Ultraviolet radiation-induced lipid peroxidation in liposomal membrane: Modification by capsaicin (original) (raw)
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Antioxidant effect of inorganic ions on UVC and UVB induced lipid peroxidation
Journal of Inorganic Biochemistry, 1995
Phosphate buffer suspensions of unilamellar liposomes of phosphatidylcholine were irradiated with UVC (254 nm) and UVB (300 nm) light. The irradiation provoked lipid peroxidation and liposome lysis with release of entrapped glucose-6-phosphate. At the same intensity of absorbed light, the photochemical effect at 254 nm is higher than at 300 nm. The addition of copper(II) and manganese(II) reduced both the
Journal of Agricultural and Food Chemistry, 1999
The antioxidant activity of capsaicin, as compared to BHT and melatonin, was determined by the direct measurement of lipid hydroperoxides formed upon linoleic acid autoxidation initiated by AIBN. The formation of four isomeric lipid hydroperoxides was detected after reverse-phase HPLC separation. Data from three detectors, UV absorption, glassy carbon electrode electrochemical detection, and postcolumn chemiluminescence using luminol, were compared. Capsaicin was more effective than melatonin in suppressing the formation of lipid hydroperoxides but not as effective as BHT. The formation of capsaicin and BHT dimers was observed during oxidation, and the dimers were characterized using APCI MS n .
UV-induced change in quercetin antioxidant activity toward benzophenone initiated lipid peroxidation
Journal of the Serbian Chemical Society, 2012
The aim of this work was to estimate the degradation and change in the antioxidant activity of quercetin in the presence of two different mixtures of phospholipids in methanolic solution, under continuous UV-irradiation from three different sub-ranges (UV-A, UV-B and UV-C), in the presence and in the absence of the selected UV-absorbing photosensitizer, benzophenone. Quercetin was employed to control the lipid peroxidation process generated by UVirradiation, by absorbing part of the UV incident light, and/or by scavenging the involved, created free radicals. The results showed that quercetin undergoes irreversible destruction, which was highly dependent on the energy input of the UV-photons, and was more expressed in the presence than in the absence of benzophenone. Simultaneously, quercetin expressed a suppression effect on lipid peroxidation processes in UV-irradiated phospholipid mixtures in both the absence and presence of benzophenone (more or less effective, respectively). In the UV-C-irradiated mixtures, photosensitizing function of benzophenone was significantly reduced due to its strong absorption in the UV-C spectral range, therefore affecting lower antioxidant activity of the remaining quercetin.
Ultraviolet A-Induced Peroxidation of Phosphatidylcholine in Unilamellar Liposomes
Bioscience, Biotechnology, and Biochemistry, 2009
Unilamellar liposomes of 1-palmitoyl-2-linoleoyl-3-snphosphatidylcholine (PLPC) were exposed to ultraviolet A (UVA) light at 37 C. The UVA-induced PLPC peroxidation was inhibited by free radical scavengers, while singlet oxygen quenchers showed slight inhibition. When pre-existing hydroperoxides were reduced, UVA irradiation could not initiate the lipid peroxidation. The UVA-induced reaction of PLPC hydroperoxides with -tocopherol produced addition products. The results indicate that UVA irradiation might dissociate hydroperoxides to alkoxyl and peroxyl radicals which stimulate lipid peroxidation.
HortScience, 1997
Fresh fruit and vegetables are highly perishable because of their active metabolism during the postharvest phase. Previous studies showed that hormic dose of UV cause a delay in the senescence of tomato fruit by about 7 days. The objective of this study was to elucidate whether UV acts on the cell membrane in producing the phenomenon of delayed senescence, since it is known that UV radiation can provoke photooxidation of membrane lipids. Membrane lipid peroxidation was studied in tomato fruit (Lycopersicon esculentum Mill cv. Trust) treated by hormic UV dose, and was followed by assaying products of lipid oxidation during the storage period. We observed the production of lipofuscin-like compounds, malondialdehyde, aldehydes, pentane, ethane, and hydrogen peroxide within few days of the treatment. An increase in the efflux of electrolytes (total, potassium, and calcium) was also observed. An immediate increase in the level of these products of oxidation supports the hypothesis that U...
This study focuses on the behavior of β-carotene and lutein inside multilamellar liposomes under continuous UV-irradiation. The liposomes were obtained by the thin film method and carotenoids (Crts) were incorporated by mixing at various concentrations (0.005, 0.0075, 0.02, 0.07 and 0.5 mol %). Liposomes formation and the presence of Crts inside them were confirmed by SEM microscopy and FT-IR spectroscopy, respectively. The antioxidant/pro-oxidant activity of Crts inside liposomes was determined by the thiobarbituric acid–malondialdehyde (TBA–MDA) test. The investigated Crts acted more or less unexpected (as pro-oxidants) inside the lipid bilayers, interacting with the UV-produced lipid radicals and simultaneously suffering under the UV-irradiation. Their pro-oxidant activity with liposomes and under UV-irradiation could be explained by the formation of unstable adducts in the reaction with peroxyl radicals, or by Crts-cation radicals formation via the electron transfer mechanism. Such tentatively unexpected behavior of carotenoids should be taken into consideration in further carotenoids-based UV-filters projections in cosmetic formulations for skin protection.
Medicinal Chemistry Research, 2018
The aim of the study was to evaluate the effect of Buddleja scordioides Kunth leaves infusion on lipid peroxidation in mice with ultraviolet (UV) light-induced oxidative stress. CD-1 mice were allocated into treatment and control groups (n = 8). Mice in the intervention group were treated with B. scordioides leaves infusion (500 µL equivalent to 0.6534 mg of lyophilized leave infusion/kg of body weight) while the controls received distilled water by gavage administration during 9 days. Both groups were UV irradiated (2.59320175 mW/cm 2) 4 consecutive days: 3 h per day with rest periods of 4 min every hour. B. scordioides leaves infusion had high concentrations of hydroxybenzoic acids, flavonols, hydroxycinnamic acids, and flavone compounds. Mice previously treated with B. scordioides leaves infusion exhibited a significant reduction on malondialdehyde (MDA) concentrations in the liver (77%) but not in kidney and plasma in comparison with the control group. B. scordioides leaves infusion shows hepatoprotective activity via free radical scavenging in mice with UV light-induced oxidative stress.
Action spectrum for UV-induced lipid peroxidation in cultured human skin fibroblasts
Free Radical Biology and Medicine, 1995
Lipid peroxidation was measured by release of thiobarbituric acid-reactive substances (TBARS) into the supematant of cultured human skin fibroblasts. This process is triggered by ultraviolet A (UVA) and ultraviolet B (UVB) radiations. For UVA irradiances and irradiation times up to 40 W • m -2 and 90 min, respectively, the peroxidation response is linear and obeys the reciprocity law. Corresponding values for UVB are 12 W • m 2 and 30 min, respectively. The action spectrum of the peroxidation process shows a continuously increasing response from about 425 to 275 nm. Whereas the UVB to UVA effectiveness ratio lies in the range of l03 to l04 for most in vitro or in vivo UV-induced responses, the ratio is only l0 to 100 for the peroxidation process. Given the solar spectral distribution, solar UVA radiation is by far the most effective in triggering the peroxidation response.