Identification of Different DermatophytesIsolated From Cattle, Cats and Horses Suffered From Skin Lesions (original) (raw)
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Morphological detection of dermatophytes isolated from cattle in Wasit province
Iraqi Journal of Veterinary Sciences
This study aimed to diagnose the skin lesions in cattle which were suspected to be ring worm in order to determine the species causing these lesions. A total of 40 samples of skin desquamation and hair lesions were collected from cattle in a farm located in the Wasit province during the period October 2021 to March 2022. Diagnosis used to be carried out the usage of direct microscopy of potassium hydroxide, isolation of the fungus the use of Sabouraud dextrose agar supplemented with chloramphenicol and cycloheximide, as well as by way of detecting those fungi through staining with Lactophenol cotton blue. The diagnosis results revealed that infection of cattle with ringworm represented 20/40 (50%) of skin lesions and the majority of the common dermatophytes spp was identified as Trichophyton mentagrophytes 15/20 (75%) and Microsporum canis5/20 (25%) depending on the fungal growth. In conclusion, the most common skin lesions of cattle in Wasit province were identified as ring worm.
Ringworm in cattle and man caused by Microsporum canis: Transmission from dog
Dermatophytosis ,caused by many dermatophytic fungi,is an important cutaneous mycotic zoonosis of global significance. Microsporum canis, a zoophilic dermatophyte, was identified as the prime cause of ringworm in two cross-bred, female calves which belonged to a private dairy farm in Gujarat, India. The small, discrete, scaly lesions were observed on the skin of the head and neck of the affected young cattle.The direct examination of the skin scrapings under light microscope showed hyaline,branched ,septate hyphae and arthrospores by potassium hydroxide technique. The fungus was isolated in pure culture from the skin scrapings on Sabouraud dextrose agar and dermatophyte test medium (DTM) .The detailed morphological examination of fungal growth in Narayan stain confirmed the identity as Microsporum canis. The retrospective epidemiology indicated that both the calves contracted the infection from a young male pet dog. In addition, one three-year-old female child in the family also acquired ringworm infection from the diseased dog. The skin scrapings from the calves, dog and child when examined under Wood's lamp exhibited a bright greenish yellow fluorescence suggesting Microsporum infection. Microsporum canis was also identified in the skin lesions of pet dog and the child by direct microscopy as well cultural isolation on mycological media. Interestingly, the isolates of Microsporum canis from cattle, dog and man did not reveal any difference in their cultural and morphological characteristics. The findings of this investigation indicated that transmission of Microsporum canis from the dog to cattle and man is possible. As animal dermatophytes are communicable to humans, care must be exercised while handling the sick animal or collecting the skin scrapings or applying drug on the skin lesions. It is emphasized that Narayan stain should be widely used in all public health and microbiology laboratories for studying the detailed morphology of fungi which are incriminated in the various clinical disorders of humans as well as animals.
Clinical And Laboratory Studies On Equine Dermatophytosis
Benha Veterinary Medical Journal
In this study out of 200 Arabian horses periodically clinically examined in a station for Arabian horses, Cairo, Egypt, 55 animals (27.5%) had dermatological signs suggestive of dermatophytosis based on clinical examination. The majority of ringworm lesions were distributed over the head, neck, shoulder and limbs. Trichophyton mentagrophytes was the most commonly isolated dermatophyte species 19/39 (48.7 %), followed by T. verrucosum (12/39, 30.7%) and Microsporum canis (8/39, 20.9%). T. mentagrophytes and M. canis were isolated mostly from head and limbs. T. verrucosum was isolated from shoulder. The infection rate was higher in young horses less than one year old and increased during the winter season. Higher rate of infection was detected among female animals (81.4%) than males (60.7%). These findings have great veterinary and public health significance.
Seminars in Avian and Exotic Pet Medicine, 2000
Dermatophytes are fungi that can use keratin as a nutrient source. These organisms invade keratinized tissue (skin, hair, and nails) and cause dermatophytosis or ringworm. Colonization is usually restricted to the nonliving, cornified layer of the epidermis because of the inability of dermatophytes to penetrate viable tissue of an immunocompetent host. However, infection does elicit a host response ranging from mild to severe that is related to the species and strain of fungus. Zoophilic species are primarily parasitic on animals, and infections are often mild or symptomless in exotic pets. Infection with unusual or geophilic (soil-associated) dermatophytes causes severe inflammatory lesions. Ringworm has long been associated with rodents and rabbits: it is common in rabbits and guinea pigs; uncommon in chinchillas, mice, and rats; rare in golden and dwarf hamsters; and unreported in gerbils. Dermatophytosis is rare in ferrets and unreported in pet African pygmy hedgehogs. In naturally occurring infections, Trichophyton mentagrophytes is the fungal species most commonly isolated; Microsporum sp. are occasionally reported. Because dermatophytes cause a communicable disease, pets that are symptomless carriers represent a potential zoonotic source to their owners. Current methods of diagnosis and clinical management of dermatophytosis, including a survey of recent trends in therapy are presented.
Isolation and Molecular Detection of Dermatophytes from Horses in the Governorate of Wasit, Iraq
Annals of Tropical Medicine and Public Health, 2020
Dermatophytosis causes superficial fungal infection that poses public health problems to man and animals and economically significant causes of numerous dermatomes' that have been observed in equine species, therefore the aim of this study to investigate the distribution of dermatophytes species in horses and comparing the traditional methods with PCR techniques for detection of dermatophytes in the governorate of Wasit,Iraq. A total of 50 horses samples were collected from skin scrapings suggestive of dermatophytosis from diverse ages, sexes and areas, during the period of June to August 2018. The results showed that 32 (64%) out of 50 horses sampled were positive for dermatophytes by direct microscopic, while cultures only 26 (52%) out of 50 horses sampled were positive results for dermatophytes and results shown all culture positive samples were also positive in direct microscopic. In this study which exposed most frequent dermatophytes isolates were Trichophyton and Microsporum species in cultures and five species were recovered, T. equinium was the most common species 9 (34.6%) out of 26 positive culture, followed by T. mentagrophytes7(26.9%), M. equinium 5(19.3%), T. verrucosum 3(11.5%), M. gypseum 2(7.7%)and 6 isolated of non-dermatophytes were also detected. This study showed all culture positive samples were also positive in PCR technique. While six isolates were positive by direct microscopic show negative result by PCR technique. This indicates the infected of the skin with other types of non-dermatophytes. In conclusion that Trichophyton and Microsporum species were most frequently occurring dermatophytes in horses and PCR targeting the (Chs1) gene may be considered confirmed correct identification and screening test for detection of dermatophytes in horses.
Identification of dermatophytes isolated from ringworm lesions of camels
Suez Canal Veterinary Medical Journal. SCVMJ, 2013
Objective: phenotypic and genotypic identification of dermatophytes isolated from skin lesions of camels. In this study 70 specimens of hair and skin scrapings were taken from camels with skin lesions from El-Beheira Governorate (Junbway / Kom Hamada / El-Dalngat), El-Giza Governorate (Briqash) and El-Sharkia Governorate (Belbeis) for identification of common dermatophytes of camels using conventional techniques macromorphology , micromorphology and physiological test and compared with molecular method (polemerase chain reaction) using the short oligonucleotide (GACA)4 as a primer for identification of the tested dermatophyte isolates. (GACA) 4 based PCR identified the tested isolates from camel skin lesions to the species level in full agreement with the culture-based method.
Dermatophytosis in an adult cattle due to Trichophyton verrucosum
Dermatophytosis is the most commonly occurring highly infectious and contagious cutaneous mycosis of public health and economic significance. This paper describes the etiologic significance of Trichophton verrucosum in bovine dermatitis. Trichopyton verrucosum, a zoophilic dermatophyte, was identified in the cutaneous lesions of a crossbred cow by employing standard mycological techniques. The diagnosis was confirmed by demonstration of fungal agents in the skin lesions by direct microscopy in potassium hydroxide and ink solution, and also by isolation of Trichophyton verrucosum on Sabouraud dextrose agar supplemented with chloramphenicol, actidione and thiamine. The detailed microscopic morphology of the isolates of dermatophytic fungus in Narayan stain revealed septate hyphae, microcondia and macroconidia. The animal was treated with topical application of 2% solution of tincture iodine. Direct microscopy in KOH-INK solution is recommended for the rapid presumptive diagnosis of ringworm in rural areas, where laboratory facility of isolation of dermatophytes is not available. As animal dermatophytes are highly communicable to humans, care should be taken when dealing with diseased animals to avoid the infection. It is emphasized that Narayan stain should be widely used in microbiology and public health laboratories to study the morphology of dermatophytes and other fungi, which are implicated in the various clinical disorders of humans and animals.
Prevalence and distribution of dermatophytes among domestic horses in Kwara state, Nigeria
Balogun et al /Sokoto Journal of Veterinary Sciences (2017) 15(2): 1-6. Abstract The study investigated the prevalence and distribution of dermatophytes among domestic horses in Kwara state, Nigeria. A total of 91 samples were collected which comprised of skin scrapings and hair from both infected and asymptomatic animals. The highest dermatophyte isolation rate per total samples collected from each of the 7 different Local Government Area (LGAs) was 25% for Ilorin-East. Statistically significant differences (p < 0.05) were observed in the number of dermatophyte isolates obtained from the seven different LGAs. Dermatophytic lesions were observed on four anatomical sites of the body of horses that were sampled. These sites were the limbs, tail, head and abdominal region with dermatophyte isolation rate per total samples collected being 18.7%, 16%, 15% and 10%, respectively. Out of 85 male horses sampled, 12 were positive, and out of the six female horses sampled, two were positive. However, there was no statistically significant difference (p ˃ 0.05) between the total dermatophytes isolated from male (14.1%) or female (33.3%) horses from the seven LGAs in Kwara state. Dermatophytes isolated include Trichophyton tonsurans, Trichophyton verrucosum, Trichophyton soudanense, M. gypseum, Microsporum persicolor, Microsporum equinum and Microsporum fulvum with Trichophyton tonsurans and Trichophyton soudanense being anthropophilic.