Molecular Cloning and Analysis of an eIF-4A-related Rat Liver Nuclear Protein. J Biol Chem. 1992 Jun 25;267(18):12928-35. (original) (raw)

In this paper, we report the cloning and analysis of a cDNA encoding a protein M o,f = 47,000 (p47),w hich is localized to the nucleus of rat hepatocytes. identity with a mouse translation initiation factore, IF-4A, which belongs to a family of putative ATP-dependent RNA helicases. We raised polyclonal antibodies against the fusion protein and by indirect immunofluorescence on primary cultures of hepatocytes have demonstrated thpa4t 7 is located in the nucleus. Although on-l2y7 % of hepatocytes showed this nuclear staining, most of the nuclei in proliferating transformed cell lines such as 3T3, PtK-1, and Helaw ere fluorescently labeled. Studieosn serum-starved cells in culture indicated that p47 was expressed in ac ell cycle-dependent manner. Northern analyses demonstrated that the levels of p47 mRNA were high in fetalli ver and droppeds ignificantly after birth to lowle vels in adultl iver. Our data suggest that p47 is developmentally regulated in rat liver at the mRNA level.cytes. The cDNA showed 37% overall sequence