in buffalo; Taq I produced 2 bands in camel and sheep while no fragmentation was generated for buffalo. The results suggest that the method of PCR-RFLP using these restriction enzymes can reliably identify chosen species. Moreover, PCR-RFLP is a rapid and simple method for identification of animal species.
Journal of Genetic Engineering and Biotechnology
Background The cytochrome b (Cyt b) gene is one of the most studied mitochondrial DNA (mtDNA) genes to determine sheep’s genetic profile. This study aimed to determine the genetic diversity and relationships of several Indonesian local sheep populations on Java Island, Indonesia, based on the mtDNA Cyt b gene sequences. Blood samples were collected from forty-one individual sheep in seven populations of Indonesia local sheep breeds on Java Island (Priangan = 6, Garut = 6, Batur = 7, Wonosobo = 5, Javanese Thin-Tailed/JTT = 7, Javanese Fat-Tailed/JFT = 5, and Sapudi = 5). DNA extraction was performed on blood samples, and the mtDNA Cyt b gene was amplified using specific primers (Alek-CBF: 5'-CAACCCCACCACTTACAA-3' and Alek-CBR: 5'-CCTTGAGTCTTAGGGAGGTT-3'). The polymerase chain reaction (PCR) products were then sequenced, and data were analyzed using the MEGA version 7.0, DNA SP version 6.0, and NTSYS-pc version 2.11 software. Results A total of 1140 bp complete mtDNA ...
Journal of Molecular Evolution, 1998
The complete mitochondrial DNA (mtDNA) molecule of the domestic sheep, Ovis aries, was sequenced, together with part of the mtDNA of a specimen representing the other major O. aries haplotype group. The length of the complete ovine mtDNA presented is 16,616 nucleotides (nt). This length is not absolute, however, due to heteroplasmy caused by the occurrence of different numbers of a 75-nt-long tandem repeat in the control region. The sequence data were included in analyses of intraspecific ovine molecular differences, molecular comparisons with bovine mtDNAs, and phylogenetic analyses based on complete mtDNAs. The comparisons with bovine mtDNAs were based on the central domains of the ovine control regions, representing both major ovine haplotype groups, and the corresponding domains of Bos taurus and B. indicus. The comparisons showed that the difference between the bovids was 1.4 times greater than the intraspecific ovine difference. These findings suggest that the strains of wild sheep from which domestic sheep originated were more closely related than were the B. primigenius subspecies which gave rise to B. indicus and B. taurus cattle. Datings based on complete mtDNAs suggest that the bovine and ovine lineages diverged about 30 million years before present. This dating is considerably earlier than that proposed previously.