The genetic structure of red raspberry (Rubus idaeus L.) populations in Lithuania (original) (raw)
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Assessment of diversity of wild raspberries [Rubus idaeus L.] in Lithuania
2004
Twenty genotypes of wild red raspberry (Rubus idaeus L.) from the germplasm collection of the Botanical Garden of Vilnius University were analyzed for genetic diversity on the basis of random amplified polymorphic DNA markers. Initial screening with 44 oligonucleotide primers identified 36 primers corresponding to 285 RAPD loci. 80% of the loci detected were polymorphic. Each primer produced a mean of 7.9 bands. Indices of genetic distance between the genotypes ranged from 0.176 to 0.318. Eight of the primers produced ten or more informative PCR products. These primers promise to be useful in the further analysis of the R. idaeus germplasm collection. Data from RAPD fingerprints were subjected to factor analysis by the principal components method. The adaptive value of 230 polymorphic RAPD loci was assessed. Correlations between soil conditions and the results obtained by factor analysis were elaborated by calculating the Pearson correlation and its p-value. The only significant cor...
2018
The preliminary aim of the study before future breeding program was to reveal usability of ISSR markers to assess genetic diversity and relatedness within raspberry genotypes wildly grown in Northern Turkey. In this study, 19 wild raspberry genotypes collected from Black Sea region in Turkey were used. ISSR PCR analyses were carried on the DNA of genotypes isolated using mini CTAB extraction method. The levels of polymorphism between genotypes were determined using the UBC ISSR primers. A total of 111 bands were obtained from 15 UBC ISSR primers. Out of 111 bands, 101 bands were polymorphic. The number of bands obtained per primer ranged between 3 and 10, the average number of bands were determined as 7.4. The average number of polymorphic bands per primer was 6.73. Similarities and differences between genotypes have been studied at the molecular level. The data used for statistical analysis were obtained by the evaluation of ISSR bands. Similarity coefficient and UPGMA dendrogram w...
Assessment of Genetic Variability Among Raspberry Accessions Using Molecular Markers
Acta Scientiarum Polonorum Hortorum Cultus
In this study, random amplified polymorphic DNA (RAPD) and simple sequence repeat (SSR) loci were used to investigate the genetic relationships in a group of 22 raspberry accessions. Fifteen RAPD primers generated a total of 324 bands, among them 94.1% were polymorphic. From ten used SSR pairs of primers, nine generated only polymorphic bands and the average percentage of polymorphism was 97.8%. Genetic similarity indices calculated on the basis of RAPD and SSR data indicated a wide range of genetic variability of the analyzed raspberry collection. Cluster analysis by UPGMA (Unweighted Pair-Group Method with Arithmetic averaging) and PCA (Principal Component Analysis) clearly delineated the genetic relationships among all the accessions. The highest genetic similarity, determined on the basis of RAPD and SSR markers, was found between two Polish cultivars-'Polesie' and 'Polesie Żółte', whilst 'Jewel' from USA, belonging to Rubus occidentalis, was found to be the cultivar that varied most from all the accessions. The obtained results confirmed the usability of RAPD and SSR markers for discriminating among closely related raspberries and for determining the genetic variability among cultivars. It might be helpful for breeders to plan their breeding strategy.
Biotechnology & Biotechnological Equipment, 2005
The Bulgarian raspberry germplasm collection contains both Bulgarian and foreign varieties with valuable agronomic characters of commercial value. At present, mainly Bulgarian raspberry varieties are grown in Bulgaria with qualities famous in other European countries (Romania, Hungary, Poland, Russia etc.). The study of genetic diversity in the Bulgarian raspberry germplasm collection is mainly based on morphological descriptions. Morphological characteristics are important tools but they are not enough for evaluation of the genetic diversity due to the influence of environmental factors and low number of loci studied. The application of molecular markers should give an enable breeders to expand genetic diversity in breeding material and allow selection of new perspective forms in future breeding programmes. In this article we present our initial study on the assessment of genetic diversity among Bulgarian and foreign raspberry accessions using Simple Sequence Repeat (SSRs) markers. Sixteen raspberry cultivars (six Bulgarian, eight foreign and two wild accessions) were screened for their polymorphism at four loci using polymorphic microsatellite markers. The value determined for genetic variation demonstrated a high genetic diversity in the Bulgarian raspberry germplasm collection. Genetic diversity (GD) calculated from SSR data ranged from 0,740 to 0,887 with a mean
Assessment of Genetic Diversity among Selected Raspberry Cultivars
Growing raspberries in southern United States is a challenging task as they are high chill-loving plants. Cultivation of raspberry in Florida is significantly hampered due to prevailing hot and humid conditions. The characteristics of raspberry cultivars have been categorized based on morphological descriptions that are dependent on the environment. With this view our program is geared toward identifying low chill/high temperature raspberry genotypes. In this regard, genetic diversity among raspberry cultivars being evaluated in our breeding program for low-chill/high temperature tolerance was assessed employing molecular techniques. A high quality DNA was isolated from raspberry leaf tissue and subjected to molecular analysis. DNA typing was carried out using RAPD assay. Of the 100 primers screened, 10 primers yielded 142 usable DNA bands. Pairwise comparisons of unique and shared amplification products were used to construct a phylogenetic tree using Euclidean linkage distances that clearly separated low-and high-chill raspberry genotypes. These data showed the potentiality of RAPD markers for assessing genetic diversity among raspberry cultivars. Further studies coupled with field analysis would aid in identifying low-chill/high temperature fruit set raspberry genotypes useful for developing raspberry cultivars suitable for Florida.
AFLP-Based Genetic Relationships in Wild and Cultivated Red Raspberry Genotypes ( Rubus Idaeus L.)
Biotechnology & Biotechnological Equipment, 2008
The genus Rubus contains a large number of highly variably and heterogeneous species, which occur in all parts of the world except the desert regions. Turkey has notable wild grown Rubus populations and these fruits are a traditional part of Turkish diet. The leaves and fruits of wild Rubus forms are used for medicinal purposes in Turkey as well. In the present study we attempted to characterize 11 wild selections and 1 well known red raspberry cultivar, Heritage by using fluorescent dyed AFLP markers and capillary electrophoresis. Four primer combinations generated a total of 259 bands, 182 (69.0%) of which were polymorphic. The higher genetic similarity was found between AH2 and AH3 genotypes. UPGMA clustering of the accessions showed seven major groups. The cultivar Heritage independently formed its own group. AFLP profiles suggesting that the wild genotypes might be derived from seedlings rather than through clonally offshoot propagation.
Agronomy
Raspberry is a valuable berry crop containing a large amount of antioxidants that correlates with the color of the berries. We evaluated the genetic diversity of differently colored raspberry cultivars by the microsatellite markers developed using the flavonoid biosynthesis structural and regulatory genes. Among nine tested markers, seven were polymorphic. In total, 26 alleles were found at seven loci in 19 red (Rubus idaeus L.) and two black (R. occidentalis L.) raspberry cultivars. The most polymorphic marker was RiMY01 located in the MYB10 transcription factor intron region. Its polymorphic information content (PIC) equalled 0.82. The RiG001 marker that previously failed to amplify in blackberry also failed in black raspberry. The raspberry cultivar clustering in the UPGMA dendrogram was unrelated to geographical and genetic origin, but significantly correlated with the color of berries. The black raspberry cultivars had a higher homozygosity and clustered separately from other c...