Hepatoprotective and in vivo antioxidant activities of ethanolic extract of whole fruit of Lagenaria breviflora (original) (raw)
Related papers
Pharmacognosy Magazine, 2015
Background: Liver diseases are on rise and remain a serious health problem. Olax subscorpioidea and Distemonanthus benthamianus are two plants used in Ivorian traditional medicine in the treatment of many diseases including jaundice and hepatitis. Objective: The present study was carried out to assess the hepatoprotective and in vivo antioxidant potentials of the hydro-ethanolic leaf extracts of these plants in carbon tetrachloride (CCl 4)-induced hepatotoxicity in rats. Materials and Methods: The plant extracts at doses of 25 and 100 mg/kg bw, and silymarin (25 mg/kg bw) were intraperitoneally (i.p.) injected once daily for 7 days to different groups of rats. Hepatotoxicity was induced on the 7 th day in all the group animals except control. Rats were sacrificed on the 8 th day and blood was collected. Serum biochemical parameters and antioxidant activity were measured using standard procedures. Histopathological examinations of liver rats were also performed. Results and Discussion: Hepatotoxicity induced with CCl 4 was well manifested by significant increase in serum activities of GOT, GPT, ALP and GGT, and enhancement of total bilirubin and TBARS levels. On the other hand, the level of total protein, albumin, α 1-globulin significantly decreased, and DPPH-free radical scavenging activity and TAP were lowered. Pretreatment with plant extracts and silymarin prevent the toxic effects of CCl 4 by decreasing serum enzyme activities, total bilirubin and TBARS levels and improving serum TAP and DPPH-free radical scavenging potential. Histopathological observations showed almost normal hepatic cells with a mild degree of inflammation, lesser fatty infiltration and absence of necrosis among the rats treated at 100 mg/kg of extracts of both the plants. Conclusion: The results suggest that the hydro-ethanolic leaf extracts of O. subscorpioidea and D. benthamianus possesses hepatoprotective and in vivo antioxidant activity.
Hepatoprotective and antioxidant activity of standardized herbal extracts
Pharmacognosy Magazine, 2012
Acacia catechu have been shown to possess widespread pharmacological application against multitude of diseases namely cancer, diabetes, liver disorders, and oxidative stress. Objective: We evaluated the hepatoprotective activity of the standardized herbal extracts against tert-butyl hydroperoxide (t-BH) induced toxicity and their mechanism of hepatoprotective action in human hepatocarcinoma cells (HepG2 cell line). Materials and Methods: The hepatoprotective activity was studied by observing the effect of these herbal extracts on t-BH induced reduction in cell viability of HepG2 cells. In addition, the reducing power of the extracts and their ability to scavenge free radicals were evaluated using two antioxidant assay systems: cell free [oxygen radical absorbance capacity (ORAC), 2,2-diphenyl-1-picrylhydrazyl (DPPH), and [2,2'-azino-bis(3ethylbenzothiazoline-6-sulfonicacid)] (ABTS)] and cell based [cellular antioxidant activity (CAA)]. Results and Discussion: The results obtained showed that these extracts possess significant hepatoprotective activity. This may indicate that the plant extracts contain compounds, which can remove toxic metabolites following t-BH induced toxicity. The extracts exhibited significant antioxidant property as evident by the Trolox values and effective scavenging of DPPH and ABTS radicals. The extracts also demonstrated inhibition of AAPH-induced fluorescence in HepG2 cells. These results indicate the ability of the plant extracts to protect the liver cells from chemicalinduced damage, which might be correlated to their radical scavenging potential. Conclusion: This study demonstrates that these extracts have potential hepatoprotective activity which is mainly attributed to the antioxidant potential, which might occur by reduction of lipid peroxidation and cellular damage.
Antioxidant Property of Plant an Indication for Hepatoprotective Activity
2012
Liver, the largest organ in the body is being evolv ed to maintain the body’s internal milieu and also pro tect itself from the challenges it faces during its functioning. Since it is involved in the biochemical conversio ns of various endogenous and exogenously administered/ingested substances, there is a possibility of generation of various highly reactive species of fr ee radicals. However, it has an inbuilt system like tissue glutathione (GSH), etc to scavenge them off . Inspite of this the free radicals generated by some hepatotoxins like CCl4 may overpower the protective mechanism of the live r and cause hepatic damage. Though the modern medicinal system has grown phenomenally, the drug f or treating hepatic disease is still a dream. Hence , people are looking at the traditional systems of me dicine for remedies to hepatic disorders.
Hepatoprotective and Antioxidant Effects of Silybum marianum Plant in Rats
International Journal for Agro Veterinary and Medical Sciences, 2011
Licorice has been used in Chinese folk medicine for the treatment of various disorders. Licorice has the biological capabilities of detoxication, antioxidation, and antiinfection. In this study, we evaluated the antihepatotoxic effect of licorice aqueous extract (LE) on the carbon tetrachloride (CCl 4)-induced liver injury in a rat model. Hepatic damage, as reveled by histology and the increased activities of serum aspartate aminotransferase (AST), alanine aminotransferase (ALT), alkaline phosphatase (ALP) activities, and decreased levels of serum total protein (TP), albumin (Alb) and globulin (G) were induced in rats by an administration of CCl 4 at 3 mL/kg b.w. (1:1 in groundnut oil). Licorice extract significantly inhibited the elevated AST, ALP and ALT activities and the decreased TP, Alb and G levels caused by CCl 4 intoxication. It also enhanced liver super oxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GSH-Px), glutathione reductase (GR), Glutathione S-transferase (GST) activities and glutathione (GSH) level, reduced malondialdehyde (MDA) level. Licorice extract still markedly reverses the increased liver hydroxyproline and serum TNF-α levels induced by CCl 4 intoxication. The data of this study support a chemopreventive potential of licorice extract against liver oxidative injury.
The rhizomes of Ginger (Zingiberofficinale), Turmeric (Curcuma longa), Licorice (Glycyrrhizaglabra), the bark of Cinnamon tree,(Cinnamomumzeylanicum) and the calyces of red Roselle (Hibiscus sabdariffa L.)are herbs used in thishepatoprotective studies. This study evaluates the hepatoprotective activity of water extract mixtures using carbon tetrachloride (CCl4)-induced liver injury in rats.In vitroantioxidant activity of plant water extracts was determined using DPPH. The water extractmixtures wereadministered for 10 days; on the 10thday all rats were challenged with CCl4 except control group animals. Aspartate aminotransferase (AST), alanine aminotransferase (ALT), and albumin levels were determined to prove the hepatoprotectiveeffect.The enzyme activities were significantly increased in CCl4 treated rats. The four water extract mixtures exhibited significant (P<0.05) protective effect against CCl4-induced hepatoxocity and nephrotoxicity by decreasing the levels of serum markers, specially AST and creatinine , respectively.On the other hand, the serum lipid profiles were slightly improved ; HDL-cholesterol significantly (P<0.05) increased in all the water extract mixtures used.
Objective: To evaluate the hepatoprotective activity of aqueous extract of Oxalis debilis Kunth in carbon tetrachloride (CCl 4)-induced hepatotoxicity in Swiss albino mice. Methods: Hepatotoxicity was induced by CCl 4 30% in olive oil (1 ml/kg intraperitoneally). Mice were treated with aqueous extract of O. debilis at doses of 250 and 500 mg/kg body weight orally for 14 days. There were two groups, pre-treatment (once daily for 14 days before CCl 4 intoxication) and post-treatment (2, 6, 24, and 48 hrs after CCl 4 intoxication). The observed effects were compared with a known hepatoprotective agent, silymarin. Results: Pre-treatment and post-treatment groups of aqueous extract of O. debilis significantly reduced elevated serum levels of serum transaminases, alkaline phosphatase, and bilirubin and increased the level of total protein as compared to CCl 4-treated group. The histopathological study also confirms the hepatoprotection. Preliminary qualitative phytochemical analysis of the plant revealed the presence of phenolic compounds, tannins, flavonoids, and saponins. Conclusion: The results of this study suggest that O. debilis can be used as safe, cheap, and alternative preventive and protective drugs against liver injury. The protective effect observed could be attributed to the presence of various phytochemicals which are responsible for the restoration of liver damage.
2014
The present study investigated the antioxidant and hepatoprotective activity of methanolic rhizome extract of Hedychium Spicatum (MEHS) in CCl4-induced hepatotoxicity model in rats. The hepatoprotective and in vivo antioxidant activity of methanolic rhizome extract of Hedychium Spicatum were evaluated against CCl4induced hepatic damage in rats. The MEHS at dose of 100, 200 and 400 mg/kg were administered orally once daily for seven days. Serum enzymatic levels of serum glutamate oxaloacetate transaminase (AST), serum glutamate pyruvate transaminase (ALT), serum alkaline phosphatase (ALP) and total bilirubin were estimated along with estimation of superoxide dismutase (SOD) and malondialdehyde (MDA) levels in liver tissues. Further histopathological examination of the liver sections was carried out to support the induction of hepatotoxicity and hepatoprotective efficacy. The extract revealed significant activities and substantially elevated serum enzymatic levels of AST, ALT, ALP and...
Journal of Herbs, Spices & Medicinal Plants, 2012
The galls on Terminalia chebula Retz. (Family-Combretaceae) is a well-known herbal drug used to treat various diseases in Indian System of Traditional Medicine. The dried galls are widely commerce in the Indian market and substituted to galls of Pistacia integerrima Stew. Ex. Brandis. The present study aims to investigate antioxidant and hepatoprotective activity of hexane (TCH), ethanol (TCE) and water extract (TCW) of the galls on T. chebula by employing photochemiluminescence and spectrophotometric methods. The results showed that TCE and TCW significantly inhibited 2,2-diphenyl-1-picrylhydrazyl, nitric oxide and superoxide radical in dose dependent manner. Trent of phenol content was as: TCH < TCW < TCE. A significant correlation was shown by total phenol content and free radical scavenging activity of all extracts. The hepatoprotective effect was assayed in CCl4-induced cytotoxicity in a liver slice culture model. The results revealed that significant depletion was observed in lactate dehydrogenase, lipid peroxidation, antioxidative enzymes superoxide dismutase, catalase, and Glutathione reductase on administration of the TCE and TCW or ascorbic acid as standard in the CCl4induced cytotoxicity in the liver. TCE and TCW extracts of galls on T. chebula have prevented significant oxidative liver damage.
2016
Commelina nudiflora, Nephrolepis biserrata and Dillenia suffruticosa have been used in folk medicine for protection against different diseases such as jaundice, although their actual efficiency remains uncertain. The current experiment was designed to investigate the bioactive compounds and hepatoprotective potential of the selected plants against carbon tetrachloride (CCI₄)-induced hepatic oxidative damage in rats and their possible mechanism of action. The total phenolic and flavonoid contents, phytochemical constituents and antioxidant activates were determined in the methanolic extracts and methanolic fractions of plant samples. Phytochemical analysis was performed by gas chromatography and mass spectrometry (GCMS). In this in vivo study, Sprague Dawley rats were pre-treated with various doses of the methanolic extracts of the selected plants once daily for 14 days followed by two doses of CCI₄ (1ml/kg b. wt. ). After 24 hours of the last dosage, the rats were sacrificed and hep...
Journal of Taibah University for Science
In folklore medicine, Ceriops decandra is used to treat hepatitis. However, a scientific evaluation has not been performed. Thus, the present study was carried out to identify the hepatoprotective activity of plant parts (leaf, bark, collar, flower and hypocotyls) of C. decandra. In vitro antioxidant studies were carried out with DPPH, HRSA, NO, FRAP and LPO assays. The LD 50 was calculated and in vivo hepatoprotective activity was carried out with the leaf extract, which was found to be the most potent. The in vivo hepatoprotective activity was performed as follows: Group 1, control animals; Group 2, carbon tetrachloride (CCl 4)-treated animals; Group 3, silymarin (100 mg kg −1 bw p.o.) treated animals; Groups 4, 5 and 6, C. decandra treatment groups (100, 200 and 400 mg kg −1 bw). Histopathological scores were calculated with standard protocols. Of the selected different plant parts, the leaf extract showed maximum antioxidant scavenging properties. A study of the oral acute toxicity found C. decandra extract to be non-toxic up to 2000 mg kg −1 bw. The in vivo hepatoprotective nature of the leaf extract was identified as dose dependent and the levels of SGOT, SGPT, ALP, bilirubin, CHL and LDH were found to be significantly decreased (p < 0.05) compared with hepatotoxin groups. Histopathological scores did not show any significant variations between control and high dose (400 mg kg −1 bw) of leaf extract-treated animals. Preliminary phytochemical analysis of the leaf extract revealed the presence of phenolic groups, alkaloids, triterpenoids, flavonoids, catechin and anthraquinone. In conclusion, the hepatoprotective nature of the C. decandra leaf extract might be due to the occurrence of unique secondary metabolites and their antioxidant scavenging properties.