Biochemical Assays of Crude and Purified Oxycarotenoid Extracts Isolated from Coriander Leaves and their Effect on Oxidative Stability of Oils by Rancimat Assay (original) (raw)
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In vivo antioxidant effect of aqueous and etheric coriander (Coriandrum sativum L.) extracts
European Journal of Lipid Science and Technology, 2003
Research has shown that aqueous and etheric coriander extracts, composed of phenolics and carotenoids, exhibit a considerable antioxidant action. Considering the importance of natural antioxidants in the reduction of free radicals and oxidised compounds, coriander extracts obtained through sequential extraction, were administered by gavage to Wistar rats during 30 and 60 d consecutively. At the end of each experimental period, the animals were sacrificed to obtain the blood and the liver. The effect of extracts on plasma and liver lipid peroxidation was assessed by evaluation of thiobarbituric reactive substances. Initially, the aqueous extract demonstrated a superior antioxidant activity in plasma but this was replaced by the etheric extract by the end of the experiment. Of the 2 extracts, in the etheric exerted a superior antioxidant activity in both liver and plasma. The effectiveness of the extracts appears to be related to the biological material that is used (liver or plasma), furthermore to the duration of administration and the type of extract.
Characterization of antioxidant compounds in aqueous coriander extract (Coriandrum sativum L.)
LWT - Food Science and Technology, 2005
An aqueous coriander extract obtained through a sequential extraction process, was analysed using chromatography and mass spectrometry in order to identify the phenolic compounds responsible for its antioxidant activity. Four fractions were identified from the crude extract using chromatography in a silica gel column. Their antioxidant activity, according to the b-carotene/linoleic acid model, was similar to one another but inferior to that of the crude extract and of butylated hydroxytoluene. Of the phenols identified through gas chromatography and mass spectrometry, it was noted that caffeic acid was present in high concentration (4.34 mg/ml in fraction I and 2.64 mg/ml in fraction III), whereas protocatechinic acid and glycitin were present in high concentration in fraction II (6.43 mg/ml) and fraction IV (3.27 mg/ml), respectively. These results, when considered with the recognized antioxidant ability of phenolic acids, suggest that they are principal components responsible for the antioxidant activity of the aqueous coriander extract.
An in vitro and ex vivo Study on Antioxidant Activity of Coriander Seeds
International Journal of Agriculture, Environment and Biotechnology, 2014
Reactive oxygen species (ROS), such as hydroxyl radicals and hydrogen peroxide (H 2 O 2), super oxide anion (O 2 •-), nitrogen derived free radicals are nitric oxide (NO •) and peroxynitrite anion (ONOO •) are fundamental to any biochemical process and represent an essential part of aerobic life and metabolism (Fang et al., 2002). They are constantly generated as normal by-products of mitochondrial respiration and other metabolic activities (Manavalan and Ramasamy, 2001). An imbalance between the generation of ROS and cellular antioxidant 4
Journal of Essential Oil Research, 2011
This study was designed to examine the essential oil composition, phenolic contents and antioxidant activity of the essential oils and methanol extracts from two coriander fruit samples, the first from Tunisia (Tn) and the second from Canada (Can). The highest essential oil yield was observed for Can with 0.44% (w/w) and 0.37% (w/w) for Tn. Forty-five compounds were identified in the essential oils and the main compound of both samples was linalool. The total phenol contents varied between two coriander fruit samples; Can sample presented high polyphenol contents (15.16 mg GAE/g) compared with Tn one (12.10 mg GAE/g). Significant differences were also found in total tannin contents among representing 0.7 mg GAE/g in Can and 0.34 mg GAE/g in Tn. The highest contents of total flavonoids were observed in Can sample with 13.2 mg CE/g. Antioxidants of essential oils and methanolic extracts of these fruit samples were evaluated by using DPPH radical scavenging, b-carotene-linoleic acid bleaching and reducing power activity assays. In all tests, methanolic extracts of coriander fruits showed better antioxidant activity than essential oils. DPPH scavenging ability of methanolic extracts of two fruits was higher than that of synthetic antioxidant BHT (IC 50 = 25 μg/mL). Both extracts and essential oils had lower b-carotene bleaching activity than BHA and BHT and had lower reducing power than ascorbic acid (EC 50 = 40 μg/mL).
Coriander Seed Extract ( Coriandum Sativum L ) as an antioxidant
International Journal of Health and Pharmaceutical (IJHP), 2022
Diabetes mellitus (DM) is a disease characterized by hyperglycemia, lipoprotein abnormalities, increased basal metabolic rate, enzyme deficiency, and high oxidative stress. This research is an experimental study that aims to explore coriander seed extract (Coriandrum sativum L) using Post-Test Only Control Group Design. This study to was conducted to see the antioxidant potential (DPPH, ABTS, LC-MS/MS) of coriander seed extract (Coriandrum sativum L). Based on the test results, coriander seed extract contains very high phytochemicals, one of which is alkaloid compounds, and in vitro test results found DPPH antioxidant with an average IC50 value of 14.33 g/mL and at the lowest concentration (6.25 g/mL). g/mL), CSE also showed the highest antioxidant activity against ABTS trapping with an average IC50 value of 74.07 g/mL and the results of LC-MS/MS obtained samples of coriander extract containing 0.00009% chlorogenic acid and quercetin compounds. of 0.00012%.
This study is designed to examine the phenolic content and composition and antioxidant activity of the methanol extracts of Coriandrum sativum L. seed, whole fruit and pericarp. Seed presented the higher antioxidant capacities with 77.46 mg GAE/g followed by whole fruit (65.58 mg GAE/g) and pericarp (61.41 mg GAE/g). Total phenol contents were 15.55 mg GAE/g in seed, 12.10 mg GAE/g in whole fruit and 2.92 mg GAE/g in pericarp. Concerning total flavonoid contents, they were 13.20 mg CE/g in whole fruit, 19.98 mg CE/g in seed and 1.01 mg CE/g in pericarp. Condensed tannins were present in whole fruit and seed with 0.70 and 0.62 mg CE/g, respectively. Pericarp showed the lower tannin content (0.24 mg CE/g). Among the polyphenols studied, 18 were identified in whole fruit, 15 in seed and 10 in pericarp. The main phenolic compound was dihydroxyphenolic acid with 85.01 mg/100 g in whole fruit, 101.5 mg/100 g in seed and 10.52 mg/100 g in pericarp. Antioxidant activities of the methanolic extract from coriander fruit different parts were evaluated by using DPPH radical scavenging, β-carotene-linoleic acid bleaching and the reducing power tests. All fruit parts extracts showed remarkable DPPH radical scavenging activity with IC50 values of 42 and 32 μg/mL in seed and whole fruit, respectively. However, in pericarp extract DPPH scavenging ability was higher than that of BHT (IC50 = 25 μg/mL). The extracts had lower βcarotene bleaching activity than BHA and BHT. Coriander fruit extracts presented a very low reducing power ability (EC50 ≈ 700 μg/mL) compared to ascorbic acid (EC50 = 40 μg/mL).
Antioxidant Profile of Coriandrum Sativum Methanolic Extract
INTERNATIONAL RESEARCH JOURNAL OF PHARMACY, 2014
Methanolic extract of Coriandrum sativum (coriander) seeds was analyzed for the presence of various antioxidants; ascorbate, riboflavin, tocopherol, polyphenols and in vitro antioxidant potential. The extract, rich in polyphenolic compounds (18.696 ± 0.12 mg/g dry seeds) was subjected to HPLC analysis for identification and quantification of phenolics. Gallic acid (173.656 µg), caffeic acid (80.185 µg), ellagic acid (162.861 µg), quercetin (608.903 µg) and kaempferol (233.70 µg)/g dry seeds were identified. Antioxidant activity of the extract was determined by various mechanisms including DPPH free radical scavenging, metal induced protein and lipid oxidation inhibition and protection of DNA against H2O2 induced damage. Coriander had excellent free radical scavenging activity with IC50 value 0.4 mg dry seed weight, whereas comparatively higher IC50 was observed with metal ion chelating assays (7.2-8.0 mg dry seed weight). The results suggest that polyphenols including gallic acid, caffeic acid, ellagic acid, quercetin and kaempferol are the principle component responsible for high antioxidant activity of methanolic extract of coriander seeds. This is the first report on detailed analysis of antioxidant composition and antioxidant properties of methanolic extract of coriander seeds.
Journal of Food Science and Technology, 2012
Coriandrum sativum L. (coriander), an everyday spice in the Indian kitchen is known to add flavor to the cuisine. It is an annual herb belonging to the Apiaceae (Umbellifera) family. The hydro-alcohol extract of Coriandrum sativum L. at the dose of 1 mg/ml was subjected to a series of in vitro assays viz. 2, 2′-diphenyl-1picrylhydrazyl, lipid peroxidation by thiobarbituric acid, reducing power and nitric oxide (NO) radical scavenging in order to study its antioxidant efficacy in detail. The amount of flavonoids in 70% ethanol extract was found to be 44.5 μg and that of the total phenols was 133.74 μg gallic acid equivalents per mg extract. The extracts of the leaves showed metal chelating power, with IC 50 values, 368.12 μg/ ml where as that of standard EDTA was 26.7 μg/ml. The IC 50 values for 2, 2′-azino-bis (3-ethylbenzothiazoline-6sulphonic acid radical scavenging was 222 μg/ml where as that of standard ascorbic acid was 22.6 μg/ml. The NO scavenging activity of the extract of the leaves showed IC 50 value of 815.6 μg/ml; at the same time the standard BHA had 49.1 μg/ml. All the plant extracts provided DNA damage protection; however, the protection provided at the dose of 8 μg/ml was comparable to that of standard gallic acid. The Coriandrum sativum leaf extract was able to prevent in vitro lipid peroxidation with IC 50 values; 589.6 μg/ml where as that of standard BHA was 16.3 μg/ml. Our results also showed significant ferric reducing power indicating the hydrogen donating ability of the extract. This study indicated the potential of the leaf extract as a source of natural antioxidants or nutraceuticals that could be of use in food industry with potential application to reduce oxidative stress in living system.
Effect of coriander seeds on hexachlorocyclohexane induced lipid peroxidation in rat liver
Nutrition Research, 2001
The effect of prefeeding of coriander seed powder (CSP) at 10% level on hexachlorocyclohexane (HCH) induced oxidative stress in rat liver was studied. The prefeeding of rats with CSP was found to reduce the HCH-induced rise in conjugated dienes, hydroperoxide and malondialdehyde (MDA) contents in liver. The intra peritonial injection of HCH reduced hepatic superoxide dismutase (SOD), catalase, glutathione S-transferase (GS-t), glucose-6-phosphate dehydrogenase (G-6-PDH) and glutathione reductase (GSSGR) activities in liver, where as the hepatic glutathione peroxidase (GSH-Px) and kidney ␥-glutamyltranspeptidase (GGT) activities were elevated. The prefeeding of CSP per se increased hepatic SOD, catalase, GS-t, G-6-PDH and GSSGR activities as well as in CSP prefed and then HCH-administered rats. The work shows the modulation of hepatic antioxidant system as a result of the prefeeding of CSP, which were otherwise reduced by HCH injection. The results reported here elicit the antioxidative effect of coriander seeds against HCH-induced formation of free radicals in rat liver.
Journal of Dietary Supplements, 2010
The effect of coriander seed powder (CSP), a culinary spice, on dimethyl hydrazine (DMH)-induced oxidative stress and toxicity in rats was investigated. Six groups of 6 male rats each were maintained for 12 weeks as (a) Control; (b) DMH (60 mg/kg body weight) injected; (c) 5% CSP incorporated diet; (d) 5% CSP incorporated diet + DMH; (e) 10% CSP incorporated diet; and (f) 10% CSP incorporated diet + DMH. The rats were sacrificed after 12 weeks. The results revealed that DMH administration lead to an increase in hepatic lipid peroxidation associated with reduction in levels of glutathione (GSH), activity of superoxide dismutase (SOD), and catalase and glucose-6-phosphate dehydrogenase. The coadministration of CSP and DMH diminished the hepatic malondialdehyde (MDA) significantly as compared to DMHalone administered rats. The intake of coriander seeds at 10% level also enhanced the hepatic GSH-redox system by elevating GSH-Px, GSSGR, and GST activities. The DMH-induced decline in SOD and catalase activities was brought to normal by 10% CSP. The coadministration of CSP and the DMH produced a significant reduction in MDA and enhancement in catalase activity as compared to control. Coriander powder at 5% and 10% levels produced a significant rise in colonic catalase and GSH-Px. The coriander seeds produced significant beneficial effects by reducing the DMH-induced oxidative stress and enhancing the tissue levels of antioxidant/detoxification agent in tissues.