Gangliosides and Neutral Glycolipids in Guinea Pig Adrenal Glands1 (original) (raw)

The Journal of Biochemistry, 1984

Abstract

Glycolipids were isolated from the adrenal glands of seven strains of guinea pig by DEAE-Sephadex and Iatrobeads column chromatographies. The average lipid-bound sialic acid content of the adrenal glands was estimated to be 96.0 +/- 30.4 nmol/g fresh tissue. The ganglioside fraction contained two major gangliosides which accounted for 82% of the total lipid-bound sialic acid. They were identified as N-acetylneuraminylgalactosylceramide (GM4) and N-acetylneuraminyllactosylceramide (GM3). The neutral glycolipid fraction contained one major and three minor glycolipids. The major glycolipid was identified as galactosylceramide, which accounted for 81% of the total glycolipid. The other three glycolipids were identified as Forssman glycolipid (7%), lactosylceramide (5%), and gangliotriaosylceramide (7%). In all strains examined, the glycolipid patterns were similar.

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