The Capillary Pattern in Human Masseter Muscle During Ageing (original) (raw)
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The Effect of Aging on Skeletal Muscle Capillarization in a Murine Model
The Journals of Gerontology Series A: Biological Sciences and Medical Sciences, 1999
Capillarization 0/skeletal muscle has been reported to be both maintained and reduced with advancing age. This conftict may represent methodological differences between biopsy studies. We have examined capillarization throughout two muscles, soleus and extensor digitorum longus (EDL),/rom a well-established colony 0/aging mice, and related this to fiber number (CIF ratio) and type. Labeling 0/ muscle capillaries was performed with the biotinylated Griffonia (Bandeiraea) simplicifolia lectin (GSL I) using immunochemistry. The results showed a signijicant increase in the CIF ratio in the aged mice when compared with the younger (6-month mice soleus =1.296,95% CI 1.226-1.366 vs 28-month mice soleus =1.530,95% CI 1.488-1.572, P < .001; 6-month mice EDL =0.881,95% CI 0.751-1.011 vs 28-month mice EDL = 1.124,95% CI 1.028-1.220, p = .017). These differences could not be accountedfor by changes infiber type but may reflect loss 0/fibers. Alternatively, there may be increased angiogenic drive or a failure 0/downregulation 0/angioge1Jl!sis.
Characterization of the Capillary Network in Skeletal Muscles From 3D Data
Physiological Research, 2011
In this review we present immunohistochemical methods for visualization of capillaries and muscle fibres in thick muscle sections. Special attention is paid to the procedures that preserve good morphology. Applying confocal microscopy and virtual 3D stereological grids, or tracing of capillaries in virtual reality, length of capillaries within a muscle volume or length of capillaries adjacent to a muscle fibre per fibre length, fibre surface area or fibre volume can be evaluated by an unbiased approach. Moreover, 3D models of capillaries and muscle fibres can be produced. Comparison of the developed methods with counting capillary profiles from 2D sections is discussed and the reader is warned that counting capillary profiles from 2D sections can underestimate the capillary length by as much as 75 percent. Application of the described 3D methodology is illustrated by the anatomical remodelling of capillarity during acute denervation and early reinnervation in the rat soleus and exte...
Microvascular …, 1984
The stereomorphological arrangement of skeletal muscle capillaries depends on many factors. Genetic biochemical composition of muscle fibers, age, and training can interfere with the growth of the capillary network. This study is focused on the age effect of chronic reduction of blood flow, as in arterial stenosis, in skeletal muscle network of young rats when compared with adult rats. It has been observed that there is a statistically significant decrease of the length of short capillaries which cross the muscle fibers. In adult rats the opposite occurs.
Three-dimensional study of the capillary supply of skeletal muscle fibres using confocal microscopy
Journal of Muscle Research and Cell Motility, 2001
Three-dimensional (3D) study of capillary network of individual muscle fibres in rat extensor digitorum longus (EDL) and soleus (SOL) muscles is presented. Stereology and 3D reconstruction techniques were applied to stacks of serial optical sections recorded by a confocal microscope from thick muscle slices. The results suggest that SOL muscle fibres have a larger surface area and volume as well as a larger length of capillaries per fibre length than EDL. On the other hand, these two muscles have a similar ratio of capillary length to fibre surface area. The 3D approach to evaluation of muscle fibre capillarization brings many advantages over traditional measurements made on single muscle sections and could also be applied to the study of angiogenesis in other tissues.
A novel staining method for quantification and 3D visualisation of capillaries and muscle fibres
European Journal of Histochemistry, 2009
The aim of this study was to introduce a combined fluorescent staining that clearly demonstrates capillaries and distinguishes them from the basal lamina of muscle fibres in skeletal muscle tissue. The triple staining with CD31, Griffonia (Bandeira) simplicifolia lectin (GSL I) and laminin efficiently distinguishes vascular endothelium from the basal lamina of skeletal muscle fibres in physiological and pathological conditions. The presented triple staining method has several advantages, which facilitate quantitative analysis of the capillary network, and its relation to individual muscle fibres.
Coupling between skeletal muscle fiber size and capillarization is maintained during healthy aging
Journal of cachexia, sarcopenia and muscle, 2017
As muscle capillarization is related to the oxidative capacity of the muscle and the size of muscle fibres, capillary rarefaction may contribute to sarcopenia and functional impairment in older adults. Therefore, it is important to assess how ageing affects muscle capillarization and the interrelationship between fibre capillary supply with the oxidative capacity and size of the fibres. Muscle biopsies from healthy recreationally active young (22 years; 14 men and 5 women) and older (74 years; 22 men and 6 women) people were assessed for muscle capillarization and the distribution of capillaries with the method of capillary domains. Oxidative capacity of muscle fibres was assessed with quantitative histochemistry for succinate dehydrogenase (SDH) activity. There was no significant age-related reduction in muscle fibre oxidative capacity. Despite 18% type II fibre atrophy (P = 0.019) and 23% fewer capillaries per fibre (P < 0.002) in the old people, there was no significant differ...
2014
The aim of the study was to test the hypothesis that aging causes changes in fiber composition and vascular supply in the human masseter muscle that contribute to impaired jaw function in elderly. The myosin heavy chain (MyHC) composition and capillary supply of muscle fibers in functionally different parts of the masseter muscle of six elderly and six young subjects (mean age 74 and 22 years) were analyzed with immunohistochemical and morphological methods. The mean muscle fiber area in the old masseter was decreased by 27% compared to the young subjects (1100 vs. 1507 m, p=0.038). Smaller mean fiber area was observed for all fibers containing only slow MyHCI or fast MyHCII isoforms, but not for fibers coexpressing slow and fast MyHCs. There were no significant differences in the numbers of capillaries around fiber (CAF 1.85 vs. 1.92). When CAF was related to individual fiber area, capillaries around fiber area (CAFA), the capillary supply was significantly higher in elderly (CAFA...
Microvascular Research, 1990
It is generally assumed that when a muscle is shortened or extended the total length of capillaries does not change, implying that capillaries are nondistensible, longitudinally. On the basis of stereological estimates of capillary anisotropy versus sarcomere length, we propose that as long as capillaries are in a tortuous configuration muscle extension will merely decrease the tortuosity, leaving vessel length unaltered. Once capillaries have been pulled into a straight configuration, further extension of the muscle will cause the vessels to stretch. By means of intravital videomicroscopy we have demonstrated that stretching of individual capillaries does indeed occur over a sarcomere length range of 2.1 to 2.9 microns in rat extensor digitorum longus muscle. In vivo measurements of the lengths of six capillaries together with the sarcomere lengths of adjacent fibers were made in muscles positioned at various degrees of extension. Normalized data indicated that four capillaries stretched to the same degree as the muscle, one stretched more and another less. This may reflect differences in distensibility or tortuosity of capillaries in series with one another. The elastic stretching of capillaries during muscle activity may have important consequences in terms of shifts in permeability and increases in capillary surface area.