Simplified preparation and characterization of magnetic hydroxyapatite-based nanocomposites (original) (raw)
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Magnetic hydroxyapatite nanocomposites: The advances from synthesis to biomedical applications
Materials & Design, 2021
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Journal of Materials Science
Manganese (II) and iron (III) substituted hydroxyapatite (HA, Ca10(PO4)6(OH)2) nanoparticles were synthesized using wet chemical method. All samples were single-phase, non-stoichiometric and B-type carbonated hydroxyapatite. Compared with pure HA, Mn2+ substituted (MnHA) and Fe3+ doped HA (FeHA) did not demonstrate significant structure deviation. Since ion exchange mechanism was applied for the synthesis process, the morphology and particle size were not significantly affected: all samples were elongated spheroids of around 70 nm. The presence of Fe and Mn was confirmed by energy dispersive X-ray spectroscopy (EDX) while the concentrations were quantified by inductively coupled plasma (ICP). Fe3+ ions were more active than Mn2+ ions in replacing Ca2+ ions in HA lattice structure. The magnetic property of HA was modified by substitution with Fe. The Fe5 (Feadded/Caadded = 5% by molar ratio) was paramagnetic while pure HA was diamagnetic. Results of extraction assay from cells cultured in extracted medium for 72 h suggested that both MnHA and FeHA were non-cytotoxic to osteoblast cells. Meanwhile, the presence of Fe3+ ions in HA demonstrated significant positive effect on osteoblast cells, where the cell number on Fe5 pellets was twice that of pure HA and MnHA samples.
Biology
The development of nanocomposites with tailored physical–chemical properties, such as nanoparticles containing magnetic iron oxides for manipulating cellular events at distance, implies exciting prospects in biomedical applications for bone tissue regeneration. In this context, this study aims to emphasize the occurrence of differential responsiveness in osteoblast-like cells to different nanocomposites with diverse features: dextran-grafted iron oxide (DM) nanoparticles and their hybrid nano-hydroxyapatite (DM/n-HA) counterpart. Here, responsiveness of cells in the presence of DMs or DM/n-HAs was evaluated in terms of cytoskeletal features. We observed that effects triggered by the DM are no more retained when DM is embedded onto the DM/n-HA nanocomposites. Also, analysis of mRNA level variations of the focal adhesion kinase (FAK), P53 and SLC11A2/DMT1 human genes showed that the DM/n-HA-treated cells retain tracts of physiological responsiveness compared to the DM-treated cells. O...
Journal of Materials Science: Materials in Medicine, 2010
Our purpose was obtaining and characterizing a complex composite system with multifunctional role: bone graft material and hyperthermia generator necessary for bone cancer therapy. The designed system was a magnetite enriched collagen/hydroxyapatite composite material, obtained by a co-precipitation method. Due to the applied electromagnetic field the magnetite will induce hyperthermia and cause tumoral cell apoptosis. The complex bone graft system was characterised by XRD, FTIR and SEM, while the hyperthermia was quantify by measuring the temperature increase due to the applied alternative electromagnetical field.
Journal of Nanobiotechnology, 2012
Background: Superparamagnetic nanoparticles (MNPs) have been progressively explored for their potential in biomedical applications and in particular as a contrast agent for diagnostic imaging, for magnetic drug delivery and more recently for tissue engineering applications. Considering the importance of having safe MNPs for such applications, and the essential role of iron in bone remodelling, this study developed and analysed novel biocompatible and bioreabsorbable superparamagnetic nanoparticles, that avoid the use of poorly tolerated magnetite based nanoparticles, for bone tissue engineering applications. Results: MNPs were obtained by doping hydroxyapatite (HA) with Fe ions, by directly substituting Fe 2+ and Fe 3+ into the HA structure yielding superparamagnetic bioactive phase. In the current study, we have investigated the effects of increasing concentrations (2000 μg/ml; 1000 μg/ml; 500 μg/ml; 200 μg/ml) of FeHA MNPs in vitro using Saos-2 human osteoblast-like cells cultured for 1, 3 and 7 days with and without the exposure to a static magnetic field of 320 mT. Results demonstrated not only a comparable osteoblast viability and morphology, but increased in cell proliferation, when compared to a commercially available Ha nanoparticles, even with the highest dose used. Furthermore, FeHA MNPs exposure to the static magnetic field resulted in a significant increase in cell proliferation throughout the experimental period, and higher osteoblast activity. In vivo preliminary results demonstrated good biocompatibility of FeHA superparamagnetic material four weeks after implantation into a critical size lesion of the rabbit condyle.
A simple method of synthesizing nano hydroxyapatite (HA) has been developed by biomimetic wet chemical precipitation method. Powder X-ray diffraction analyses (XRD), Transmission electron Microscopy (TEM) were employed to characterize the formation of HA and its size where the biomolecules Bovine Serum Albumin (BSA), Methionine (MET), and Glycine (GLY) are used as HA growth medium. The results, FTIR showed the interaction between biomolecules-HA is through the C=O (carboxylic) group present in biomolecules and the flurometric studies confirms the stability of the biomolecule at 1000ºC. Such stability of biomolecule has a potential application in biocompatibility, biological response of host by cell attachment & activation, and also provides drug delivery in resorbable bone implants.
Heliyon, 2020
Synthetic hydroxyapatite (HA) due to its high biocompatibility, anti-inflammatory properties, high stability, and a flexible structure in combination with magnetic nanoparticles has the strong potential to be used in modern medicine including tissue engineering, imaging, and drug delivery. Herein, a hydrothermal process was used to prepare magnetite nanoparticles dispersed on the hydroxyapatite nanorods with cetyltrimethylammonium bromide (CTAB) as a surfactant. Characterization study of the synthesized iron oxide-hydroxyapatite (IO-HA) nanocomposite was performed by FT-IR spectroscopy, X-ray powder diffraction, energy dispersive X-Ray analysis (EDX) for elemental mapping, transmission electron microscopy, and vibrating sample magnetometer. Then, the biocompatibility of the synthesized nanocomposite studied by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay and hemocompatibility assay. Focus on this point, curcumin loaded IO-HA (Cur@IO-HA) was developed for exploring the pH-sensitivity of the drug carrier and then evaluating its cellular uptake. The in vitro efficacy of the synthesized nanocomposites as a magnetic resonance imaging (MRI) contrast agent was also investigated. Our results showed that IO-HA nanocomposite is non-cytotoxic and hemocompatible as well as a good pH-sensitive drug carrier and a favorable MRI T2 contrast agent. Comparing to the free curcumin, Cur@IO-HA displayed a good cellular uptake. Taking into account the above issues, IO-HA nanocomposite has the most potential for application as a theranostic MRI contrast agent.
Hydroxyapatite Based Composites for Bone Regeneration
Aim of study: Preparation and characterization of the new nanocomposite, magneto-hydroxyapatite-alendronate (mag-HA-Ald np), for bone tissue scaffolds formation. Material and methods Iron oxide nanoparticles (Fe3O4 np) were added to aqueous solutions of Ca(NO3)2.4H2O and (NH4)2HPO4 for obtaining mag-HA np. The target nanocomposites, mag-HA-Ald np, were synthesized by using aqueous solution of alendronate (Ald), with different ratios. The structure of the prepared nanocomposites were investigated based on spectral (FTIR, XRD, SEM) and EDX analysis. The cytotoxicity of the nanocomposites were performed using MTT assay, to detect the effect of magnetic field application on the cell viability; two culture methods were applied (with and without magnetic field exposure). Results The spectral, FTIR, XRD, SEM as well as EDX measurements confirmed the synthesis of nanocomposite, magneto-hydroxyapatite-alendronate (mag-HA-Ald np) with different ratios. MTT assay results suggested the positive...
Ceramics International, 2020
The mineral constituent of the bone contains non-stoichiometric hydroxyapatite with various substitutional ions. Iron (Fe) and cobalt (Co) are fundamental cofactors, which influence cell respiration and mitosis, respectively. In this study, undoped, Fe 3+-, Co 2+-, and dually-doped HA nanoparticles (NPs) were synthesized by the hydrothermal method. The molar ratio was varied between 0.02 and 0.2. Samples were analyzed using X-ray diffraction (XRD), field-emission scanning electron microscopy (FESEM), vibrating sample magnetometry (VSM), Fourier-transformed infrared spectroscopy (FT-IR), inductively coupled plasma optical emission spectroscopy (ICP-OES), and biological assessment. XRD results confirmed the formation of the hexagonal HA crystal structure. The crystallite size was reduced from 34.6 nm to 4.4 nm. Also, the c-axis dimension and the degree of crystallinity were reduced in all the doped samples. The FESEM results showed a change of morphology from rod-like in the undoped nHA into a sphere-like morphology in the doped-samples. With the addition of ferromagnetic dopant, the magnetic behavior of samples altered from diamagnetic to paramagnetic-like and ferromagnetic behavior. Bioactivity assessment indicated the formation of the HA particle on the surface of all synthesized materials. The antibacterial test demonstrated antibacterial activity against gram-negative (−) E. coli. Moreover, the antibiotic-impregnated samples had a synergistic effect on both gram (−), E. coli, as well as gram (+), S. aureus, bacteria. The MTT evaluation revealed the enhanced cell viability of osteoblast-like cells in the dually-doped NPs. Based on this survey, we can strongly emphasize the potential for bone bioactivity and antibacterial feature of these newly developed samples.
Characterization and influence of hydroxyapatite nanopowders on living cells
Beilstein Journal of Nanotechnology, 2018
Nanomaterials, such as hydroxyapatite nanoparticles show a great promise for medical applications due to their unique properties at the nanoscale. However, there are concerns about the safety of using these materials in biological environments. Despite a great number of published studies of nanoobjects and their aggregates or agglomerates, the impact of their physicochemical properties (such as particle size, surface area, purity, details of structure and degree of agglomeration) on living cells is not yet fully understood. Significant differences in these properties, resulting from different manufacturing methods, are yet another problem to be taken into consideration. The aim of this work was to investigate the correlation between the properties of nanoscale hydroxyapatite from different synthesis methods and biological activity represented by the viability of four cell lines: A549, CHO, BEAS-2B and J774.1 to assess the influence of the nanoparticles on immune, reproductive and re...