The hypervariable domain of the mitochondrial control region in Atlantic spiny lobsters and its potential as a marker for investigating phylogeographic structuring (original) (raw)
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Marine Biology, 2011
To investigate population differentiation in a comparative and historical context, segments of the mitochondrial cytochrome c oxidase subunit I gene and the control region were sequenced in Panulirus argus from nine sites along approximately 1,500 km of the Northern Caribbean Sea (n = 326) and analyzed with respect to available panulirid data. A mismatch analysis and Fu’s F S test uncovered a signature of historical population expansion around the time of the Last Glacial Maximum. Significant population structure was not detected in the area. The data supported a hypothesis of panmixia resulting from ongoing larval transport by ocean currents and historical population expansion. Despite high intraspecific divergence levels at COI within Panulirus argus and several other Panulirus species, genetic species identification through DNA barcoding was feasible using either a modified distance threshold or a character-based approach.
Mitochondrial DNA panmixia in spiny lobster Palinurus gilchristi suggests a population expansion
Marine Ecology Progress Series, 2005
The spiny lobster Palinurus gilchristi is endemic to the deep shelf waters along the southern coast of South Africa, where it supports a commercial fishery. Mark-recapture studies and phenotypic differences suggest there are 2 populations of this species along the coast, but it is unknown if the observed differences have arisen because of low gene flow and subsequent genetic differentiation. To investigate population structure and the physical processes that may have influenced gene flow, a portion of the mitochondrial DNA control region was sequenced for 187 lobsters across the entire range of the species. An analysis of molecular variance showed no significant genetic difference between the 2 putative populations. A mismatch distribution and Fu's F S test indicated that this species has undergone a fairly recent demographic expansion (population size and geographic range). The genetic structure of this species could be panmictic due to a high amount of gene flow between the 2 regions during the larval stage, when the larvae are carried downstream by the Agulhas Current. Furthermore, the lack of genetic differences between the 2 putative populations could be the result of a recent demographic expansion accompanied by low diversity of haplotypes produced by a leading edge effect from the expansion.
Indonesian Aquaculture Journal
Populations of spiny lobsters worldwide are threatened by overfishing, while its supply from aquaculture is currently insufficient to meet the market demand. This current study investigated the genetic structure of two economically important spiny lobsters, P. homarus and P. ornatus sourced from the Indian Ocean and South China Sea. Fragments of the cytochrome oxidase subunit-I (COI) gene of the mitochondrial DNA of 71 P. homarus and 42 P. ornatus collected from 6 and 5 fish landing sites in Indonesia, respectively, were sequenced. Homologous sequences from the Indian Ocean and South China Sea available at GenBank were included in the analysis. No genetic differences were observed in P. ornatus populations from the two geographic regions (xST = -0.005) while a diminutive difference was found in the populations of P. homarus (xST = -0.002 and 0.009). These results, combined with a negative Tajima’s D estimates, points to a deficit of nucleotide variation relative to the expectations ...
Over the past years fishery managers and scientists have been addressing concerns on the spiny lobster Panulirus argus fisheries, due to unsustainable harvesting throughout the coastline of the Americas. Commercial fishing commonly overexploits stocks, and current landings of P. argus fisheries throughout the western central Atlantic indicate a resource that is being exploited beyond its limits. Knowledge regarding population subdivision is critical to sustainable fishery management and seems to be the correct approach as a problem-solving strategy in P. argus fisheries. The goal of this article is to provide baseline information in order to help researchers and fishery managers build knowledge base that would be used to facilitate the conservation and support the establishment of specific regional management policies for this valuable resource. With the advance of DNA technologies new approaches started to be applied on population genetic studies. Molecular ecologists have begun to use new techniques that allow them to subdivide a particular species into a number of genetically distinct stocks. The application of both microsatellite markers and DNA sequencing to the population genetics of P. argus is believed to be the method of choice in detecting heterogeneity and identifying lobster stocks. However, both genetic and ecological tools (e.g. fully understanding of hydrological patterns along the coast) should be integrated to efficiently manage P. argus fisheries in Brazil and in the Caribbean.
Fisheries Science, 2007
The mitochondrial DNA sequence for the COI region was determined for 73 Panulirus japonicus individuals collected at three sample locations in Japan. Fifty-one haplotypes, including 69 polymorphic sites without insertion and deletion, were detected, of which three dominant haplotypes were shared among the three sample locations. The nucleotide and haplotype diversities at the three sample locations were in the ranges of 0.009-0.010 and 0.959-0.990, respectively. No significant population subdivision was detected among the sample locations based on the Fst value and net nucleotide substitution rate, and analysis of the molecular variance model (AMOVA). There was no characteristic geographic distribution pattern for these haplotypes. These results indicate there is no genetic differentiation between P. japonicus populations in Japan, and support the hypothesis that benthic individuals of P. japonicus are sustained from a population of a common pool of long-period phyllosoma larvae through long-distance larval transport within the Kuroshio Subgyre.
International Journal of Molecular Sciences, 2014
The pronghorn spiny lobster Panulirus penicillatus is a highly valuable species which is widely distributed in Indo-West Pacific and Eastern Pacific regions. Mitochondrial DNA control region sequences (566-571 bp) were determined to investigate the population genetic structure of this species in the Indian Ocean. In total, 236 adult individuals of Panulirus penicillatus were collected from five locations in the Indian Ocean region. Almost all individuals had a unique haplotype. Intrapopulation haplotype (h) and nucleotide (π) diversities were high for each locality, ranging from h = 0.9986-1.0000 and π = 0.031593-0.043441. We observed distinct genetic isolation of population located at the northwestern and southwestern edge of the species range. Gene flow was found within
PloS one, 2011
The pronghorn spiny lobster, Panulirus penicillatus, is a circumtropical species which has the widest global distribution among all the species of spiny lobster, ranging throughout the entire Indo-Pacific region. Partial nucleotide sequences of mitochondrial DNA COI (1,142-1,207 bp) and 16S rDNA (535-546 bp) regions were determined for adult and phyllosoma larval samples collected from the Eastern Pacific (EP)(Galápagos Islands and its adjacent water), Central Pacific (CP)(Hawaii and Tuamotu) and the Western Pacific (WP)(Japan, Indonesia, Fiji, New Caledonia and Australia). Phylogenetic analyses revealed two distinct large clades corresponding to the geographic origin of samples (EP and CP+WP). No haplotype was shared between the two regional samples, and average nucleotide sequence divergence (Kimura's two parameter distance) between EP and CP+WP samples was 3.8±0.5% for COI and 1.0±0.4% for 16S rDNA, both of which were much larger than those within samples. The present result...